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. 1989 Feb;171(2):1214–1218. doi: 10.1128/jb.171.2.1214-1218.1989

Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome.

S Tabata 1, A Higashitani 1, M Takanami 1, K Akiyama 1, Y Kohara 1, Y Nishimura 1, A Nishimura 1, S Yasuda 1, Y Hirota 1
PMCID: PMC209726  PMID: 2644229

Abstract

A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.

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Selected References

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