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. 1973 Nov 1;59(2):471–479. doi: 10.1083/jcb.59.2.471

ANTAGONISM BY DIBUTYRYL ADENOSINE CYCLIC 3',5'-MONOPHOSPHATE AND TESTOSTERONE OF CELL ROUNDING REACTIONS

Brian Storrie 1
PMCID: PMC2109083  PMID: 4375682

Abstract

In an attempt to understand further the mechanism of the morphological and functional "reverse transformation" of CHO-K1 cells induced by dibutyryl adenosine cyclic 3',5'-monophosphate (cAMP) and testosterone, the kinetics of variation in the susceptibility of cells to rounding after the addition or deletion of dibutyryl cAMP and testosterone have been investigated. Changes in susceptibility to cell rounding upon removal of divalent cations or pulse exposure to concanavalin A were complete within 0.5–1 h after addition or deletion of drug. In comparison, the gross conversion of CHO-K1 cells from epithelial- to fibroblast-like morphology after drug treatment or the converse change after drug removal required 8 or 4 h, respectively. The effects on cell rounding are not caused by an effect of dibutyryl cAMP upon cell growth rate. Inhibitor experiments indicate that the changes investigated do not require continued RNA or protein synthesis and are not prevented by agents which depolymerize microtubules.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burger M. M., Bombik B. M., Breckenridge B. M., Sheppard J. R. Growth control and cyclic alterations of cyclic AMP in the cell cycle. Nat New Biol. 1972 Oct 11;239(93):161–163. doi: 10.1038/newbio239161a0. [DOI] [PubMed] [Google Scholar]
  2. Burger M. M., Noonan K. D. Restoration of normal growth by covering of agglutinin sites on tumour cell surface. Nature. 1970 Nov 7;228(5271):512–515. doi: 10.1038/228512a0. [DOI] [PubMed] [Google Scholar]
  3. Burger M. M. Surface changes in transformed cells detected by lectins. Fed Proc. 1973 Jan;32(1):91–101. [PubMed] [Google Scholar]
  4. Eckhart W., Dulbecco R., Burger M. M. Temperature-dependent surface changes in cells infected or transformed by a thermosensitive mutant of polyoma virus. Proc Natl Acad Sci U S A. 1971 Feb;68(2):283–286. doi: 10.1073/pnas.68.2.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fisher H. W., Puck T. T., Sato G. MOLECULAR GROWTH REQUIREMENTS OF SINGLE MAMMALIAN CELLS: THE ACTION OF FETUIN IN PROMOTING CELL ATTACHMENT TO GLASS. Proc Natl Acad Sci U S A. 1958 Jan;44(1):4–10. doi: 10.1073/pnas.44.1.4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fox T. O., Sheppard J. R., Burger M. M. Cyclic membrane changes in animal cells: transformed cells permanently display a surface architecture detected in normal cells only during mitosis. Proc Natl Acad Sci U S A. 1971 Jan;68(1):244–247. doi: 10.1073/pnas.68.1.244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HAM R. G. CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM. Proc Natl Acad Sci U S A. 1965 Feb;53:288–293. doi: 10.1073/pnas.53.2.288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hirano H., Parkhouse B., Nicolson G. L., Lennox E. S., Singer S. J. Distribution of saccharide residues on membrane fragments from a myeloma-cell homogenate: its implications for membrane biogenesis. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2945–2949. doi: 10.1073/pnas.69.10.2945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hsie A. W., Jones C., Puck T. T. Further changes in differentiation state accompanying the conversion of Chinese hamster cells of fibroblastic form by dibutyryl adenosine cyclic 3':5'-monophosphate and hormones. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1648–1652. doi: 10.1073/pnas.68.7.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hsie A. W., Puck T. T. Morphological transformation of Chinese hamster cells by dibutyryl adenosine cyclic 3':5'-monophosphate and testosterone. Proc Natl Acad Sci U S A. 1971 Feb;68(2):358–361. doi: 10.1073/pnas.68.2.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Johnson G. S., Friedman R. M., Pastan I. Restoration of several morphological characteristics of normal fibroblasts in sarcoma cells treated with adenosine-3':5'-cyclic monphosphate and its derivatives. Proc Natl Acad Sci U S A. 1971 Feb;68(2):425–429. doi: 10.1073/pnas.68.2.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kao F. T., Puck T. T. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1275–1281. doi: 10.1073/pnas.60.4.1275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Noonan K. D., Renger H. C., Basilico C., Burger M. M. Surface changes in temperature-sensitive Simian virus 40-transformed cells. Proc Natl Acad Sci U S A. 1973 Feb;70(2):347–349. doi: 10.1073/pnas.70.2.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Otten J., Johnson G. S., Pastan I. Cyclic AMP levels in fibroblasts: relationship to growth rate and contact inhibition of growth. Biochem Biophys Res Commun. 1971 Sep;44(5):1192–1198. doi: 10.1016/s0006-291x(71)80212-7. [DOI] [PubMed] [Google Scholar]
  15. PUCK T. T. STUDIES OF THE LIFE CYCLE OF MAMMALIAN CELLS. Cold Spring Harb Symp Quant Biol. 1964;29:167–176. doi: 10.1101/sqb.1964.029.01.021. [DOI] [PubMed] [Google Scholar]
  16. Patterson D., Waldren C. A. The effect of inhibitors of RNA and protein synthesis on dibutyryl cyclic AMP mediated morphological transformations of Chinese hamster ovary cells in vitro. Biochem Biophys Res Commun. 1973 Jan 23;50(2):566–573. doi: 10.1016/0006-291x(73)90877-2. [DOI] [PubMed] [Google Scholar]
  17. Puck T. T., Waldren C. A., Hsie A. W. Membrane dynamics in the action of dibutyryl adenosine 3':5'-cyclic monophosphate and testosterone on mammalian cells. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1943–1947. doi: 10.1073/pnas.69.7.1943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sanford K. K., Barker B. E., Parshad R., Westfall B. B., Woods M. W., Jackson J. L., King D. R., Peppers E. V. Neoplastic conversion in vitro of mouse cells: cytologic, chromosomal, enzymatic, glycolytic, and growth properties. J Natl Cancer Inst. 1970 Dec;45(6):1071–1096. [PubMed] [Google Scholar]
  19. Sheppard J. R. Difference in the cyclic adenosine 3',5'-monophosphate levels in normal and transformed cells. Nat New Biol. 1972 Mar 1;236(61):14–16. doi: 10.1038/newbio236014a0. [DOI] [PubMed] [Google Scholar]
  20. Sheppard J. R., Levine A. J., Burger M. M. Cell-surface changes after infection with oncogenic viruses: requirement for synthesis of host DNA. Science. 1971 Jun 25;172(3990):1345–1346. doi: 10.1126/science.172.3990.1345. [DOI] [PubMed] [Google Scholar]
  21. Sheppard J. R. Restoration of contact-inhibited growth to transformed cells by dibutyryl adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1316–1320. doi: 10.1073/pnas.68.6.1316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Spudich J. A., Lin S. Cytochalasin B, its interaction with actin and actomyosin from muscle (cell movement-microfilaments-rabbit striated muscle). Proc Natl Acad Sci U S A. 1972 Feb;69(2):442–446. doi: 10.1073/pnas.69.2.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wang J. L., Cunningham B. A., Edelman G. M. Unusual fragments in the subunit structure of concanavalin A. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1130–1134. doi: 10.1073/pnas.68.6.1130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Weisenberg R. C., Borisy G. G., Taylor E. W. The colchicine-binding protein of mammalian brain and its relation to microtubules. Biochemistry. 1968 Dec;7(12):4466–4479. doi: 10.1021/bi00852a043. [DOI] [PubMed] [Google Scholar]
  25. Wright J. A. Evidence for pleiotropic changes in lines of Chinese hamster ovary cells resistant to concanavalin A and phytohemagglutinin-P. J Cell Biol. 1973 Mar;56(3):666–675. doi: 10.1083/jcb.56.3.666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. de Petris S., Raff M. C. Normal distribution, patching and capping of lymphocyte surface immunoglobulin studied by electron microscopy. Nat New Biol. 1973 Feb 28;241(113):257–259. doi: 10.1038/newbio241257a0. [DOI] [PubMed] [Google Scholar]

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