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. 1988 Mar;170(3):1153–1161. doi: 10.1128/jb.170.3.1153-1161.1988

A plasmid of Rhizobium meliloti 41 encodes catabolism of two compounds from root exudate of Calystegium sepium.

D Tepfer 1, A Goldmann 1, N Pamboukdjian 1, M Maille 1, A Lepingle 1, D Chevalier 1, J Dénarié 1, C Rosenberg 1
PMCID: PMC210886  PMID: 2981046

Abstract

Our objectives were to identify substances produced by plant roots that might act as nutritional mediators of specific plant-bacterium relationships and to delineate the bacterial genes responsible for catabolizing these substances. We discovered new compounds, which we call calystegins, that have the characteristics of nutritional mediators. They were detected in only 3 of 105 species of higher plants examined: Calystegia sepium, Convolvulus arvensis (both of the Convolvulaceae family), and Atropa belladonna. Calystegins are abundant in organs in contact with the rhizosphere and are not found, or are observed only in small quantities, in aerial plant parts. Just as the synthesis of calystegins is infrequent in the plant kingdom, their catabolism is rare among rhizosphere bacteria that associate with plants and influence their growth. Of 42 such bacteria tested, only one (Rhizobium meliloti 41) was able to catabolize calystegins and use them as a sole source of carbon and nitrogen. The calystegin catabolism gene(s) (cac) in this strain is located on a self-transmissible plasmid (pRme41a), which is not essential to nitrogen-fixing symbiosis with legumes. We suggest that under natural conditions calystegins provide an exclusive carbon and nitrogen source to rhizosphere bacteria which are able to catabolize these compounds. Calystegins (and the corresponding microbial catabolic genes) might be used to analyze and possibly modify rhizosphere ecology.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banfalvi Z., Kondorosi E., Kondorosi A. Rhizobium meliloti carries two megaplasmids. Plasmid. 1985 Mar;13(2):129–138. doi: 10.1016/0147-619x(85)90065-4. [DOI] [PubMed] [Google Scholar]
  2. Boucher C., Bergeron B., De Bertalmio M. B., Dénarié J. Introduction of bacteriophage Mu into Pseudomonas solanacearum and Rhizobium meliloti using the R factor RP4. J Gen Microbiol. 1977 Jan;98(1):253–263. doi: 10.1099/00221287-98-1-253. [DOI] [PubMed] [Google Scholar]
  3. Bouzar H., Moore L. W. Isolation of different agrobacterium biovars from a natural oak savanna and tallgrass prairie. Appl Environ Microbiol. 1987 Apr;53(4):717–721. doi: 10.1128/aem.53.4.717-721.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bánfalvi Z., Sakanyan V., Koncz C., Kiss A., Dusha I., Kondorosi A. Location of nodulation and nitrogen fixation genes on a high molecular weight plasmid of R. meliloti. Mol Gen Genet. 1981;184(2):318–325. doi: 10.1007/BF00272925. [DOI] [PubMed] [Google Scholar]
  5. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. Properties of an R factor from Pseudomonas aeruginosa. J Bacteriol. 1971 Dec;108(3):1244–1249. doi: 10.1128/jb.108.3.1244-1249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guyon P., Chilton M. D., Petit A., Tempé J. Agropine in "null-type" crown gall tumors: Evidence for generality of the opine concept. Proc Natl Acad Sci U S A. 1980 May;77(5):2693–2697. doi: 10.1073/pnas.77.5.2693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Murphy P. J., Heycke N., Banfalvi Z., Tate M. E., de Bruijn F., Kondorosi A., Tempé J., Schell J. Genes for the catabolism and synthesis of an opine-like compound in Rhizobium meliloti are closely linked and on the Sym plasmid. Proc Natl Acad Sci U S A. 1987 Jan;84(2):493–497. doi: 10.1073/pnas.84.2.493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Peters N. K., Frost J. W., Long S. R. A plant flavone, luteolin, induces expression of Rhizobium meliloti nodulation genes. Science. 1986 Aug 29;233(4767):977–980. doi: 10.1126/science.3738520. [DOI] [PubMed] [Google Scholar]
  9. Rosenberg C., Boistard P., Dénarié J., Casse-Delbart F. Genes controlling early and late functions in symbiosis are located on a megaplasmid in Rhizobium meliloti. Mol Gen Genet. 1981;184(2):326–333. doi: 10.1007/BF00272926. [DOI] [PubMed] [Google Scholar]
  10. Rosenberg C., Casse-Delbart F., Dusha I., David M., Boucher C. Megaplasmids in the plant-associated bacteria Rhizobium meliloti and Pseudomonas solanacearum. J Bacteriol. 1982 Apr;150(1):402–406. doi: 10.1128/jb.150.1.402-406.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ruvkun G. B., Ausubel F. M. A general method for site-directed mutagenesis in prokaryotes. Nature. 1981 Jan 1;289(5793):85–88. doi: 10.1038/289085a0. [DOI] [PubMed] [Google Scholar]
  12. Schell J., Van Montagu M., De Beuckeleer M., De Block M., Depicker A., De Wilde M., Engler G., Genetello C., Hernalsteens J. P., Holsters M. Interactions and DNA transfer between Agrobacterium tumefaciens, the Ti-plasmid and the plant host. Proc R Soc Lond B Biol Sci. 1979 Apr 11;204(1155):251–266. doi: 10.1098/rspb.1979.0026. [DOI] [PubMed] [Google Scholar]
  13. Stanisich V. A. The properties and host range of male-specific bacteriophages of Pseudomonas aeruginosa. J Gen Microbiol. 1974 Oct;84(2):332–342. doi: 10.1099/00221287-84-2-332. [DOI] [PubMed] [Google Scholar]
  14. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  15. Tepfer D. Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell. 1984 Jul;37(3):959–967. doi: 10.1016/0092-8674(84)90430-6. [DOI] [PubMed] [Google Scholar]
  16. Truchet G., Rosenberg C., Vasse J., Julliot J. S., Camut S., Denarie J. Transfer of Rhizobium meliloti pSym genes into Agrobacterium tumefaciens: host-specific nodulation by atypical infection. J Bacteriol. 1984 Jan;157(1):134–142. doi: 10.1128/jb.157.1.134-142.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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