Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1989 Apr;171(4):1835–1840. doi: 10.1128/jb.171.4.1835-1840.1989

Novel change in the carbohydrate portion of Myxococcus xanthus lipopolysaccharide during development.

S M Panasenko 1, B Jann 1, K Jann 1
PMCID: PMC209829  PMID: 2495265

Abstract

We have examined the alterations in lipopolysaccharide during aggregation and early development in Myxococcus xanthus. The lipopolysaccharide was isolated and characterized from cells developing on agar during glycerol induction and vegetative growth. A methylated amino sugar was identified as 6-O-methylgalactosamine by gas-liquid chromatography-mass spectrometry. This novel sugar was enriched in cells developing on agar.

Full text

PDF
1838

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Campos J. M., Geisselsoder J., Zusman D. R. Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus. J Mol Biol. 1978 Feb 25;119(2):167–178. doi: 10.1016/0022-2836(78)90431-x. [DOI] [PubMed] [Google Scholar]
  2. Cantoni G. L. Biological methylation: selected aspects. Annu Rev Biochem. 1975;44:435–451. doi: 10.1146/annurev.bi.44.070175.002251. [DOI] [PubMed] [Google Scholar]
  3. Carlson R. W., Kalembasa S., Turowski D., Pachori P., Noel K. D. Characterization of the lipopolysaccharide from a Rhizobium phaseoli mutant that is defective in infection thread development. J Bacteriol. 1987 Nov;169(11):4923–4928. doi: 10.1128/jb.169.11.4923-4928.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carlson R. W., Shatters R., Duh J. L., Turnbull E., Hanley B., Rolfe B. G., Djordjevic M. A. The Isolation and Partial Characterization of the Lipopolysaccharides from Several Rhizobium trifolii Mutants Affected in Root Hair Infection. Plant Physiol. 1987 Jun;84(2):421–427. doi: 10.1104/pp.84.2.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edelman G. M. Cell adhesion molecules. Science. 1983 Feb 4;219(4584):450–457. doi: 10.1126/science.6823544. [DOI] [PubMed] [Google Scholar]
  6. Fink J. M., Zissler J. F. Defects in motility and development of Myxococcus xanthus lipopolysaccharide mutants. J Bacteriol. 1989 Apr;171(4):2042–2048. doi: 10.1128/jb.171.4.2042-2048.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goldman R. C., Leive L. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2. Eur J Biochem. 1980;107(1):145–153. doi: 10.1111/j.1432-1033.1980.tb04635.x. [DOI] [PubMed] [Google Scholar]
  8. Hagen D. C., Bretscher A. P., Kaiser D. Synergism between morphogenetic mutants of Myxococcus xanthus. Dev Biol. 1978 Jun;64(2):284–296. doi: 10.1016/0012-1606(78)90079-9. [DOI] [PubMed] [Google Scholar]
  9. Hazelbauer G. L. Role of the receptor for bacteriophage lambda in the functioning of the maltose chemoreceptor of Escherichia coli. J Bacteriol. 1975 Oct;124(1):119–126. doi: 10.1128/jb.124.1.119-126.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaiser D. Social gliding is correlated with the presence of pili in Myxococcus xanthus. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5952–5956. doi: 10.1073/pnas.76.11.5952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Maloney D. H., Ballou C. E. Polymethylpolysaccharide synthesis in an ethionine-resistant mutant of Mycobacterium smegmatis. J Bacteriol. 1980 Mar;141(3):1217–1221. doi: 10.1128/jb.141.3.1217-1221.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mayer H., Framberg K., Weckesser J. 6-O-methyl-D-glucosamine in lipopolysaccharides of Rhodopseudomonas palustris strains. Eur J Biochem. 1974 May 2;44(1):181–187. doi: 10.1111/j.1432-1033.1974.tb03471.x. [DOI] [PubMed] [Google Scholar]
  14. Niedermeier W. Gas chromatography of neutral and amino sugars in glycoproteins. Anal Biochem. 1971 Apr;40(2):465–475. doi: 10.1016/0003-2697(71)90407-6. [DOI] [PubMed] [Google Scholar]
  15. Palva E. T., Mäkelä P. H. Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Eur J Biochem. 1980;107(1):137–143. doi: 10.1111/j.1432-1033.1980.tb04634.x. [DOI] [PubMed] [Google Scholar]
  16. Panasenko S. M. Methylation of macromolecules during development in Myxococcus xanthus. J Bacteriol. 1985 Nov;164(2):495–500. doi: 10.1128/jb.164.2.495-500.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Peterson A. A., McGroarty E. J. High-molecular-weight components in lipopolysaccharides of Salmonella typhimurium, Salmonella minnesota, and Escherichia coli. J Bacteriol. 1985 May;162(2):738–745. doi: 10.1128/jb.162.2.738-745.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. STROMINGER J. L., PARK J. T., THOMPSON R. E. Composition of the cell wall of Staphylococcus aureus: its relation to the mechanism of action of penicillin. J Biol Chem. 1959 Dec;234:3263–3268. [PubMed] [Google Scholar]
  19. Shimkets L. J., Kaiser D. Induction of coordinated movement of Myxococcus xanthus cells. J Bacteriol. 1982 Oct;152(1):451–461. doi: 10.1128/jb.152.1.451-461.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Smith W. L., Ballou C. E. The 6-O-methylglucose-containing lipopolysaccharides of Mycobacterium phlei. Locations of the neutral and acidic acyl groups. J Biol Chem. 1973 Oct 25;248(20):7118–7125. [PubMed] [Google Scholar]
  21. Sutherland I. W., Thomson S. Comparison of polysaccharides produced by Myxococcus strains. J Gen Microbiol. 1975 Jul;89(1):124–132. doi: 10.1099/00221287-89-1-124. [DOI] [PubMed] [Google Scholar]
  22. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  23. WARAVDEKAR V. S., SASLAW L. D. A sensitive colorimetric method for the estimation of 2-deoxy sugars with the use of the malonaldehyde-thiobarbituric acid reaction. J Biol Chem. 1959 Aug;234(8):1945–1950. [PubMed] [Google Scholar]
  24. Weckesser J., Rosenfelder G., Mayer H., Lüderitz O. The identification of 3-O-methyl-D-xylose and 3-O-methyl-L-xylose as constituents of the lipopolysaccharides of Myxococcus fulvus and Rhodopseudomonas viridis, respectively. Eur J Biochem. 1971 Dec 22;24(1):112–115. doi: 10.1111/j.1432-1033.1971.tb19660.x. [DOI] [PubMed] [Google Scholar]
  25. Whiton R. S., Lau P., Morgan S. L., Gilbart J., Fox A. Modifications in the alditol acetate method for analysis of muramic acid and other neutral and amino sugars by capillary gas chromatography-mass spectrometry with selected ion monitoring. J Chromatogr. 1985 Oct 25;347(1):109–120. doi: 10.1016/s0021-9673(01)95474-3. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES