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. 1988 Dec;170(12):5870–5876. doi: 10.1128/jb.170.12.5870-5876.1988

Genetic manipulation of major P-fimbrial subunits and consequences for formation of fimbriae.

I Van Die 1, M Wauben 1, I Van Megen 1, H Bergmans 1, N Riegman 1, W Hoekstra 1, P Pouwels 1, B Enger-Valk 1
PMCID: PMC211694  PMID: 2903858

Abstract

The influence of genetic manipulation of the structural genes coding for major P-fimbrial subunits on the formation of fimbriae in Escherichia coli was studied. Deletion of two regions that code for hypervariable parts of the P fimbrillin resulted in strong reduction or total absence of fimbria production. Replacement of deleted amino acids by other amino acid residues restored the formation of fimbriae. The hypervariable regions may be important for biogenesis of fimbriae by imposing correct spacing between conserved regions of the protein. The potential for substituting amino acids in the P-fimbrial subunit opens interesting possibilities for use of fimbriae as carriers of foreign antigenic determinants. An antigenic determinant of foot-and-mouth disease virus (FMDV) was incorporated in the F11 fimbrial subunit. Hybrid fimbriae, recognized by an FMDV-specific neutralizing monoclonal antibody directed against FMDV, were formed.

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Selected References

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