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. 1988 Apr;170(4):1958–1961. doi: 10.1128/jb.170.4.1958-1961.1988

RNA polymerase-binding and transcription initiation sites upstream of the methyl reductase operon of Methanococcus vannielii.

M Thomm 1, B A Sherf 1, J N Reeve 1
PMCID: PMC211058  PMID: 2832392

Abstract

RNA polymerase, purified from Methanococcus vannielii, was shown by exonuclease III footprinting to bind to a 49-base-pair (bp) region of DNA in the intergenic region upstream of mcrB. S1 nuclease protection experiments demonstrated that transcription initiation in vivo occurs within this region at 32 or 33 bp 5' to the ATG translation initiation codon of mcrB and 19 or 20 bp 3' to a TATA box.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cram D. S., Sherf B. A., Libby R. T., Mattaliano R. J., Ramachandran K. L., Reeve J. N. Structure and expression of the genes, mcrBDCGA, which encode the subunits of component C of methyl coenzyme M reductase in Methanococcus vannielii. Proc Natl Acad Sci U S A. 1987 Jun;84(12):3992–3996. doi: 10.1073/pnas.84.12.3992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hamilton P. T., Reeve J. N. Structure of genes and an insertion element in the methane producing archaebacterium Methanobrevibacter smithii. Mol Gen Genet. 1985;200(1):47–59. doi: 10.1007/BF00383311. [DOI] [PubMed] [Google Scholar]
  3. Reiter W. D., Palm P., Voos W., Kaniecki J., Grampp B., Schulz W., Zillig W. Putative promoter elements for the ribosomal RNA genes of the thermoacidophilic archaebacterium Sulfolobus sp. strain B12. Nucleic Acids Res. 1987 Jul 24;15(14):5581–5595. doi: 10.1093/nar/15.14.5581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Shalloway D., Kleinberger T., Livingston D. M. Mapping of SV40 DNA replication origin region binding sites for the SV40 T antigen by protection against exonuclease III digestion. Cell. 1980 Jun;20(2):411–422. doi: 10.1016/0092-8674(80)90627-3. [DOI] [PubMed] [Google Scholar]
  5. Thomm M., Madon J., Stetter K. O. DNA-dependent RNA polymerases of the three orders of methanogens. Biol Chem Hoppe Seyler. 1986 Jun;367(6):473–481. doi: 10.1515/bchm3.1986.367.1.473. [DOI] [PubMed] [Google Scholar]
  6. Thomm M., Stetter K. O. Transcription in methanogens. Evidence for specific in vitro transcription of the purified DNA-dependent RNA polymerase of Methanococcus thermolithotrophicus. Eur J Biochem. 1985 Jun 3;149(2):345–351. doi: 10.1111/j.1432-1033.1985.tb08932.x. [DOI] [PubMed] [Google Scholar]
  7. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  8. Wich G., Hummel H., Jarsch M., Bär U., Böck A. Transcription signals for stable RNA genes in Methanococcus. Nucleic Acids Res. 1986 Mar 25;14(6):2459–2479. doi: 10.1093/nar/14.6.2459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wich G., Leinfelder W., Böck A. Genes for stable RNA in the extreme thermophile Thermoproteus tenax: introns and transcription signals. EMBO J. 1987 Feb;6(2):523–528. doi: 10.1002/j.1460-2075.1987.tb04784.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wu C. An exonuclease protection assay reveals heat-shock element and TATA box DNA-binding proteins in crude nuclear extracts. Nature. 1985 Sep 5;317(6032):84–87. doi: 10.1038/317084a0. [DOI] [PubMed] [Google Scholar]

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