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. 1989 Dec;171(12):6845–6849. doi: 10.1128/jb.171.12.6845-6849.1989

The Agrobacterium tumefaciens virC1 gene product binds to overdrive, a T-DNA transfer enhancer.

N Toro 1, A Datta 1, O A Carmi 1, C Young 1, R K Prusti 1, E W Nester 1
PMCID: PMC210585  PMID: 2592351

Abstract

In Agrobacterium tumefaciens, a cis-active 24-base-pair sequence adjacent to the right border of the T-DNA, called overdrive, stimulates tumor formation by increasing the level of T-DNA processing. Recent results from our laboratory have suggested that the virC operon which enhances T-DNA processing probably does so because the VirC1 protein interacts with overdrive (N. Toro, A. Datta, M. Yanofsky, and E. W. Nester, Proc. Natl. Acad. Sci. USA 85:8558-8562, 1988). We report here the purification of the VirC1 protein from cells of Escherichia coli harboring a plasmid containing the coding sequences of the virC locus of the octopine Ti plasmid. By gel mobility shift and DNase I footprinting assays, we showed that this purified virC1 gene product binds to overdrive but not to the right border of T-DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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