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. 1988 Jan;170(1):463–467. doi: 10.1128/jb.170.1.463-467.1988

Genetic and molecular characterization of the guaC-nadC-aroP region of Escherichia coli K-12.

R E Roberts 1, C I Lienhard 1, C G Gaines 1, J M Smith 1, J R Guest 1
PMCID: PMC210669  PMID: 3275629

Abstract

The guaC (GMP reductase), nadC (quinolinate phosphoribosyltransferase), and aroP (aromatic amino acid permease) genes of Escherichia coli K-12 were located in the 2.5-min region of the chromosome (muT-guaC-nadC-aroP-aceE) by a combination of linkage analysis, deletion mapping, restriction analysis, and plasmid subcloning. The guaC locus expressed a product of Mr 37,000 with a clockwise transcriptional polarity, and the GMP reductase activities of guaC+ plasmid-containing strains were amplified 15- to 20-fold.

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Selected References

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