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. 1987 Jul 1;105(1):247–250. doi: 10.1083/jcb.105.1.247

Import of the carboxy-terminal portion of acyl-CoA oxidase into peroxisomes of Candida tropicalis

PMCID: PMC2114900  PMID: 3611187

Abstract

We report the sequence of a cDNA clone that codes for the carboxy- terminal portion of the peroxisomal protein, acyl-CoA oxidase, from the yeast, Candida tropicalis. This is a newly identified acyl-CoA oxidase sequence, most likely a second allele of POX4. The cDNA clone was expressed by in vitro transcription followed by translation. The major product, a 43-kD protein, associated with isolated peroxisomes in an in vitro import assay. More than half of the peroxisome-associated protein was protected from added protease, implying that it was internalized within the organelle. These findings indicate that there is sufficient information in the carboxy-terminal portion of the protein to target it to peroxisomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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