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. 1988 Feb;170(2):1021–1025. doi: 10.1128/jb.170.2.1021-1025.1988

Cloning of the Escherichia coli K-12 hemB gene.

J M Li 1, H Umanoff 1, R Proenca 1, C S Russell 1, S D Cosloy 1
PMCID: PMC210762  PMID: 3276659

Abstract

An Escherichia coli heme-requiring, heme-permeable mutant had no detectable 5-aminolevulinate dehydratase or porphobilinogen deaminase activities. The gene which complemented this mutation was cloned to a high-copy-number plasmid, and porphobilinogen deaminase activity was restored to normal levels, but the synthesis of 5-aminolevulinate dehydratase increased 20- to 30-fold. A maxicell procedure confirmed that the gene cloned was hemB.

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Selected References

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