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. 1987 Feb;169(2):880–884. doi: 10.1128/jb.169.2.880-884.1987

A Rhizobium meliloti mutant that forms ineffective pseudonodules in alfalfa produces exopolysaccharide but fails to form beta-(1----2) glucan.

R A Geremia, S Cavaignac, A Zorreguieta, N Toro, J Olivares, R A Ugalde
PMCID: PMC211861  PMID: 3804979

Abstract

A mutant of Rhizobium meliloti that elicited the formation of inactive nodules in alfalfa was found not to form beta-(1----2) glucan in vivo or in vitro. It was nonmotile because it lacks flagella. The 235-kilodalton protein which acts as an intermediate in beta-(1----2) glucan synthesis was undetectable in the mutant. These properties of the mutant are common to those of chvB mutants of Agrobacterium tumefaciens. Exopolysaccharide formation by the R. meliloti mutant was about double that by the wild type.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe M., Sherwood J. E., Hollingsworth R. I., Dazzo F. B. Stimulation of clover root hair infection by lectin-binding oligosaccharides from the capsular and extracellular polysaccharides of Rhizobium trifolii. J Bacteriol. 1984 Nov;160(2):517–520. doi: 10.1128/jb.160.2.517-520.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bánfalvi Z., Sakanyan V., Koncz C., Kiss A., Dusha I., Kondorosi A. Location of nodulation and nitrogen fixation genes on a high molecular weight plasmid of R. meliloti. Mol Gen Genet. 1981;184(2):318–325. doi: 10.1007/BF00272925. [DOI] [PubMed] [Google Scholar]
  3. DUBOIS M., GILLES K., HAMILTON J. K., REBERS P. A., SMITH F. A colorimetric method for the determination of sugars. Nature. 1951 Jul 28;168(4265):167–167. doi: 10.1038/168167a0. [DOI] [PubMed] [Google Scholar]
  4. Douglas C. J., Staneloni R. J., Rubin R. A., Nester E. W. Identification and genetic analysis of an Agrobacterium tumefaciens chromosomal virulence region. J Bacteriol. 1985 Mar;161(3):850–860. doi: 10.1128/jb.161.3.850-860.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dylan T., Ielpi L., Stanfield S., Kashyap L., Douglas C., Yanofsky M., Nester E., Helinski D. R., Ditta G. Rhizobium meliloti genes required for nodule development are related to chromosomal virulence genes in Agrobacterium tumefaciens. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4403–4407. doi: 10.1073/pnas.83.12.4403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Finan T. M., Hirsch A. M., Leigh J. A., Johansen E., Kuldau G. A., Deegan S., Walker G. C., Signer E. R. Symbiotic mutants of Rhizobium meliloti that uncouple plant from bacterial differentiation. Cell. 1985 Apr;40(4):869–877. doi: 10.1016/0092-8674(85)90346-0. [DOI] [PubMed] [Google Scholar]
  7. Fisher R. F., Tu J. K., Long S. R. Conserved Nodulation Genes in Rhizobium meliloti and Rhizobium trifolii. Appl Environ Microbiol. 1985 Jun;49(6):1432–1435. doi: 10.1128/aem.49.6.1432-1435.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Handelsman J., Ugalde R. A., Brill W. J. Rhizobium meliloti competitiveness and the alfalfa agglutinin. J Bacteriol. 1984 Mar;157(3):703–707. doi: 10.1128/jb.157.3.703-707.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jacobs T. W., Egelhoff T. T., Long S. R. Physical and genetic map of a Rhizobium meliloti nodulation gene region and nucleotide sequence of nodC. J Bacteriol. 1985 May;162(2):469–476. doi: 10.1128/jb.162.2.469-476.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leigh J. A., Signer E. R., Walker G. C. Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6231–6235. doi: 10.1073/pnas.82.18.6231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Miller K. J., Kennedy E. P., Reinhold V. N. Osmotic adaptation by gram-negative bacteria: possible role for periplasmic oligosaccharides. Science. 1986 Jan 3;231(4733):48–51. doi: 10.1126/science.3941890. [DOI] [PubMed] [Google Scholar]
  12. Puvanesarajah V., Schell F. M., Stacey G., Douglas C. J., Nester E. W. Role for 2-linked-beta-D-glucan in the virulence of Agrobacterium tumefaciens. J Bacteriol. 1985 Oct;164(1):102–106. doi: 10.1128/jb.164.1.102-106.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rosenberg C., Boistard P., Dénarié J., Casse-Delbart F. Genes controlling early and late functions in symbiosis are located on a megaplasmid in Rhizobium meliloti. Mol Gen Genet. 1981;184(2):326–333. doi: 10.1007/BF00272926. [DOI] [PubMed] [Google Scholar]
  14. SLONEKER J. H., ORENTAS D. G. Pyruvic acid, a unique component of an exocellular bacterial polysaccharide. Nature. 1962 May 5;194:478–479. doi: 10.1038/194478a0. [DOI] [PubMed] [Google Scholar]
  15. Toro N., Olivares J. Analysis of Rhizobium meliloti Sym Mutants Obtained by Heat Treatment. Appl Environ Microbiol. 1986 May;51(5):1148–1150. doi: 10.1128/aem.51.5.1148-1150.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ugalde R. A., Coira J. A., Brill W. J. Biosynthesis of a galactose-and galacturonic acid-containing polysaccharide in Rhizobium meliloti. J Bacteriol. 1986 Oct;168(1):270–275. doi: 10.1128/jb.168.1.270-275.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ugalde R. A., Handelsman J., Brill W. J. Role of galactosyltransferase activity in phage sensitivity and nodulation competitiveness of Rhizobium meliloti. J Bacteriol. 1986 Apr;166(1):148–154. doi: 10.1128/jb.166.1.148-154.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Zorreguieta A., Tolmasky M. E., Staneloni R. J. The enzymatic synthesis of beta 1-2 glucans. Arch Biochem Biophys. 1985 May 1;238(2):368–372. doi: 10.1016/0003-9861(85)90176-6. [DOI] [PubMed] [Google Scholar]
  19. Zorreguieta A., Ugalde R. A. Formation in Rhizobium and Agrobacterium spp. of a 235-kilodalton protein intermediate in beta-D(1-2) glucan synthesis. J Bacteriol. 1986 Sep;167(3):947–951. doi: 10.1128/jb.167.3.947-951.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zorreguieta A., Ugalde R. A., Leloir L. F. An intermediate in cyclic beta 1-2 glucan biosynthesis. Biochem Biophys Res Commun. 1985 Jan 16;126(1):352–357. doi: 10.1016/0006-291x(85)90613-8. [DOI] [PubMed] [Google Scholar]

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