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. 1988 Jul;170(7):3301–3304. doi: 10.1128/jb.170.7.3301-3304.1988

Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments.

P M Ahrweiler 1, C Frieden 1
PMCID: PMC211288  PMID: 3290203

Abstract

A strain of Escherichia coli with the fol gene deleted and a kan gene inserted in its place was created for use in cloning and isolation of mutant dihydrofolate reductase. Southern blot analysis and dihydrofolate reductase enzyme assays confirmed the delta fol::kan genotype. A thyA mutation accompanied the fol deletion and is required for survival of a dihydrofolate reductase-deficient strain.

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Selected References

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