Abstract
A new two-dimensional ribosome crystal, having the tetragonal space group P42(1)2 (a = 593 A), has been grown from ribosome tetramers extracted from hypothermic chick embryos. It is of particular interest because of its larger size (up to 3 x 3 micron2) and greater stability compared to other related polymorphic forms, and because it can easily be grown in large amounts. X-ray diffraction shows the order in the crystal to extend to a resolution of at least 60 A. The crystalline ribosomes appear to contain a full complement of small and large ribosomal subunit proteins and an additional four proteins not characteristic of chick embryo polysomes.
Full Text
The Full Text of this article is available as a PDF (2.1 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barbieri M. Ribosome crystallization in homogenates and cell extracts of chick embryos. J Supramol Struct. 1979;10(3):349–357. doi: 10.1002/jss.400100306. [DOI] [PubMed] [Google Scholar]
- Byers B. Ribosome crystallization induced in chick embryo tissues by hypothermia. J Cell Biol. 1966 Sep;30(3):C1–C6. [PMC free article] [PubMed] [Google Scholar]
- Byers B. Structure and formation of ribosome crystals in hypothermic chick embryo cells. J Mol Biol. 1967 Jun 14;26(2):155–167. doi: 10.1016/0022-2836(67)90288-4. [DOI] [PubMed] [Google Scholar]
- Clark M. W., Hammons M., Langer J. A., Lake J. A. Helical arrays of Escherichia coli small ribosomal subunits produced in vitro. J Mol Biol. 1979 Dec 5;135(2):507–512. doi: 10.1016/0022-2836(79)90450-9. [DOI] [PubMed] [Google Scholar]
- Clark M. W., Leonard K., Lake J. A. Ribosomal crystalline arrays of large subunits from Escherichia coli. Science. 1982 May 28;216(4549):999–1001. doi: 10.1126/science.7043735. [DOI] [PubMed] [Google Scholar]
- Hardy S. J., Kurland C. G., Voynow P., Mora G. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry. 1969 Jul;8(7):2897–2905. doi: 10.1021/bi00835a031. [DOI] [PubMed] [Google Scholar]
- LANGRIDGE R. Ribosomes: a common structural feature. Science. 1963 May 31;140(3570):1000–1000. doi: 10.1126/science.140.3570.1000. [DOI] [PubMed] [Google Scholar]
- Lastick S. M., McConkey E. H. Exchange and stability of HeLa ribosomal proteins in vivo. J Biol Chem. 1976 May 25;251(10):2867–2875. [PubMed] [Google Scholar]
- McConkey E. H., Bielka H., Gordon J., Lastick S. M., Lin A., Ogata K., Reboud J. P., Traugh J. A., Traut R. R., Warner J. R. Proposed uniform nomenclature for mammalian ribosomal proteins. Mol Gen Genet. 1979 Jan 16;169(1):1–6. doi: 10.1007/BF00267538. [DOI] [PubMed] [Google Scholar]
- Morgan R. S., Uzman B. G. Nature of the packing of ribosomes within chromatoid bodies. Science. 1966 Apr 8;152(3719):214–216. doi: 10.1126/science.152.3719.214. [DOI] [PubMed] [Google Scholar]
- Morimoto T., Blobel G., Sabatini D. D. Ribosome crystallization in chicken embryos. I. Isolation, characterization, and in vitro activity of ribosome tetramers. J Cell Biol. 1972 Feb;52(2):338–354. doi: 10.1083/jcb.52.2.338. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morimoto T., Blobel G., Sabatini D. D. Ribosome crystallization in chicken embryos. II. Conditions for the formation of ribosome tetramers in vitro. J Cell Biol. 1972 Feb;52(2):355–366. doi: 10.1083/jcb.52.2.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taddei C. Ribosome arrangement during oogenesis of Lacerta sicula Raf. Exp Cell Res. 1972 Feb;70(2):285–292. doi: 10.1016/0014-4827(72)90138-3. [DOI] [PubMed] [Google Scholar]
- Unwin P. N., Taddei C. Packing of ribosomes in crystals from the lizard Lacerta sicula. J Mol Biol. 1977 Aug 25;114(4):491–506. doi: 10.1016/0022-2836(77)90174-7. [DOI] [PubMed] [Google Scholar]
- Warner J. R., Gorenstein C. The ribosomal proteins of Saccharomyces cerevisiae. Methods Cell Biol. 1978;20:45–60. doi: 10.1016/s0091-679x(08)62008-7. [DOI] [PubMed] [Google Scholar]