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. 1988 Dec;170(12):5925–5927. doi: 10.1128/jb.170.12.5925-5927.1988

High-frequency transformation of Rhizobium meliloti.

J Courtois 1, B Courtois 1, J Guillaume 1
PMCID: PMC211705  PMID: 3056925

Abstract

Transformation of R factor RP4 and its derivative pRK290 from Escherichia coli to Rhizobium meliloti is reported. The efficiency of transformation was in the range of 10(-5) per viable cell. In addition, chromosomal DNA prepared from one R. meliloti strain resistant to streptomycin was transferred to the isoleucine-valine-requiring mutant susceptible to streptomycin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balassa G. GENETIC TRANSFORMATION OF RHIZOBIUM: A REVIEW OF THE WORK OF R. BALASSA. Bacteriol Rev. 1963 Jun;27(2):228–241. doi: 10.1128/br.27.2.228-241.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beringer J. E. R factor transfer in Rhizobium leguminosarum. J Gen Microbiol. 1974 Sep;84(1):188–198. doi: 10.1099/00221287-84-1-188. [DOI] [PubMed] [Google Scholar]
  3. Courtois B., Hornez J. P., Courtois J., Derieux J. C. Mise en évidence d'une properiété métabolique de Rhizobium meliloti utilisable pour sa classification. Ann Microbiol (Paris) 1983 Mar-Apr;134A(2):141–147. [PubMed] [Google Scholar]
  4. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. Properties of an R factor from Pseudomonas aeruginosa. J Bacteriol. 1971 Dec;108(3):1244–1249. doi: 10.1128/jb.108.3.1244-1249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ditta G., Stanfield S., Corbin D., Helinski D. R. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347–7351. doi: 10.1073/pnas.77.12.7347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Drozańska D., Lorkiewicz Z. Genetic transformation in Rhizobium trifolii. Acta Microbiol Pol. 1978;27(2):81–88. [PubMed] [Google Scholar]
  7. Eckhardt T. A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. Plasmid. 1978 Sep;1(4):584–588. doi: 10.1016/0147-619x(78)90016-1. [DOI] [PubMed] [Google Scholar]
  8. Jouanin L., De Lajudie P., Bazetoux S., Huguet T. DNA sequence homology in Rhizobium meliloti plasmids. Mol Gen Genet. 1981;182(2):189–195. doi: 10.1007/BF00269657. [DOI] [PubMed] [Google Scholar]
  9. Kiss G. B., Kálmán Z. Transformation of Rhizobium meliloti 41 with plasmid DNA. J Bacteriol. 1982 May;150(2):465–470. doi: 10.1128/jb.150.2.465-470.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LURIA S. E., BURROUS J. W. Hybridization between Escherichia coli and Shigella. J Bacteriol. 1957 Oct;74(4):461–476. doi: 10.1128/jb.74.4.461-476.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'Gara F., Dunican L. K. Transformation and physical properties of R-factor RP4 transferred from Escherichia coli to Rhizobium trifolii. J Bacteriol. 1973 Dec;116(3):1177–1180. doi: 10.1128/jb.116.3.1177-1180.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Raina J. L., Modi V. V. Further studies on genetic transformation in Rhizobium. J Gen Microbiol. 1971 Feb;65(2):161–165. doi: 10.1099/00221287-65-2-161. [DOI] [PubMed] [Google Scholar]
  13. Selvaraj G., Iyer V. N. Genetic transformation of Rhizobium meliloti by plasmid DNA. Gene. 1981 Nov;15(2-3):279–283. doi: 10.1016/0378-1119(81)90137-2. [DOI] [PubMed] [Google Scholar]

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