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. 1988 Dec;170(12):5785–5788. doi: 10.1128/jb.170.12.5785-5788.1988

Complementation and hybridization evidence for additional families of type I DNA restriction and modification genes in Salmonella serotypes.

J Ryu 1, P T Rajadas 1, L R Bullas 1
PMCID: PMC211683  PMID: 3056913

Abstract

Of eight Salmonella, serB-linked hsd genes for the restriction and modification of DNA transferred to Escherichia coli/Salmonella hybrids, only two--those with SM and ST (S. muenchen and S. thompson, respectively) specificities--may have weakly complemented rSB- and none complemented rK-. An A-specific DNA probe failed to hybridize to HindIII-restricted fragments of each of the hybrids, but an SB (S. typhimurium)-specific probe hybridized to DNA from the hybrid with ST specificity. These results indicate that additional families of the type I hsd genes may exist.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYER H. GENETIC CONTROL OF RESTRICTION AND MODIFICATION IN ESCHERICHIA COLI. J Bacteriol. 1964 Dec;88:1652–1660. doi: 10.1128/jb.88.6.1652-1660.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyer H. W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969 May 14;41(3):459–472. doi: 10.1016/0022-2836(69)90288-5. [DOI] [PubMed] [Google Scholar]
  3. Bullas L. R., Colson C. DNA restriction and modification systems in Salmonella. III. SP, a Salmonella potsdam system allelic to the SB system in Salmonella typhimurium. Mol Gen Genet. 1975 Aug 27;139(3):177–188. [PubMed] [Google Scholar]
  4. Bullas L. R., Colson C., Neufeld B. Deoxyribonucleic acid restriction and modification systems in Salmonella: chromosomally located systems of different serotypes. J Bacteriol. 1980 Jan;141(1):275–292. doi: 10.1128/jb.141.1.275-292.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bullas L. R., Colson C., Van Pel A. DNA restriction and modification systems in Salmonella. SQ, a new system derived by recombination between the SB system of Salmonella typhimurium and the SP system of Salmonella potsdam. J Gen Microbiol. 1976 Jul;95(1):166–172. doi: 10.1099/00221287-95-1-166. [DOI] [PubMed] [Google Scholar]
  6. Colson C., Van Pel A. DNA restriction and modification systems in Salmonella. I. SA and SB, two Salmonella typhimurium systems determined by genes with a chromosomal location comparable to that of the Escherichia coli hsd genes. Mol Gen Genet. 1974 Apr 3;129(4):325–337. doi: 10.1007/BF00265696. [DOI] [PubMed] [Google Scholar]
  7. Daniel A. S., Fuller-Pace F. V., Legge D. M., Murray N. E. Distribution and diversity of hsd genes in Escherichia coli and other enteric bacteria. J Bacteriol. 1988 Apr;170(4):1775–1782. doi: 10.1128/jb.170.4.1775-1782.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Firman K., Price C., Glover S. W. The EcoR124 and EcoR124/3 restriction and modification systems: cloning the genes. Plasmid. 1985 Nov;14(3):224–234. doi: 10.1016/0147-619x(85)90006-x. [DOI] [PubMed] [Google Scholar]
  9. Fuller-Pace F. V., Bullas L. R., Delius H., Murray N. E. Genetic recombination can generate altered restriction specificity. Proc Natl Acad Sci U S A. 1984 Oct;81(19):6095–6099. doi: 10.1073/pnas.81.19.6095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fuller-Pace F. V., Cowan G. M., Murray N. E. EcoA and EcoE: alternatives to the EcoK family of type I restriction and modification systems of Escherichia coli. J Mol Biol. 1985 Nov 5;186(1):65–75. doi: 10.1016/0022-2836(85)90257-8. [DOI] [PubMed] [Google Scholar]
  11. Fuller-Pace F. V., Murray N. E. Two DNA recognition domains of the specificity polypeptides of a family of type I restriction enzymes. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9368–9372. doi: 10.1073/pnas.83.24.9368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Glover S. W., Colson C. Genetics of host-controlled restriction and modification in Escherichia coli. Genet Res. 1969 Apr;13(2):227–240. doi: 10.1017/s0016672300002901. [DOI] [PubMed] [Google Scholar]
  13. Glover S. W. Functional analysis of host-specificity mutants in Escherichia coli. Genet Res. 1970 Apr;15(2):237–250. doi: 10.1017/s0016672300001567. [DOI] [PubMed] [Google Scholar]
  14. Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Murray N. E., Gough J. A., Suri B., Bickle T. A. Structural homologies among type I restriction-modification systems. EMBO J. 1982;1(5):535–539. doi: 10.1002/j.1460-2075.1982.tb01205.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Piekarowicz A., Goguen J. D., Skrzypek E. The EcoDXX1 restriction and modification system of Escherichia coli ET7. Purification, subunit structure and properties of the restriction endonuclease. Eur J Biochem. 1985 Oct 15;152(2):387–393. doi: 10.1111/j.1432-1033.1985.tb09209.x. [DOI] [PubMed] [Google Scholar]
  18. Sain B., Murray N. E. The hsd (host specificity) genes of E. coli K 12. Mol Gen Genet. 1980;180(1):35–46. doi: 10.1007/BF00267350. [DOI] [PubMed] [Google Scholar]
  19. Suri B., Bickle T. A. EcoA: the first member of a new family of type I restriction modification systems. Gene organization and enzymatic activities. J Mol Biol. 1985 Nov 5;186(1):77–85. doi: 10.1016/0022-2836(85)90258-x. [DOI] [PubMed] [Google Scholar]
  20. Van Pel A., Colson C. DNA restriction and modification systems in Salmonella. II. Genetic complementation between the K and B systems of Escherichia coli and the Salmonella typhimurium system SB, with the same chromosomal location. Mol Gen Genet. 1974;135(1):51–60. doi: 10.1007/BF00433901. [DOI] [PubMed] [Google Scholar]
  21. Yuan R., Hamilton D. L. Restriction and modification of DNA by a complex protein. Am Sci. 1982 Jan-Feb;70(1):61–69. [PubMed] [Google Scholar]

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