Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Feb;170(2):995–997. doi: 10.1128/jb.170.2.995-997.1988

Distribution of 10-formyltetrahydrofolate synthetase in eubacteria.

T R Whitehead 1, M Park 1, J C Rabinowitz 1
PMCID: PMC210755  PMID: 3257484

Abstract

The distribution of 10-formyltetrahydrofolate synthetase, which activates formate for use as a one-carbon donor in a variety of biosynthetic reactions, was determined for a variety of eubacteria. Organisms from several genera were found to lack detectable synthetase activity; however, all organisms tested were found to contain 5,10-methylenetetrahydrofolate dehydrogenase activity.

Full text

PDF
997

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams J. M., Capecchi M. R. N-formylmethionyl-sRNA as the initiator of protein synthesis. Proc Natl Acad Sci U S A. 1966 Jan;55(1):147–155. doi: 10.1073/pnas.55.1.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Caperelli C. A., Benkovic P. A., Chettur G., Benkovic S. J. Purification of a complex catalyzing folate cofactor synthesis and transformylation in de novo purine biosynthesis. J Biol Chem. 1980 Mar 10;255(5):1885–1890. [PubMed] [Google Scholar]
  3. Delk A. S., Nagle D. P., Jr, Rabinowitz J. C. Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2. J Biol Chem. 1980 May 25;255(10):4387–4390. [PubMed] [Google Scholar]
  4. Dev I. K., Harvey R. J. A complex of N5,N10-methylenetetrahydrofolate dehydrogenase and N5,N10-methenyltetrahydrofolate cyclohydrolase in Escherichia coli. Purification, subunit structure, and allosteric inhibition by N10-formyltetrahydrofolate. J Biol Chem. 1978 Jun 25;253(12):4245–4253. [PubMed] [Google Scholar]
  5. Dev I. K., Harvey R. J. Sources of one-carbon units in the folate pathway of Escherichia coli. J Biol Chem. 1982 Feb 25;257(4):1980–1986. [PubMed] [Google Scholar]
  6. Fox G. E., Stackebrandt E., Hespell R. B., Gibson J., Maniloff J., Dyer T. A., Wolfe R. S., Balch W. E., Tanner R. S., Magrum L. J. The phylogeny of prokaryotes. Science. 1980 Jul 25;209(4455):457–463. doi: 10.1126/science.6771870. [DOI] [PubMed] [Google Scholar]
  7. Gray G. R., Ballou C. E. Methylated polysaccharide activators of fatty acid synthase from Mycobacterium phlei. Methods Enzymol. 1975;35:90–95. doi: 10.1016/0076-6879(75)35142-2. [DOI] [PubMed] [Google Scholar]
  8. Himes R. H., Harmony J. A. Formyltetrahydrofolate synthetase. CRC Crit Rev Biochem. 1973 Sep;1(4):501–535. doi: 10.3109/10409237309105441. [DOI] [PubMed] [Google Scholar]
  9. MARCKER K., SANGER F. N-FORMYL-METHIONYL-S-RNA. J Mol Biol. 1964 Jun;8:835–840. doi: 10.1016/s0022-2836(64)80164-9. [DOI] [PubMed] [Google Scholar]
  10. McGuire J. J., Rabinowitz J. C. Studies on the mechanism of formyltetrahydrofolate synthetase. The Peptococcus aerogenes enzyme. J Biol Chem. 1978 Feb 25;253(4):1079–1085. [PubMed] [Google Scholar]
  11. O'Brien W. E., Brewer J. M., Ljungdahl L. G. Purification and characterization of thermostable 5,10-methylenetetrahydrofolate dehydrogenase from Clostridium thermoaceticum. J Biol Chem. 1973 Jan 25;248(2):403–408. [PubMed] [Google Scholar]
  12. Paukert J. L., Straus L. D., Rabinowitz J. C. Formyl-methyl-methylenetetrahydrofolate synthetase-(combined). An ovine protein with multiple catalytic activities. J Biol Chem. 1976 Aug 25;251(16):5104–5111. [PubMed] [Google Scholar]
  13. Schirch L. Formyl-methenyl-methylenetetrahydrofolate synthetase from rabbit liver (combined). Evidence for a single site in the conversion of 5,10-methylenetetrahydrofolate to 10-formyltetrahydrofolate. Arch Biochem Biophys. 1978 Aug;189(2):283–290. doi: 10.1016/0003-9861(78)90214-x. [DOI] [PubMed] [Google Scholar]
  14. Shannon K. W., Rabinowitz J. C. Purification and characterization of a mitochondrial isozyme of C1-tetrahydrofolate synthase from Saccharomyces cerevisiae. J Biol Chem. 1986 Sep 15;261(26):12266–12271. [PubMed] [Google Scholar]
  15. Staben C., Rabinowitz J. C. Immunological crossreactivity of eukaryotic C1-tetrahydrofolate synthase and prokaryotic 10-formyltetrahydrofolate synthetase. Proc Natl Acad Sci U S A. 1983 Nov;80(22):6799–6803. doi: 10.1073/pnas.80.22.6799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Staben C., Rabinowitz J. C. Nucleotide sequence of the Saccharomyces cerevisiae ADE3 gene encoding C1-tetrahydrofolate synthase. J Biol Chem. 1986 Apr 5;261(10):4629–4637. [PubMed] [Google Scholar]
  17. Staben C., Whitehead T. R., Rabinowitz J. C. Heparin-agarose chromatography for the purification of tetrahydrofolate utilizing enzymes: C1-tetrahydrofolate synthase and 10-formyltetrahydrofolate synthetase. Anal Biochem. 1987 Apr;162(1):257–264. doi: 10.1016/0003-2697(87)90035-2. [DOI] [PubMed] [Google Scholar]
  18. Tan L. U., Drury E. J., MacKenzie R. E. Methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase. A multifunctional protein from porcine liver. J Biol Chem. 1977 Feb 10;252(3):1117–1122. [PubMed] [Google Scholar]
  19. WHITELEY H. R., OSBORN M. J., HUENNEKENS F. M. Purification and properties of the formate-activating enzyme from Micrococcus aerogenes. J Biol Chem. 1959 Jun;234(6):1538–1543. [PubMed] [Google Scholar]
  20. Whitehead T. R., Rabinowitz J. C. Cloning and expression in Escherichia coli of the gene for 10-formyltetrahydrofolate synthetase from Clostridium acidiurici ("Clostridium acidi-urici"). J Bacteriol. 1986 Jul;167(1):205–209. doi: 10.1128/jb.167.1.205-209.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES