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. 1989 Oct;171(10):5467–5472. doi: 10.1128/jb.171.10.5467-5472.1989

Molecular relationship of chromosomal genes encoding biphenyl/polychlorinated biphenyl catabolism: some soil bacteria possess a highly conserved bph operon.

K Furukawa 1, N Hayase 1, K Taira 1, N Tomizuka 1
PMCID: PMC210385  PMID: 2507526

Abstract

All the genes we examined that encoded biphenyl/polychlorinated biphenyl (PCB) degradation were chromosomal, unlike many other degradation-encoding genes, which are plasmid borne. The molecular relationship of genes coding for biphenyl/PCB catabolism in various biphenyl/PCB-degrading Pseudomonas, Achromobacter, Alcaligenes, Moraxella, and Arthrobacter strains was investigated. Among 15 strains tested, 5 Pseudomonas strains and one Alcaligenes strain possessed the bphABC gene cluster on the XhoI 7.2-kilobase fragment corresponding to that of Pseudomonas pseudoalcaligenes KF707. More importantly, the restriction profiles of these XhoI 7.2-kilobase fragments containing bphABC genes were very similar, if not identical, despite the dissimilarity of the flanking chromosomal regions. Three other strains also possessed bphABC genes homologous with those of KF707, and five other strains showed weak or no significant genetic homology with bphABC of KF707. The immunological cross-reactivity of 2,3-dihydroxybiphenyl dioxygenases from various strains corresponded well to the DNA homology. On the other hand, the bphC gene of another PCB-degrading strain, Pseudomonas paucimobilis Q1, lacked genetic as well as immunological homology with any of the other 15 biphenyl/PCB degraders tested. The existence of the nearly identical chromosomal genes among various strains may suggest that a segment containing the bphABC genes has a mechanism for transferring the gene from one strain to another.

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Selected References

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  1. Ahmed M., Focht D. D. Degradation of polychlorinated biphenyls by two species of Achromobacter. Can J Microbiol. 1973 Jan;19(1):47–52. doi: 10.1139/m73-007. [DOI] [PubMed] [Google Scholar]
  2. Bedard D. L., Unterman R., Bopp L. H., Brennan M. J., Haberl M. L., Johnson C. Rapid assay for screening and characterizing microorganisms for the ability to degrade polychlorinated biphenyls. Appl Environ Microbiol. 1986 Apr;51(4):761–768. doi: 10.1128/aem.51.4.761-768.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bedard D. L., Wagner R. E., Brennan M. J., Haberl M. L., Brown J. F., Jr Extensive degradation of Aroclors and environmentally transformed polychlorinated biphenyls by Alcaligenes eutrophus H850. Appl Environ Microbiol. 1987 May;53(5):1094–1102. doi: 10.1128/aem.53.5.1094-1102.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Furukawa K., Arimura N. Purification and properties of 2,3-dihydroxybiphenyl dioxygenase from polychlorinated biphenyl-degrading Pseudomonas pseudoalcaligenes and Pseudomonas aeruginosa carrying the cloned bphC gene. J Bacteriol. 1987 Feb;169(2):924–927. doi: 10.1128/jb.169.2.924-927.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Furukawa K., Chakrabarty A. M. Involvement of plasmids in total degradation of chlorinated biphenyls. Appl Environ Microbiol. 1982 Sep;44(3):619–626. doi: 10.1128/aem.44.3.619-626.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Furukawa K., Miyazaki T. Cloning of a gene cluster encoding biphenyl and chlorobiphenyl degradation in Pseudomonas pseudoalcaligenes. J Bacteriol. 1986 May;166(2):392–398. doi: 10.1128/jb.166.2.392-398.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Furukawa K., Miyazaki T., Tomizuka N. SAL-TOL in vivo recombinant plasmid pKF439. J Bacteriol. 1985 Jun;162(3):1325–1328. doi: 10.1128/jb.162.3.1325-1328.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Furukawa K., Simon J. R., Chakrabarty A. M. Common induction and regulation of biphenyl, xylene/toluene, and salicylate catabolism in Pseudomonas paucimobilis. J Bacteriol. 1983 Jun;154(3):1356–1362. doi: 10.1128/jb.154.3.1356-1362.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Keil H., Keil S., Pickup R. W., Williams P. A. Evolutionary conservation of genes coding for meta pathway enzymes within TOL plasmids pWW0 and pWW53. J Bacteriol. 1985 Nov;164(2):887–895. doi: 10.1128/jb.164.2.887-895.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kutsuna M., Someda K., Morita K., Yamanouchi Y., Kurimoto T., Kawamura Y., Matsumura H. [Ischemic cerebral symptoms after subarachnoid hemorrhage due to aneurysmal rupture (author's transl)]. No Shinkei Geka. 1978 Jun;6(6):543–548. [PubMed] [Google Scholar]
  12. Lehrbach P. R., McGregor I., Ward J. M., Broda P. Molecular relationships between pseudomonas INC P-9 degradative plasmids TOL, NAH, and SAL. Plasmid. 1983 Sep;10(2):164–174. doi: 10.1016/0147-619x(83)90069-0. [DOI] [PubMed] [Google Scholar]
  13. Lehrbach P. R., Ward J., Meulien P., Broda P. Physical mapping of TOL plasmids pWWO and pND2 and various R plasmid-TOL derivatives from Pseudomonas spp. J Bacteriol. 1982 Dec;152(3):1280–1283. doi: 10.1128/jb.152.3.1280-1283.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mondello F. J. Cloning and expression in Escherichia coli of Pseudomonas strain LB400 genes encoding polychlorinated biphenyl degradation. J Bacteriol. 1989 Mar;171(3):1725–1732. doi: 10.1128/jb.171.3.1725-1732.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ruisinger S., Klages U., Lingens F. Abbau der 4-chlorbenzoesäure durch eine Arthrobacter-Species. Arch Microbiol. 1976 Nov 2;110(23):253–256. doi: 10.1007/BF00690235. [DOI] [PubMed] [Google Scholar]
  16. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  17. Taira K., Hayase N., Arimura N., Yamashita S., Miyazaki T., Furukawa K. Cloning and nucleotide sequence of the 2,3-dihydroxybiphenyl dioxygenase gene from the PCB-degrading strain of Pseudomonas paucimobilis Q1. Biochemistry. 1988 May 31;27(11):3990–3996. doi: 10.1021/bi00411a015. [DOI] [PubMed] [Google Scholar]
  18. Tsuda M., Iino T. Genetic analysis of a transposon carrying toluene degrading genes on a TOL plasmid pWW0. Mol Gen Genet. 1987 Dec;210(2):270–276. doi: 10.1007/BF00325693. [DOI] [PubMed] [Google Scholar]
  19. Tsuda M., Iino T. Identification and characterization of Tn4653, a transposon covering the toluene transposon Tn4651 on TOL plasmid pWW0. Mol Gen Genet. 1988 Jul;213(1):72–77. doi: 10.1007/BF00333400. [DOI] [PubMed] [Google Scholar]
  20. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
  21. Yates J. R., Mondello F. J. Sequence similarities in the genes encoding polychlorinated biphenyl degradation by Pseudomonas strain LB400 and Alcaligenes eutrophus H850. J Bacteriol. 1989 Mar;171(3):1733–1735. doi: 10.1128/jb.171.3.1733-1735.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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