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. 1990 Jun;172(6):3264–3268. doi: 10.1128/jb.172.6.3264-3268.1990

Isolation and characterization of the 5S rRNA gene of Leptospira interrogans.

M Fukunaga 1, I Horie 1, I Mifuchi 1
PMCID: PMC209134  PMID: 2345145

Abstract

The gene encoding the 5S rRNA for Leptospira interrogans serovar canicola strain Moulton was isolated and sequenced. The 5S rRNA gene occurs as a single copy within the genome and encodes a 117-nucleotide-long RNA molecule. The 5S rRNA gene is flanked at both the 5' and 3' ends by regions of A + T-rich sequences, and the 5'-flanking region contains a promoter sequence. L. interrogans has a unique and remarkable organization of the 5S rRNA gene. The 5S rRNA molecule exhibits a strong similarity to typical eubacterial 5S rRNA in terms of overall secondary structure, while the primary sequence is conserved to a lesser degree. Restriction analysis of the 5S rRNA gene indicated that the DNA sequence including the 5S rRNA gene is highly conserved in the genomes of parasitic leptospires.

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Selected References

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