Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1989 Jan;171(1):342–348. doi: 10.1128/jb.171.1.342-348.1989

Mutants of Escherichia coli deficient in the fermentative lactate dehydrogenase.

F Mat-Jan 1, K Y Alam 1, D P Clark 1
PMCID: PMC209593  PMID: 2644194

Abstract

Mutants of Escherichia coli deficient in the fermentative NAD-linked lactate dehydrogenase (ldh) have been isolated. These mutants showed no growth defects under anaerobic conditions unless present together with a defect in pyruvate formate lyase (pfl). Double mutants (pfl ldh) were unable to grow anaerobically on glucose or other sugars even when supplemented with acetate, whereas pfl mutants can do so. The ldh mutation was found to map at 30.5 min on the E. coli chromosome. The ldh mutant FMJ39 showed no detectable lactate dehydrogenase activity and produced no lactic acid from glucose under anaerobic conditions as estimated by in vivo nuclear magnetic resonance measurements. We also found that in wild-type strains the fermentative lactate dehydrogenase was conjointly induced by anaerobic conditions and an acidic pH. Despite previous findings that phosphate concentrations affect the proportion of lactic acid produced during fermentation, we were unable to find any intrinsic effect of phosphate on lactate dehydrogenase activity, apart from the buffering effect of this ion.

Full text

PDF
342

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdulrashid N., Clark D. P. Isolation and genetic analysis of mutations allowing the degradation of furans and thiophenes by Escherichia coli. J Bacteriol. 1987 Mar;169(3):1267–1271. doi: 10.1128/jb.169.3.1267-1271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anilionis A., Ostapchuk P., Riley M. Identification of a second cryptic lambdoid prophage locus in the E. coli K12 chromosome. Mol Gen Genet. 1980;180(2):479–481. doi: 10.1007/BF00425865. [DOI] [PubMed] [Google Scholar]
  3. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Caddick M. X., Brownlee A. G., Arst H. N., Jr Regulation of gene expression by pH of the growth medium in Aspergillus nidulans. Mol Gen Genet. 1986 May;203(2):346–353. doi: 10.1007/BF00333978. [DOI] [PubMed] [Google Scholar]
  5. Clark D. P., Cronan J. E., Jr Acetaldehyde coenzyme A dehydrogenase of Escherichia coli. J Bacteriol. 1980 Oct;144(1):179–184. doi: 10.1128/jb.144.1.179-184.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clark D. P., Cunningham P. R., Reams S. G., Mat-Jan F., Mohammedkhani R., Williams C. R. Mutants of Escherichia coli defective in acid fermentation. Appl Biochem Biotechnol. 1988 Apr;17:163–173. doi: 10.1007/BF02779155. [DOI] [PubMed] [Google Scholar]
  7. Clark D., Cronan J. E., Jr Escherichia coli mutants with altered control of alcohol dehydrogenase and nitrate reductase. J Bacteriol. 1980 Jan;141(1):177–183. doi: 10.1128/jb.141.1.177-183.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cunningham P. R., Clark D. P. The use of suicide substrates to select mutants of Escherichia coli lacking enzymes of alcohol fermentation. Mol Gen Genet. 1986 Dec;205(3):487–493. doi: 10.1007/BF00338087. [DOI] [PubMed] [Google Scholar]
  9. Fouts K. E., Barbour S. D. Insertion of transposons through the major cotransduction gap of Escherichia coli K-12. J Bacteriol. 1982 Jan;149(1):106–113. doi: 10.1128/jb.149.1.106-113.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fouts K. E., Barbour S. D. Transductional mapping of ksgB and a new Tn5-induced kasugamycin resistance gene, ksgD, in Escherichia coli K-12. J Bacteriol. 1981 Feb;145(2):914–919. doi: 10.1128/jb.145.2.914-919.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HAUGAARD N. D- and L-lactic acid oxidases of Escherichia coli. Biochim Biophys Acta. 1959 Jan;31(1):66–72. doi: 10.1016/0006-3002(59)90439-1. [DOI] [PubMed] [Google Scholar]
  12. Ingledew W. J., Poole R. K. The respiratory chains of Escherichia coli. Microbiol Rev. 1984 Sep;48(3):222–271. doi: 10.1128/mr.48.3.222-271.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kline E. S., Mahler H. R. The lactic dehydrogenases of E. coli. Ann N Y Acad Sci. 1965 Jul 31;119(3):905–919. doi: 10.1111/j.1749-6632.1965.tb47451.x. [DOI] [PubMed] [Google Scholar]
  14. Ogino T., Arata Y., Fujiwara S. Proton correlation nuclear magnetic resonance study of metabolic regulations and pyruvate transport in anaerobic Escherichia coli cells. Biochemistry. 1980 Aug 5;19(16):3684–3691. doi: 10.1021/bi00557a008. [DOI] [PubMed] [Google Scholar]
  15. Ogino T., Arata Y., Fujiwara S., Shoun H., Beppu T. Proton correlation nuclear magnetic resonance study of anaerobic metabolism of Escherichia coli. Biochemistry. 1978 Oct 31;17(22):4742–4745. doi: 10.1021/bi00615a022. [DOI] [PubMed] [Google Scholar]
  16. Pecher A., Zinoni F., Böck A. The seleno-polypeptide of formic dehydrogenase (formate hydrogen-lyase linked) from Escherichia coli: genetic analysis. Arch Microbiol. 1985 May;141(4):359–363. doi: 10.1007/BF00428850. [DOI] [PubMed] [Google Scholar]
  17. Pecher A., Zinoni F., Jatisatienr C., Wirth R., Hennecke H., Böck A. On the redox control of synthesis of anaerobically induced enzymes in enterobacteriaceae. Arch Microbiol. 1983 Nov;136(2):131–136. doi: 10.1007/BF00404787. [DOI] [PubMed] [Google Scholar]
  18. Shaw D. J., Rice D. W., Guest J. R. Homology between CAP and Fnr, a regulator of anaerobic respiration in Escherichia coli. J Mol Biol. 1983 May 15;166(2):241–247. doi: 10.1016/s0022-2836(83)80011-4. [DOI] [PubMed] [Google Scholar]
  19. Shaw L., Grau F., Kaback H. R., Hong J. S., Walsh C. Vinylglycolate resistance in Escherichia coli. J Bacteriol. 1975 Mar;121(3):1047–1055. doi: 10.1128/jb.121.3.1047-1055.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Slonczewski J. L., Gonzalez T. N., Bartholomew F. M., Holt N. J. Mu d-directed lacZ fusions regulated by low pH in Escherichia coli. J Bacteriol. 1987 Jul;169(7):3001–3006. doi: 10.1128/jb.169.7.3001-3006.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stokes J. L. FERMENTATION OF GLUCOSE BY SUSPENSIONS OF ESCHERICHIA COLI. J Bacteriol. 1949 Feb;57(2):147–158. doi: 10.1128/jb.57.2.147-158.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Strathern A., Herskowitz I. Defective prophage in Escherichia coli K12 strains. Virology. 1975 Sep;67(1):136–143. doi: 10.1016/0042-6822(75)90411-0. [DOI] [PubMed] [Google Scholar]
  23. Tarmy E. M., Kaplan N. O. Chemical characterization of D-lactate dehydrogenase from Escherichia coli B. J Biol Chem. 1968 May 25;243(10):2579–2586. [PubMed] [Google Scholar]
  24. Tarmy E. M., Kaplan N. O. Interacting binding sites of L-specific lactic dehydrogenase of Escherichia coli. Biochem Biophys Res Commun. 1965 Nov 22;21(4):379–383. doi: 10.1016/0006-291x(65)90205-6. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES