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. 1988 Sep;170(9):4411–4414. doi: 10.1128/jb.170.9.4411-4414.1988

Purification of Rts1 RepA protein and binding of the protein to mini-Rts1 DNA.

Y Kamio 1, Y Itoh 1, Y Terawaki 1
PMCID: PMC211463  PMID: 3045097

Abstract

RepA protein, essential for the replication of plasmid Rts1, was purified, and its binding to mini-Rts1 subregions was examined by a DNase I protection assay. RepA protected the incI and incII iterons, a region immediately upstream of the repA promoter, and a 10-base-pair region located between the most external incII iteron and a GATC box. The protection was less efficient when preheated RepA was used.

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Selected References

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