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. 1989 Apr;171(4):1846–1853. doi: 10.1128/jb.171.4.1846-1853.1989

Deletion hot spots in chimeric Escherichia coli plasmids.

B Michel 1, E D'Alençon 1, S D Ehrlich 1
PMCID: PMC209831  PMID: 2649476

Abstract

Deletions form frequently in chimeric plasmids composed of M13mp2, pBR322, and pC194 (B. Michel and S. D. Ehrlich, Proc. Natl. Acad. Sci. USA 83:3386-3390, 1986). They are generated by joining of the nucleotide neighboring the nick site in the M13 replication origin to a nonadjacent nucleotide. This nucleotide is most often located within particular short plasmid regions, named deletion hot spots. Three natural hot spots were present in the chimeric plasmids. Two were active only when the DNA replication initiated at the M13 origin was allowed to progress; the third was active only in the presence of wild-type amounts of DNA ligase. Three artificial hot spots were generated by creating palindromic sequences in the plasmids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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