Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Dec;170(12):5806–5813. doi: 10.1128/jb.170.12.5806-5813.1988

Analysis of recombination occurring at SLP1 att sites.

S C Lee 1, C A Omer 1, M A Brasch 1, S N Cohen 1
PMCID: PMC211686  PMID: 3056916

Abstract

SLP1int is a conjugative Streptomyces coelicolor genetic element that can transfer to Streptomyces lividans and integrate site specifically into the genome of the new bacterial host. Recombination of SLP1 previously has been shown to occur within nearly identical 112-base-pair att sequences on the plasmid and host chromosome. We report here that both integrative recombination and intermolecular transfer of SLP1int require no more than a 48-base-pair segment of the att sequence and that SLP1 transfer occurs by a conservative rather than a replicative mechanism. The functions responsible for the excision of the element as a discrete DNA segment are induced during the conjugal transfer of SLP1.

Full text

PDF
5807

Images in this article

5811Image
on p.5811

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bibb M. J., Ward J. M., Kieser T., Cohen S. N., Hopwood D. A. Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividans. Mol Gen Genet. 1981;184(2):230–240. doi: 10.1007/BF00272910. [DOI] [PubMed] [Google Scholar]
  2. Broach J. R., Guarascio V. R., Jayaram M. Recombination within the yeast plasmid 2mu circle is site-specific. Cell. 1982 May;29(1):227–234. doi: 10.1016/0092-8674(82)90107-6. [DOI] [PubMed] [Google Scholar]
  3. Casadaban M. J., Cohen S. N. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179–207. doi: 10.1016/0022-2836(80)90283-1. [DOI] [PubMed] [Google Scholar]
  4. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohen A., Bar-Nir D., Goedeke M. E., Parag Y. The integrated and free states of Streptomyces griseus plasmid pSG1. Plasmid. 1985 Jan;13(1):41–50. doi: 10.1016/0147-619x(85)90054-x. [DOI] [PubMed] [Google Scholar]
  6. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  8. Grindley N. D., Reed R. R. Transpositional recombination in prokaryotes. Annu Rev Biochem. 1985;54:863–896. doi: 10.1146/annurev.bi.54.070185.004243. [DOI] [PubMed] [Google Scholar]
  9. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hartley J. L., Donelson J. E. Nucleotide sequence of the yeast plasmid. Nature. 1980 Aug 28;286(5776):860–865. doi: 10.1038/286860a0. [DOI] [PubMed] [Google Scholar]
  11. Hoess R. H., Ziese M., Sternberg N. P1 site-specific recombination: nucleotide sequence of the recombining sites. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3398–3402. doi: 10.1073/pnas.79.11.3398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hopwood D. A., Hintermann G., Kieser T., Wright H. M. Integrated DNA sequences in three streptomycetes form related autonomous plasmids after transfer to Streptomyces lividans. Plasmid. 1984 Jan;11(1):1–16. doi: 10.1016/0147-619x(84)90002-7. [DOI] [PubMed] [Google Scholar]
  13. Hopwood D. A., Kieser T., Wright H. M., Bibb M. J. Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. J Gen Microbiol. 1983 Jul;129(7):2257–2269. doi: 10.1099/00221287-129-7-2257. [DOI] [PubMed] [Google Scholar]
  14. Jayaram M. Two-micrometer circle site-specific recombination: the minimal substrate and the possible role of flanking sequences. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5875–5879. doi: 10.1073/pnas.82.17.5875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Katz E., Thompson C. J., Hopwood D. A. Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans. J Gen Microbiol. 1983 Sep;129(9):2703–2714. doi: 10.1099/00221287-129-9-2703. [DOI] [PubMed] [Google Scholar]
  16. Kendall K., Cullum J. Cloning and expression of an extracellular-agarase from Streptomyces coelicolor A3(2) in Streptomyces lividans 66. Gene. 1984 Sep;29(3):315–321. doi: 10.1016/0378-1119(84)90060-x. [DOI] [PubMed] [Google Scholar]
  17. Kieser T. Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid. 1984 Jul;12(1):19–36. doi: 10.1016/0147-619x(84)90063-5. [DOI] [PubMed] [Google Scholar]
  18. Madon J., Moretti P., Hütter R. Site-specific integration and excision of pMEA100 in Nocardia mediterranei. Mol Gen Genet. 1987 Sep;209(2):257–264. doi: 10.1007/BF00329651. [DOI] [PubMed] [Google Scholar]
  19. Moretti P., Hintermann G., Hütter R. Isolation and characterization of an extrachromosomal element from Nocardia mediterranei. Plasmid. 1985 Sep;14(2):126–133. doi: 10.1016/0147-619x(85)90072-1. [DOI] [PubMed] [Google Scholar]
  20. Omer C. A., Cohen S. N. Plasmid formation in Streptomyces: excision and integration of the SLP1 replicon at a specific chromosomal site. Mol Gen Genet. 1984;196(3):429–438. doi: 10.1007/BF00436190. [DOI] [PubMed] [Google Scholar]
  21. Omer C. A., Cohen S. N. Structural analysis of plasmid and chromosomal loci involved in site-specific excision and integration of the SLP1 element of Streptomyces coelicolor. J Bacteriol. 1986 Jun;166(3):999–1006. doi: 10.1128/jb.166.3.999-1006.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Omer C. A., Stein D., Cohen S. N. Site-specific insertion of biologically functional adventitious genes into the Streptomyces lividans chromosome. J Bacteriol. 1988 May;170(5):2174–2184. doi: 10.1128/jb.170.5.2174-2184.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pernodet J. L., Simonet J. M., Guérineau M. Plasmids in different strains of Streptomyces ambofaciens: free and integrated form of plasmid pSAM2. Mol Gen Genet. 1984;198(2):35–41. doi: 10.1007/BF00328697. [DOI] [PubMed] [Google Scholar]
  24. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  25. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  27. Tsai J. F., Chen C. W. Isolation and characterization of Streptomyces lividans mutants deficient in intraplasmid recombination. Mol Gen Genet. 1987 Jun;208(1-2):211–218. doi: 10.1007/BF00330444. [DOI] [PubMed] [Google Scholar]
  28. WOLLMAN E. L., JACOB F. Lysogénie et recombinaison génétique chez Escherichia coli K12. C R Hebd Seances Acad Sci. 1954 Aug 2;239(5):455–456. [PubMed] [Google Scholar]
  29. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES