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. 1989 Jul;171(7):4063–4066. doi: 10.1128/jb.171.7.4063-4066.1989

Flagellation of Pseudomonas putida and analysis of its motile behavior.

C S Harwood 1, K Fosnaugh 1, M Dispensa 1
PMCID: PMC210162  PMID: 2738028

Abstract

Pseudomonas putida flagella were examined. Also, changes in motile behavior in response to chemoattractants were analyzed quantitatively by computer. Reversals in the rotation direction of bundles of polar flagella resulted in changes in swimming direction. Cells swimming in buffer changed direction once every 2 s on average, whereas cells exposed to the attractant benzoate changed direction an average of once every 10 s. The findings show that P. putida responds to temporal gradients of chemoattractant by suppressing changes in the direction of rotation of flagella.

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Selected References

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  1. Ashby A. M., Watson M. D., Loake G. J., Shaw C. H. Ti plasmid-specified chemotaxis of Agrobacterium tumefaciens C58C1 toward vir-inducing phenolic compounds and soluble factors from monocotyledonous and dicotyledonous plants. J Bacteriol. 1988 Sep;170(9):4181–4187. doi: 10.1128/jb.170.9.4181-4187.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berg H. C., Brown D. A. Chemotaxis in Escherichia coli analysed by three-dimensional tracking. Nature. 1972 Oct 27;239(5374):500–504. doi: 10.1038/239500a0. [DOI] [PubMed] [Google Scholar]
  3. Fosnaugh K., Greenberg E. P. Motility and chemotaxis of Spirochaeta aurantia: computer-assisted motion analysis. J Bacteriol. 1988 Apr;170(4):1768–1774. doi: 10.1128/jb.170.4.1768-1774.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goldman D. J., Ordal G. W. Sensory adaptation and deadaptation by Bacillus subtilis. J Bacteriol. 1981 Jul;147(1):267–270. doi: 10.1128/jb.147.1.267-270.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Götz R., Schmitt R. Rhizobium meliloti swims by unidirectional, intermittent rotation of right-handed flagellar helices. J Bacteriol. 1987 Jul;169(7):3146–3150. doi: 10.1128/jb.169.7.3146-3150.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harwood C. S., Rivelli M., Ornston L. N. Aromatic acids are chemoattractants for Pseudomonas putida. J Bacteriol. 1984 Nov;160(2):622–628. doi: 10.1128/jb.160.2.622-628.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Khan S., Macnab R. M. The steady-state counterclockwise/clockwise ratio of bacterial flagellar motors is regulated by protonmotive force. J Mol Biol. 1980 Apr 15;138(3):563–597. doi: 10.1016/s0022-2836(80)80018-0. [DOI] [PubMed] [Google Scholar]
  8. Kihara M., Macnab R. M. Cytoplasmic pH mediates pH taxis and weak-acid repellent taxis of bacteria. J Bacteriol. 1981 Mar;145(3):1209–1221. doi: 10.1128/jb.145.3.1209-1221.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LAUTROP H., JESSEN O. ON THE DISTINCTION BETWEEN POLAR MONOTRICHOUS AND LOPHOTRICHOUS FLAGELLATION IN GREEN FLUORESCENT PSEUDOMONADS. Acta Pathol Microbiol Scand. 1964;60:588–598. doi: 10.1111/apm.1964.60.4.588. [DOI] [PubMed] [Google Scholar]
  10. Larsen S. H., Reader R. W., Kort E. N., Tso W. W., Adler J. Change in direction of flagellar rotation is the basis of the chemotactic response in Escherichia coli. Nature. 1974 May 3;249(452):74–77. doi: 10.1038/249074a0. [DOI] [PubMed] [Google Scholar]
  11. Macnab R. M. Bacterial flagella rotating in bundles: a study in helical geometry. Proc Natl Acad Sci U S A. 1977 Jan;74(1):221–225. doi: 10.1073/pnas.74.1.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Macnab R. M. Examination of bacterial flagellation by dark-field microscopy. J Clin Microbiol. 1976 Sep;4(3):258–265. doi: 10.1128/jcm.4.3.258-265.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Macnab R. M., Koshland D. E., Jr The gradient-sensing mechanism in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2509–2512. doi: 10.1073/pnas.69.9.2509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ornston L. N., Parke D. Properties of an inducible uptake system for beta-ketoadipate in Pseudomonas putida. J Bacteriol. 1976 Feb;125(2):475–488. doi: 10.1128/jb.125.2.475-488.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Parke D., Ornston L. N., Nester E. W. Chemotaxis to plant phenolic inducers of virulence genes is constitutively expressed in the absence of the Ti plasmid in Agrobacterium tumefaciens. J Bacteriol. 1987 Nov;169(11):5336–5338. doi: 10.1128/jb.169.11.5336-5338.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Parke D., Rivelli M., Ornston L. N. Chemotaxis to aromatic and hydroaromatic acids: comparison of Bradyrhizobium japonicum and Rhizobium trifolii. J Bacteriol. 1985 Aug;163(2):417–422. doi: 10.1128/jb.163.2.417-422.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sager B. M., Sekelsky J. J., Matsumura P., Adler J. Use of a computer to assay motility in bacteria. Anal Biochem. 1988 Sep;173(2):271–277. doi: 10.1016/0003-2697(88)90189-3. [DOI] [PubMed] [Google Scholar]
  18. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
  19. Sundberg S. A., Alam M., Spudich J. L. Excitation signal processing times in Halobacterium halobium phototaxis. Biophys J. 1986 Nov;50(5):895–900. doi: 10.1016/S0006-3495(86)83530-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Taylor B. L., Koshland D. E., Jr Reversal of flagellar rotation in monotrichous and peritrichous bacteria: generation of changes in direction. J Bacteriol. 1974 Aug;119(2):640–642. doi: 10.1128/jb.119.2.640-642.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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