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. 1989 Dec;171(12):6867–6869. doi: 10.1128/jb.171.12.6867-6869.1989

Suppression of the lethal effect of acidic-phospholipid deficiency by defective formation of the major outer membrane lipoprotein in Escherichia coli.

Y Asai 1, Y Katayose 1, C Hikita 1, A Ohta 1, I Shibuya 1
PMCID: PMC210590  PMID: 2556377

Abstract

The Escherichia coli pgsA3 allele encoding a defective phosphatidylglycerophosphate synthase is lethal for all but certain strains. Genetic analysis of such strains has revealed that the lethal effect is fully suppressed by the lack of the major outer membrane lipoprotein that consumes phosphatidylglycerol for its maturation.

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Selected References

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  1. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braun V. Covalent lipoprotein from the outer membrane of Escherichia coli. Biochim Biophys Acta. 1975 Oct 31;415(3):335–377. doi: 10.1016/0304-4157(75)90013-1. [DOI] [PubMed] [Google Scholar]
  3. Braun V., Rehn K. Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli cell wall. The specific effect of trypsin on the membrane structure. Eur J Biochem. 1969 Oct;10(3):426–438. doi: 10.1111/j.1432-1033.1969.tb00707.x. [DOI] [PubMed] [Google Scholar]
  4. Chattopadhyay P. K., Wu H. C. Biosynthesis of the covalently linked diglyceride in murein lipoprotein of Escherichia coli. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5318–5322. doi: 10.1073/pnas.74.12.5318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chung C. T., Niemela S. L., Miller R. H. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2172–2175. doi: 10.1073/pnas.86.7.2172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heacock P. N., Dowhan W. Construction of a lethal mutation in the synthesis of the major acidic phospholipids of Escherichia coli. J Biol Chem. 1987 Sep 25;262(27):13044–13049. [PubMed] [Google Scholar]
  7. Hirota Y., Suzuki H., Nishimura Y., Yasuda S. On the process of cellular division in Escherichia coli: a mutant of E. coli lacking a murein-lipoprotein. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1417–1420. doi: 10.1073/pnas.74.4.1417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hussain M., Ichihara S., Mizushima S. Accumulation of glyceride-containing precursor of the outer membrane lipoprotein in the cytoplasmic membrane of Escherichia coli treated with globomycin. J Biol Chem. 1980 Apr 25;255(8):3707–3712. [PubMed] [Google Scholar]
  9. Hussain M., Ichihara S., Mizushima S. Mechanism of signal peptide cleavage in the biosynthesis of the major lipoprotein of the Escherichia coli outer membrane. J Biol Chem. 1982 May 10;257(9):5177–5182. [PubMed] [Google Scholar]
  10. Inouye M., Shaw J., Shen C. The assembly of a structural lipoprotein in the envelope of Escherichia coli. J Biol Chem. 1972 Dec 25;247(24):8154–8159. [PubMed] [Google Scholar]
  11. Kennedy E. P. Osmotic regulation and the biosynthesis of membrane-derived oligosaccharides in Escherichia coli. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1092–1095. doi: 10.1073/pnas.79.4.1092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kobayashi T., Ohta A., Shibuya I. Membrane phospholipid synthesis in Escherichia coli: alteration by glycerol and physiological consequences in a pss mutant. J Biochem. 1986 May;99(5):1393–1400. doi: 10.1093/oxfordjournals.jbchem.a135608. [DOI] [PubMed] [Google Scholar]
  13. Lai J. S., Philbrick W. M., Hayashi S., Inukai M., Arai M., Hirota Y., Wu H. C. Globomycin sensitivity of Escherichia coli and Salmonella typhimurium: effects of mutations affecting structures of murein lipoprotein. J Bacteriol. 1981 Jan;145(1):657–660. doi: 10.1128/jb.145.1.657-660.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lee N., Nakamura K., Inouye M. Expression of the Serratia marcescens lipoproteins gene in Escherichia coli. J Bacteriol. 1981 Jun;146(3):861–866. doi: 10.1128/jb.146.3.861-866.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lin J. J., Kanazawa H., Ozols J., Wu H. C. An Escherichia coli mutant with an amino acid alteration within the signal sequence of outer membrane prolipoprotein. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4891–4895. doi: 10.1073/pnas.75.10.4891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lin J. J., Kanazawa H., Wu H. C. Assembly of outer membrane lipoprotein in an Escherichia coli mutant with a single amino acid replacement within the signal sequence of prolipoprotein. J Bacteriol. 1980 Feb;141(2):550–557. doi: 10.1128/jb.141.2.550-557.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Miyazaki C., Kuroda M., Ohta A., Shibuya I. Genetic manipulation of membrane phospholipid composition in Escherichia coli: pgsA mutants defective in phosphatidylglycerol synthesis. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7530–7534. doi: 10.1073/pnas.82.22.7530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nishijima S., Asami Y., Uetake N., Yamagoe S., Ohta A., Shibuya I. Disruption of the Escherichia coli cls gene responsible for cardiolipin synthesis. J Bacteriol. 1988 Feb;170(2):775–780. doi: 10.1128/jb.170.2.775-780.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ohta A., Shibuya I. Membrane phospholipid synthesis and phenotypic correlation of an Escherichia coli pss mutant. J Bacteriol. 1977 Nov;132(2):434–443. doi: 10.1128/jb.132.2.434-443.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ohta A., Waggoner K., Radominska-Pyrek A., Dowhan W. Cloning of genes involved in membrane lipid synthesis: effects of amplification of phosphatidylglycerophosphate synthase in Escherichia coli. J Bacteriol. 1981 Aug;147(2):552–562. doi: 10.1128/jb.147.2.552-562.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Raetz C. R. Isolation of Escherichia coli mutants defective in enzymes of membrane lipid synthesis. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2274–2278. doi: 10.1073/pnas.72.6.2274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schnaitman C. A. Effect of ethylenediaminetetraacetic acid, Triton X-100, and lysozyme on the morphology and chemical composition of isolate cell walls of Escherichia coli. J Bacteriol. 1971 Oct;108(1):553–563. doi: 10.1128/jb.108.1.553-563.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shibuya I., Miyazaki C., Ohta A. Alteration of phospholipid composition by combined defects in phosphatidylserine and cardiolipin synthases and physiological consequences in Escherichia coli. J Bacteriol. 1985 Mar;161(3):1086–1092. doi: 10.1128/jb.161.3.1086-1092.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yem D. W., Wu H. C. Physiological characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein. J Bacteriol. 1978 Mar;133(3):1419–1426. doi: 10.1128/jb.133.3.1419-1426.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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