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. 1989 Nov;171(11):5987–5994. doi: 10.1128/jb.171.11.5987-5994.1989

The tyrT locus of Escherichia coli exhibits a regulatory function for glycine metabolism.

U Michelsen 1, M Bösl 1, T Dingermann 1, H Kersten 1
PMCID: PMC210463  PMID: 2681148

Abstract

The tyrT locus in Escherichia coli codes for two gene copies of tRNA(1Tyr). Both genes are organized in one operon, which has a unique structure. The two tRNA genes are separated by a spacer segment highly homologous to a part of a unit which is repeated three times in the distal portion of the locus. This operon also contains coding capacity for a small basic protein. A genomic deletion of this locus was constructed and marked by a kanamycin resistance cassette. Deletion mutants exhibited a characteristic phenotype when cells were shifted from rich medium to minimal medium. The cells entered a transient lag phase, apparently resulting from specific glycine starvation. This phenotype involved stringent response and was therefore not observed in relA derivatives. The genomic deletion was complemented in trans by a plasmid-borne tyrT locus. From deletion mapping, it can be concluded that a product of the tyrT operon is responsible for complementation. However, neither the tRNA(1Tyr) nor the proposed basic protein is the complementation-competent entity.

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Selected References

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  1. Altman S., Model P., Dixon G. H., Wosnick N. A. An E. coli gene coding for a protamine-like protein. Cell. 1981 Nov;26(3 Pt 1):299–304. doi: 10.1016/0092-8674(81)90198-7. [DOI] [PubMed] [Google Scholar]
  2. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Casadaban M. J., Cohen S. N. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530–4533. doi: 10.1073/pnas.76.9.4530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cashel M., Gallant J. Control of RNA synthesis in Escherichia coli. I. Amino acid dependence of the synthesis of the substrates of RNA polymerase. J Mol Biol. 1968 Jul 14;34(2):317–330. doi: 10.1016/0022-2836(68)90256-8. [DOI] [PubMed] [Google Scholar]
  5. Cashel M., Gallant J. Two compounds implicated in the function of the RC gene of Escherichia coli. Nature. 1969 Mar 1;221(5183):838–841. doi: 10.1038/221838a0. [DOI] [PubMed] [Google Scholar]
  6. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Drew H. R., Travers A. A. DNA structural variations in the E. coli tyrT promoter. Cell. 1984 Jun;37(2):491–502. doi: 10.1016/0092-8674(84)90379-9. [DOI] [PubMed] [Google Scholar]
  8. Egan J., Landy A. Structural analysis of the tRNA1Tyr gene of Escherichia coli. A 178 base pair sequence that is repeated 3.14 times. J Biol Chem. 1978 May 25;253(10):3607–3622. [PubMed] [Google Scholar]
  9. Fraser J., Newman E. B. Derivation of glycine from threonine in Escherichia coli K-12 mutants. J Bacteriol. 1975 Jun;122(3):810–817. doi: 10.1128/jb.122.3.810-817.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gross H. J., Raab C. In vivo synthesis of tRNA Tyr 1 and tRNA Tyr 2 : differences in "early" and "late log" E. coli MRE 600. Biochem Biophys Res Commun. 1972 Mar 24;46(6):2006–2011. doi: 10.1016/0006-291x(72)90751-6. [DOI] [PubMed] [Google Scholar]
  11. Hudson L., Rossi J., Landy A. Dual function transcripts specifying tRNA and mRNA. Nature. 1981 Dec 3;294(5840):422–427. doi: 10.1038/294422a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kuchino Y., Mori F., Nishimura S. Structure and transcription of the tRNAPro1 gene from Escherichia coli. Nucleic Acids Res. 1985 May 10;13(9):3213–3220. doi: 10.1093/nar/13.9.3213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lamond A. I., Travers A. A. Genetically separable functional elements mediate the optimal expression and stringent regulation of a bacterial tRNA gene. Cell. 1985 Feb;40(2):319–326. doi: 10.1016/0092-8674(85)90146-1. [DOI] [PubMed] [Google Scholar]
  14. Lau P. P., Gray H. B., Jr Extracellular nucleases of Alteromonas espejiana BAL 31.IV. The single strand-specific deoxyriboendonuclease activity as a probe for regions of altered secondary structure in negatively and positively supercoiled closed circular DNA. Nucleic Acids Res. 1979 Jan;6(1):331–357. doi: 10.1093/nar/6.1.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  16. McCorkle G. M., Altman S. Large deletion mutants of Escherichia coli tRNATyr1. J Mol Biol. 1982 Feb 25;155(2):83–103. doi: 10.1016/0022-2836(82)90438-7. [DOI] [PubMed] [Google Scholar]
  17. Reed R. E., Altman S. Repeated sequences and open reading frames in the 3' flanking region of the gene for the RNA subunit of Escherichia coli ribonuclease P. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5359–5363. doi: 10.1073/pnas.80.17.5359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rossi J., Egan J., Hudson L., Landy A. The tyrT locus: termination and processing of a complex transcript. Cell. 1981 Nov;26(3 Pt 1):305–314. doi: 10.1016/0092-8674(81)90199-9. [DOI] [PubMed] [Google Scholar]
  19. Russell R. L., Abelson J. N., Landy A., Gefter M. L., Brenner S., Smith J. D. Duplicate genes for tyrosine transfer RNA in Escherichia coli. J Mol Biol. 1970 Jan 14;47(1):1–13. doi: 10.1016/0022-2836(70)90397-9. [DOI] [PubMed] [Google Scholar]
  20. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shand R. F., Blum P. H., Mueller R. D., Riggs D. L., Artz S. W. Correlation between histidine operon expression and guanosine 5'-diphosphate-3'-diphosphate levels during amino acid downshift in stringent and relaxed strains of Salmonella typhimurium. J Bacteriol. 1989 Feb;171(2):737–743. doi: 10.1128/jb.171.2.737-743.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sprinzl M., Hartmann T., Meissner F., Moll J., Vorderwülbecke T. Compilation of tRNA sequences and sequences of tRNA genes. Nucleic Acids Res. 1987;15 (Suppl):r53–188. doi: 10.1093/nar/15.suppl.r53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Travers A. A., Lamond A. I., Mace H. A., Berman M. L. RNA polymerase interactions with the upstream region of the E. coli tyrT promoter. Cell. 1983 Nov;35(1):265–273. doi: 10.1016/0092-8674(83)90229-5. [DOI] [PubMed] [Google Scholar]
  24. Travers A. A., Lamond A. I., Weeks J. R. Alteration of the growth-rate-dependent regulation of Escherichia coli tyrT expression by promoter mutations. J Mol Biol. 1986 May 5;189(1):251–255. doi: 10.1016/0022-2836(86)90397-9. [DOI] [PubMed] [Google Scholar]
  25. Winans S. C., Elledge S. J., Krueger J. H., Walker G. C. Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli. J Bacteriol. 1985 Mar;161(3):1219–1221. doi: 10.1128/jb.161.3.1219-1221.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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