Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1991 Oct;173(20):6626–6631. doi: 10.1128/jb.173.20.6626-6631.1991

Klebsiella aerogenes catabolite gene activator protein and the gene encoding it (crp).

R Osuna 1, R A Bender 1
PMCID: PMC209001  PMID: 1655718

Abstract

The catabolite gene activator protein from Klebsiella aerogenes (CAPK) and the corresponding protein from Escherichia coli (CAPE) were shown to be nearly identical. Both CAPK and CAPE activated transcription from the CAP-dependent promoters derived from E. coli and K. aerogenes. The crp gene from K. aerogenes (encoding CAP) is tightly linked to rpsL. The nucleotide sequence of crp predicts an amino acid sequence for CAPK that differs in only one position from that of CAPE.

Full text

PDF
6626

Images in this article

6628FIG. 2
on p.6628
6629FIG. 3
on p.6629
6629FIG. 4
on p.6629
6630FIG. 5
on p.6630

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aiba H., Fujimoto S., Ozaki N. Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein. Nucleic Acids Res. 1982 Feb 25;10(4):1345–1361. doi: 10.1093/nar/10.4.1345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alper M. D., Ames B. N. Transport of antibiotics and metabolite analogs by systems under cyclic AMP control: positive selection of Salmonella typhimurium cya and crp mutants. J Bacteriol. 1978 Jan;133(1):149–157. doi: 10.1128/jb.133.1.149-157.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baldauf S. L., Cardani M. A., Bender R. A. Regulation of the galactose-inducible lac operon and the histidine utilization operons in pts mutants of Klebsiella aerogenes. J Bacteriol. 1988 Dec;170(12):5588–5593. doi: 10.1128/jb.170.12.5588-5593.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bender R. A., Janssen K. A., Resnick A. D., Blumenberg M., Foor F., Magasanik B. Biochemical parameters of glutamine synthetase from Klebsiella aerogenes. J Bacteriol. 1977 Feb;129(2):1001–1009. doi: 10.1128/jb.129.2.1001-1009.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bender R. A., Streicher S. L. Glutamine synthetase regulation, adenylylation state, and strain specificity analyzed by polyacrylamide gel electrophoresis. J Bacteriol. 1979 Feb;137(2):1000–1007. doi: 10.1128/jb.137.2.1000-1007.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Busby S., Aiba H., de Crombrugghe B. Mutations in the Escherichia coli operon that define two promoters and the binding site of the cyclic AMP receptor protein. J Mol Biol. 1982 Jan 15;154(2):211–227. doi: 10.1016/0022-2836(82)90061-4. [DOI] [PubMed] [Google Scholar]
  8. Buvinger W. E., Riley M. Nucleotide sequence of Klebsiella pneumoniae lac genes. J Bacteriol. 1985 Sep;163(3):850–857. doi: 10.1128/jb.163.3.850-857.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cossart P., Gicquel-Sanzey B. Cloning and sequence of the crp gene of Escherichia coli K 12. Nucleic Acids Res. 1982 Feb 25;10(4):1363–1378. doi: 10.1093/nar/10.4.1363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cossart P., Groisman E. A., Serre M. C., Casadaban M. J., Gicquel-Sanzey B. crp genes of Shigella flexneri, Salmonella typhimurium, and Escherichia coli. J Bacteriol. 1986 Aug;167(2):639–646. doi: 10.1128/jb.167.2.639-646.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fried M. G., Crothers D. M. CAP and RNA polymerase interactions with the lac promoter: binding stoichiometry and long range effects. Nucleic Acids Res. 1983 Jan 11;11(1):141–158. doi: 10.1093/nar/11.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fried M., Crothers D. M. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res. 1981 Dec 11;9(23):6505–6525. doi: 10.1093/nar/9.23.6505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Garner M. M., Revzin A. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system. Nucleic Acids Res. 1981 Jul 10;9(13):3047–3060. doi: 10.1093/nar/9.13.3047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Garner M. M., Revzin A. Stoichiometry of catabolite activator protein/adenosine cyclic 3',5'-monophosphate interactions at the lac promoter of Escherichia coli. Biochemistry. 1982 Nov 23;21(24):6032–6036. doi: 10.1021/bi00267a001. [DOI] [PubMed] [Google Scholar]
  15. Goldberg R. B., Bloom F. R., Magasanik B. Regulation of histidase synthesis in intergeneric hybrids of enteric bacteria. J Bacteriol. 1976 Jul;127(1):114–119. doi: 10.1128/jb.127.1.114-119.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Groisman E. A., Casadaban M. J. Mini-mu bacteriophage with plasmid replicons for in vivo cloning and lac gene fusing. J Bacteriol. 1986 Oct;168(1):357–364. doi: 10.1128/jb.168.1.357-364.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Harris J. D., Martinez I. I., Calendar R. A gene from Escherichia coli affecting the sigma subunit of RNA polymerase. Proc Natl Acad Sci U S A. 1977 May;74(5):1836–1840. doi: 10.1073/pnas.74.5.1836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nakamura Y., Osawa T., Yura T. Chromosomal location of a structural gene for the RNA polymerase sigma factor in Escherichia coli. Proc Natl Acad Sci U S A. 1977 May;74(5):1831–1835. doi: 10.1073/pnas.74.5.1831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nieuwkoop A. J., Baldauf S. A., Hudspeth M. E., Bender R. A. Bidirectional promoter in the hut(P) region of the histidine utilization (hut) operons from Klebsiella aerogenes. J Bacteriol. 1988 May;170(5):2240–2246. doi: 10.1128/jb.170.5.2240-2246.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nieuwkoop A. J., Bender R. A. RNA polymerase as a repressor of transcription in the hut(P) region of mutant Klebsiella aerogenes histidine utilization operons. J Bacteriol. 1988 Oct;170(10):4986–4990. doi: 10.1128/jb.170.10.4986-4990.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nieuwkoop A. J., Boylan S. A., Bender R. A. Regulation of hutUH operon expression by the catabolite gene activator protein-cyclic AMP complex in Klebsiella aerogenes. J Bacteriol. 1984 Sep;159(3):934–939. doi: 10.1128/jb.159.3.934-939.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Osuna R., Boylan S. A., Bender R. A. In vitro transcription of the histidine utilization (hutUH) operon from Klebsiella aerogenes. J Bacteriol. 1991 Jan;173(1):116–123. doi: 10.1128/jb.173.1.116-123.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Prival M. J., Magasanik B. Resistance to catabolite repression of histidase and proline oxidase during nitrogen-limited growth of Klebsiella aerogenes. J Biol Chem. 1971 Oct 25;246(20):6288–6296. [PubMed] [Google Scholar]
  24. Sabourin D., Beckwith J. Deletion of the Escherichia coli crp gene. J Bacteriol. 1975 Apr;122(1):338–340. doi: 10.1128/jb.122.1.338-340.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shanblatt S. H., Revzin A. Two catabolite activator protein molecules bind to the galactose promoter region of Escherichia coli in the presence of RNA polymerase. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1594–1598. doi: 10.1073/pnas.80.6.1594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Streicher S. L., Bender R. A., Magasanik B. Genetic control of glutamine synthetase in Klebiella aerogenes. J Bacteriol. 1975 Jan;121(1):320–331. doi: 10.1128/jb.121.1.320-331.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Taniguchi T., O'Neill M., de Crombrugghe B. Interaction site of Escherichia coli cyclic AMP receptor protein on DNA of galactose operon promoters. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5090–5094. doi: 10.1073/pnas.76.10.5090. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES