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. 1989 Aug;171(8):4521–4524. doi: 10.1128/jb.171.8.4521-4524.1989

Sporulation of Myxococcus xanthus in liquid shake flask cultures.

A Rosenbluh 1, E Rosenberg 1
PMCID: PMC210238  PMID: 2502539

Abstract

When suspended in a liquid starvation medium, exponentially growing Myxococcus xanthus sporulated within 3 days. These myxospores were similar to spores developed within fruiting bodies, as determined by electron microscopy and the production of spore-specific protein S. This liquid sporulation system may be useful as a means of preparing large quantities of myxospores and extracellular fluid for biochemical studies, including isolation of chemical signals produced during the sporulation process.

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Selected References

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