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. 1988 Oct;170(10):4942–4945. doi: 10.1128/jb.170.10.4942-4945.1988

Integration and mapping of Bacillus megaterium genes which code for small, acid-soluble spore proteins and their protease.

M D Sussman 1, P S Vary 1, C Hartman 1, P Setlow 1
PMCID: PMC211542  PMID: 3139641

Abstract

Four genes (ssp genes) coding for small, acid-soluble spore proteins of Bacillus megaterium and the gene for the protease that cleaves them during germination were cloned in the integratable plasmid pJH101. Each plasmid was integrated into the B. megaterium chromosome by a Campbell-type mechanism, allowing mapping of all five genes. The gene for the small, acid-soluble spore protein-specific protease (gpr) mapped near rib, and the sspA gene mapped between argA and hisA. The three other genes of the spp gene family (sspB, -D, and -F) all mapped near metC/D, with the order: sspF-sspD-metC/D-hemA-argO-sspB. While neither gpr nor sspF has been mapped in B. subtilis, the positions of the sspA, -B, and -D loci are similar in B. megaterium and B. subtilis, suggesting that the members of this multigene family have not recently undergone significant movement on the chromosome. It appears that more gene rearrangement has occurred in the flanking genes than has occurred in the ssp family of genes producing the small, acid-soluble spore proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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