Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1989 Feb;171(2):636–642. doi: 10.1128/jb.171.2.636-642.1989

rhs gene family of Escherichia coli K-12.

A B Sadosky 1, A Davidson 1, R J Lin 1, C W Hill 1
PMCID: PMC209644  PMID: 2644231

Abstract

Two additional members of a novel Escherichia coli gene family, the rhs genes, have been cloned and characterized. The structures of these loci, rhsC and rhsD, have been compared with those of rhsA and rhsB. All four loci contain a homologous 3.7-kilobase-pair core. Sequence comparison of the first 300 nucleotides of the cores showed that rhsA, rhsB, and rhsC are closely related, with only 1 to 2% sequence divergence, whereas rhsD is 18% divergent from the others. The beginning of the core coincides with the initiation of an open reading frame that extends beyond the 300 nucleotides compared. Whether a protein product is produced from this open reading frame has not been established. However, nucleotide substitutions which differentiate the cores have highly conservative effects on the predicted protein products; this suggests that products are made from the open reading frame and are under severe selection. The four rhs loci have been placed on both the genetic and restriction maps of E. coli K-12. A fifth rhs locus remains to be characterized. In terms of size, number, and sequence conservation, the rhs genes make up one of the most significant repetitions in E. coli, comparable to the rRNA operons.

Full text

PDF
636

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bolivar F. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. Gene. 1978 Oct;4(2):121–136. doi: 10.1016/0378-1119(78)90025-2. [DOI] [PubMed] [Google Scholar]
  3. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  4. Brody H., Greener A., Hill C. W. Excision and reintegration of the Escherichia coli K-12 chromosomal element e14. J Bacteriol. 1985 Mar;161(3):1112–1117. doi: 10.1128/jb.161.3.1112-1117.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brody H., Hill C. W. Attachment site of the genetic element e14. J Bacteriol. 1988 May;170(5):2040–2044. doi: 10.1128/jb.170.5.2040-2044.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Capage M. A., Scott J. R. SOS induction by P1 Km miniplasmids. J Bacteriol. 1983 Aug;155(2):473–480. doi: 10.1128/jb.155.2.473-480.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cedar H., Schwartz J. H. The asparagine synthetase of Escherhic coli. I. Biosynthetic role of the enzyme, purification, and characterization of the reaction products. J Biol Chem. 1969 Aug 10;244(15):4112–4121. [PubMed] [Google Scholar]
  8. Elvin C. M., Dixon N. E., Rosenberg H. Molecular cloning of the phosphate (inorganic) transport (pit) gene of Escherichia coli K12. Identification of the pit+ gene product and physical mapping of the pit-gor region of the chromosome. Mol Gen Genet. 1986 Sep;204(3):477–484. doi: 10.1007/BF00331028. [DOI] [PubMed] [Google Scholar]
  9. Filip C. C., Allen J. S., Gustafson R. A., Allen R. G., Walker J. R. Bacterial cell division regulation: characterization of the dnaH locus of Escherichia coli. J Bacteriol. 1974 Aug;119(2):443–449. doi: 10.1128/jb.119.2.443-449.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Greener A., Hill C. W. Identification of a novel genetic element in Escherichia coli K-12. J Bacteriol. 1980 Oct;144(1):312–321. doi: 10.1128/jb.144.1.312-321.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Guest J. R. Hybrid plasmids containing the citrate synthase gene (gltA) of Escherichia coli K12. J Gen Microbiol. 1981 May;124(1):17–23. doi: 10.1099/00221287-124-1-17. [DOI] [PubMed] [Google Scholar]
  12. Hadley R. G., Hu M., Timmons M., Yun K., Deonier R. C. A partial restriction map of the proA-purE region of the Escherichia coli K12 chromosome. Gene. 1983 May-Jun;22(2-3):281–287. doi: 10.1016/0378-1119(83)90113-0. [DOI] [PubMed] [Google Scholar]
  13. Herbert A. A., Guest J. R. Biochemical and genetic studies with lysine+methionine mutants of Escherichia coli: lipoic acid and alpha-ketoglutarate dehydrogenase-less mutants. J Gen Microbiol. 1968 Oct;53(3):363–381. doi: 10.1099/00221287-53-3-363. [DOI] [PubMed] [Google Scholar]
  14. Hill C. W., Foulds J., Soll L., Berg P. Instability of a missense suppressor resulting from a duplication of genetic material. J Mol Biol. 1969 Feb 14;39(3):563–581. doi: 10.1016/0022-2836(69)90146-6. [DOI] [PubMed] [Google Scholar]
  15. Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. doi: 10.1016/0092-8674(87)90503-4. [DOI] [PubMed] [Google Scholar]
  16. Lee C. A., Saier M. H., Jr Use of cloned mtl genes of Escherichia coli to introduce mtl deletion mutations into the chromosome. J Bacteriol. 1983 Feb;153(2):685–692. doi: 10.1128/jb.153.2.685-692.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lin R. J., Capage M., Hill C. W. A repetitive DNA sequence, rhs, responsible for duplications within the Escherichia coli K-12 chromosome. J Mol Biol. 1984 Jul 25;177(1):1–18. doi: 10.1016/0022-2836(84)90054-8. [DOI] [PubMed] [Google Scholar]
  18. Norrander J., Kempe T., Messing J. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983 Dec;26(1):101–106. doi: 10.1016/0378-1119(83)90040-9. [DOI] [PubMed] [Google Scholar]
  19. Yamamoto K., Satake M., Shinagawa H., Fujiwara Y. Amelioration of the ultraviolet sensitivity of an Escherichia coli recA mutant in the dark by photoreactivating enzyme. Mol Gen Genet. 1983;190(3):511–515. doi: 10.1007/BF00331084. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES