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. 1988 Sep;170(9):3978–3982. doi: 10.1128/jb.170.9.3978-3982.1988

Efficiency of homologous DNA recombination varies along the Bacillus subtilis chromosome.

V Vagner 1, S D Ehrlich 1
PMCID: PMC211398  PMID: 3137211

Abstract

Structures consisting of a genetic marker (erythromycin or kanamycin resistance, thymidylate synthetase) flanked by 3.4-kilobase direct repeats (pBR322 sequences) were inserted in 12 different locations of the Bacillus subtilis chromosome. Recombination between the repeats was followed by the loss of the genetic marker. Recombination frequencies found in different locations varied from 1.2 X 10(-5) to 40 X 10(-5) per cell generation. Such differences were highly significant (P less than 0.001).

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Selected References

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