Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Mar;170(3):1350–1353. doi: 10.1128/jb.170.3.1350-1353.1988

Analysis of the variable endpoints generated by one-ended transposition of Tn21.

P Avila 1, J Grinsted 1, F de la Cruz 1
PMCID: PMC210914  PMID: 2830239

Abstract

One-ended transposition of Tn21 generates recombinants usually containing a whole copy of the donor replicon plus a short duplication of it (S. Mötsch, R. Schmitt, P. Avila, F. de la Crue, E. Ward, and J. Grinsted, Nucleic Acids Res. 13:3335-3342, 1985). This work shows that recombinants containing less than a whole copy of the donor replicon (hereafter called short recombinants) could also be detected when plasmid donors which contained two selectable genetic markers were used. Short recombinants were produced at the same frequency from TnpR+ donor molecules as from TnpR- donor molecules in a RecA- background. Therefore, they were not resolution products of larger recombinants. This result invalidates a previous hypothesis to explain one-ended transposition, that is, that one-ended transposition arises from the use of secondary ends by the transposition apparatus. On the other hand, it suggests that one-ended transposition of Tn21 occurs via a simple insertion mechanism.

Full text

PDF
1351

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arthur A., Nimmo E., Hettle S., Sherratt D. Transposition and transposition immunity of transposon Tn3 derivatives having different ends. EMBO J. 1984 Aug;3(8):1723–1729. doi: 10.1002/j.1460-2075.1984.tb02038.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Avila P., de la Cruz F., Ward E., Grinsted J. Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase. Mol Gen Genet. 1984;195(1-2):288–293. doi: 10.1007/BF00332761. [DOI] [PubMed] [Google Scholar]
  3. Beck E., Ludwig G., Auerswald E. A., Reiss B., Schaller H. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene. 1982 Oct;19(3):327–336. doi: 10.1016/0378-1119(82)90023-3. [DOI] [PubMed] [Google Scholar]
  4. Clark A. J., Warren G. J. Conjugal transmission of plasmids. Annu Rev Genet. 1979;13:99–125. doi: 10.1146/annurev.ge.13.120179.000531. [DOI] [PubMed] [Google Scholar]
  5. Datta N., Hedges R. W. Trimethoprim resistance conferred by W plasmids in Enterobacteriaceae. J Gen Microbiol. 1972 Sep;72(2):349–355. doi: 10.1099/00221287-72-2-349. [DOI] [PubMed] [Google Scholar]
  6. Diaz-Aroca E., de la Cruz F., Zabala J. C., Ortiz J. M. Characterization of the new insertion sequence IS91 from an alpha-hemolysin plasmid of Escherichia coli. Mol Gen Genet. 1984;193(3):493–499. doi: 10.1007/BF00382089. [DOI] [PubMed] [Google Scholar]
  7. Galas D. J., Chandler M. On the molecular mechanisms of transposition. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4858–4862. doi: 10.1073/pnas.78.8.4858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grindley N. D., Reed R. R. Transpositional recombination in prokaryotes. Annu Rev Biochem. 1985;54:863–896. doi: 10.1146/annurev.bi.54.070185.004243. [DOI] [PubMed] [Google Scholar]
  9. Groenen M. A., Kokke M., van de Putte P. Transposition of mini-Mu containing only one of the ends of bacteriophage Mu. EMBO J. 1986 Dec 20;5(13):3687–3690. doi: 10.1002/j.1460-2075.1986.tb04700.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heritage J., Bennett P. M. Plasmid fusions mediated by one end of TnA. J Gen Microbiol. 1985 May;131(5):1130–1140. doi: 10.1099/00221287-131-5-1131. [DOI] [PubMed] [Google Scholar]
  11. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  13. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  14. Mötsch S., Schmitt R., Avila P., de la Cruz F., Ward E., Grinsted J. Junction sequences generated by 'one-ended transposition'. Nucleic Acids Res. 1985 May 10;13(9):3335–3342. doi: 10.1093/nar/13.9.3335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mötsch S., Schmitt R. Replicon fusion mediated by a single-ended derivative of transposon Tn1721. Mol Gen Genet. 1984;195(1-2):281–287. doi: 10.1007/BF00332760. [DOI] [PubMed] [Google Scholar]
  16. Novick R. P., Clowes R. C., Cohen S. N., Curtiss R., 3rd, Datta N., Falkow S. Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev. 1976 Mar;40(1):168–189. doi: 10.1128/br.40.1.168-189.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rüther U. pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts. Nucleic Acids Res. 1982 Oct 11;10(19):5765–5772. doi: 10.1093/nar/10.19.5765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. de la Cruz F., Grinsted J. Genetic and molecular characterization of Tn21, a multiple resistance transposon from R100.1. J Bacteriol. 1982 Jul;151(1):222–228. doi: 10.1128/jb.151.1.222-228.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES