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. 1987 Mar;169(3):1182–1191. doi: 10.1128/jb.169.3.1182-1191.1987

Regulation of spo0H, an early sporulation gene in bacilli.

E J Dubnau, K Cabane, I Smith
PMCID: PMC211917  PMID: 3102457

Abstract

The construction of lacZ fusions in frame with the spo0H gene of Bacillus licheniformis enabled us to study the expression of this gene under various growth conditions and in various genetic backgrounds. spo0H was expressed during vegetative growth, but the levels increased during early stationary phase and then decreased several hours later. Expression of the gene was not repressed by glucose, but was induced by decoyinine, an inhibitor of guanine nucleotide biosynthesis, which can induce sporulation. Of those tested, the only spo0 gene required for the expression of spo0H was spo0A, and this requirement was eliminated by the abrB mutation, a partial suppressor of spo0A function. spo0H-lacZ expression was much higher in a strain with a deletion in the spo0H gene.

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Selected References

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  1. Bedwell D. M., Nomura M. Feedback regulation of RNA polymerase subunit synthesis after the conditional overproduction of RNA polymerase in Escherichia coli. Mol Gen Genet. 1986 Jul;204(1):17–23. doi: 10.1007/BF00330181. [DOI] [PubMed] [Google Scholar]
  2. Binnie C., Lampe M., Losick R. Gene encoding the sigma 37 species of RNA polymerase sigma factor from Bacillus subtilis. Proc Natl Acad Sci U S A. 1986 Aug;83(16):5943–5947. doi: 10.1073/pnas.83.16.5943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bouvier J., Stragier P., Bonamy C., Szulmajster J. Nucleotide sequence of the spo0B gene of Bacillus subtilis and regulation of its expression. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7012–7016. doi: 10.1073/pnas.81.22.7012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carter H. L., 3rd, Moran C. P., Jr New RNA polymerase sigma factor under spo0 control in Bacillus subtilis. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9438–9442. doi: 10.1073/pnas.83.24.9438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doi R. H., Wang L. F. Multiple procaryotic ribonucleic acid polymerase sigma factors. Microbiol Rev. 1986 Sep;50(3):227–243. doi: 10.1128/mr.50.3.227-243.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dubnau E., Ramakrishna N., Cabane K., Smith I. Cloning of an early sporulation gene in Bacillus subtilis. J Bacteriol. 1981 Aug;147(2):622–632. doi: 10.1128/jb.147.2.622-632.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ferrari E., Howard S. M., Hoch J. A. Effect of stage 0 sporulation mutations on subtilisin expression. J Bacteriol. 1986 Apr;166(1):173–179. doi: 10.1128/jb.166.1.173-179.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ferrari F. A., Trach K., Hoch J. A. Sequence analysis of the spo0B locus reveals a polycistronic transcription unit. J Bacteriol. 1985 Feb;161(2):556–562. doi: 10.1128/jb.161.2.556-562.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ferrari F. A., Trach K., LeCoq D., Spence J., Ferrari E., Hoch J. A. Characterization of the spo0A locus and its deduced product. Proc Natl Acad Sci U S A. 1985 May;82(9):2647–2651. doi: 10.1073/pnas.82.9.2647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fort P., Piggot P. J. Nucleotide sequence of sporulation locus spoIIA in Bacillus subtilis. J Gen Microbiol. 1984 Aug;130(8):2147–2153. doi: 10.1099/00221287-130-8-2147. [DOI] [PubMed] [Google Scholar]
  11. Freese E. B., Vasantha N., Freese E. Induction of sporulation in developmental mutants of Bacillus subtilis. Mol Gen Genet. 1979 Feb 16;170(1):67–74. doi: 10.1007/BF00268581. [DOI] [PubMed] [Google Scholar]
  12. Gilman M. Z., Chamberlin M. J. Developmental and genetic regulation of Bacillus subtilis genes transcribed by sigma 28-RNA polymerase. Cell. 1983 Nov;35(1):285–293. doi: 10.1016/0092-8674(83)90231-3. [DOI] [PubMed] [Google Scholar]
  13. Grossman A. D., Erickson J. W., Gross C. A. The htpR gene product of E. coli is a sigma factor for heat-shock promoters. Cell. 1984 Sep;38(2):383–390. doi: 10.1016/0092-8674(84)90493-8. [DOI] [PubMed] [Google Scholar]
  14. Gryczan T. J., Contente S., Dubnau D. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J Bacteriol. 1978 Apr;134(1):318–329. doi: 10.1128/jb.134.1.318-329.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gryczan T. J., Hahn J., Contente S., Dubnau D. Replication and incompatibility properties of plasmid pE194 in Bacillus subtilis. J Bacteriol. 1982 Nov;152(2):722–735. doi: 10.1128/jb.152.2.722-735.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Haldenwang W. G., Losick R. A modified RNA polymerase transcribes a cloned gene under sporulation control in Bacillus subtilis. Nature. 1979 Nov 15;282(5736):256–260. doi: 10.1038/282256a0. [DOI] [PubMed] [Google Scholar]
  17. Hoch J. A. Genetics of bacterial sporulation. Adv Genet. 1976;18:69–98. doi: 10.1016/s0065-2660(08)60437-x. [DOI] [PubMed] [Google Scholar]
  18. Hoch J. A., Trach K., Kawamura F., Saito H. Identification of the transcriptional suppressor sof-1 as an alteration in the spo0A protein. J Bacteriol. 1985 Feb;161(2):552–555. doi: 10.1128/jb.161.2.552-555.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kawamura F., Saito H. Isolation and mapping of a new suppressor mutation of an early sporulation gene spoOF mutation in Bacillus subtilis. Mol Gen Genet. 1983;192(3):330–334. doi: 10.1007/BF00392171. [DOI] [PubMed] [Google Scholar]
  20. Landick R., Vaughn V., Lau E. T., VanBogelen R. A., Erickson J. W., Neidhardt F. C. Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factor. Cell. 1984 Aug;38(1):175–182. doi: 10.1016/0092-8674(84)90538-5. [DOI] [PubMed] [Google Scholar]
  21. Lewandoski M., Dubnau E., Smith I. Transcriptional regulation of the spo0F gene of Bacillus subtilis. J Bacteriol. 1986 Nov;168(2):870–877. doi: 10.1128/jb.168.2.870-877.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Linn T., Losick R. The program of protein synthesis during sporulation in Bacillus subtilis. Cell. 1976 May;8(1):103–114. doi: 10.1016/0092-8674(76)90191-4. [DOI] [PubMed] [Google Scholar]
  23. Losick R., Pero J. Cascades of Sigma factors. Cell. 1981 Sep;25(3):582–584. doi: 10.1016/0092-8674(81)90164-1. [DOI] [PubMed] [Google Scholar]
  24. Mason J. M., Setlow P. Expression of Bacillus megaterium and Bacillus subtilis small acid-soluble spore protein genes during stationary-phase growth of asporogenous B. subtilis mutants. J Bacteriol. 1984 Mar;157(3):931–933. doi: 10.1128/jb.157.3.931-933.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Mitani T., Heinze J. E., Freese E. Induction of sporulation in Bacillus subtilis by decoyinine or hadacidin. Biochem Biophys Res Commun. 1977 Aug 8;77(3):1118–1125. doi: 10.1016/s0006-291x(77)80094-6. [DOI] [PubMed] [Google Scholar]
  26. Neidhardt F. C., VanBogelen R. A., Vaughn V. The genetics and regulation of heat-shock proteins. Annu Rev Genet. 1984;18:295–329. doi: 10.1146/annurev.ge.18.120184.001455. [DOI] [PubMed] [Google Scholar]
  27. Ollington J. F., Haldenwang W. G., Huynh T. V., Losick R. Developmentally regulated transcription in a cloned segment of the Bacillus subtilis chromosome. J Bacteriol. 1981 Aug;147(2):432–442. doi: 10.1128/jb.147.2.432-442.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Ramakrishna N., Dubnau E., Smith I. The complete DNA sequence and regulatory regions of the Bacillus licheniformis spoOH gene. Nucleic Acids Res. 1984 Feb 24;12(4):1779–1790. doi: 10.1093/nar/12.4.1779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Rong S., Rosenkrantz M. S., Sonenshein A. L. Transcriptional control of the Bacillus subtilis spoIID gene. J Bacteriol. 1986 Mar;165(3):771–779. doi: 10.1128/jb.165.3.771-779.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Rosenbluh A., Banner C. D., Losick R., Fitz-James P. C. Identification of a new developmental locus in Bacillus subtilis by construction of a deletion mutation in a cloned gene under sporulation control. J Bacteriol. 1981 Oct;148(1):341–351. doi: 10.1128/jb.148.1.341-351.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Schaeffer P., Millet J., Aubert J. P. Catabolic repression of bacterial sporulation. Proc Natl Acad Sci U S A. 1965 Sep;54(3):704–711. doi: 10.1073/pnas.54.3.704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Scheer-Abramowitz J., Gryczan T. J., Dubnau D. Origin and mode of replication of plasmids pE194 and pUB110. Plasmid. 1981 Jul;6(1):67–77. doi: 10.1016/0147-619x(81)90054-8. [DOI] [PubMed] [Google Scholar]
  33. Tatchell K. RAS genes and growth control in Saccharomyces cerevisiae. J Bacteriol. 1986 May;166(2):364–367. doi: 10.1128/jb.166.2.364-367.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Trach K. A., Chapman J. W., Piggot P. J., Hoch J. A. Deduced product of the stage 0 sporulation gene spo0F shares homology with the Spo0A, OmpR, and SfrA proteins. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7260–7264. doi: 10.1073/pnas.82.21.7260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Trempy J. E., Bonamy C., Szulmajster J., Haldenwang W. G. Bacillus subtilis sigma factor sigma 29 is the product of the sporulation-essential gene spoIIG. Proc Natl Acad Sci U S A. 1985 Jun;82(12):4189–4192. doi: 10.1073/pnas.82.12.4189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Trempy J. E., Morrison-Plummer J., Haldenwang W. G. Synthesis of sigma 29, an RNA polymerase specificity determinant, is a developmentally regulated event in Bacillus subtilis. J Bacteriol. 1985 Jan;161(1):340–346. doi: 10.1128/jb.161.1.340-346.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Trowsdale J., Chen S. M., Hoch J. A. Genetic analysis of a class of polymyxin resistant partial revertants of stage O sporulation mutants of Bacillus subtilis: map of the chromosome region near the origin of replication. Mol Gen Genet. 1979 May 23;173(1):61–70. doi: 10.1007/BF00267691. [DOI] [PubMed] [Google Scholar]
  38. Weir J., Dubnau E., Ramakrishna N., Smith I. Bacillus subtilis spo0H gene. J Bacteriol. 1984 Feb;157(2):405–412. doi: 10.1128/jb.157.2.405-412.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Yamashita S., Yoshikawa H., Kawamura F., Takahashi H., Yamamoto T., Kobayashi Y., Saito H. The effect of spo0 mutations on the expression of spo0A- and spo0F-lacZ fusions. Mol Gen Genet. 1986 Oct;205(1):28–33. doi: 10.1007/BF02428029. [DOI] [PubMed] [Google Scholar]
  40. Yoshikawa H., Kazami J., Yamashita S., Chibazakura T., Sone H., Kawamura F., Oda M., Isaka M., Kobayashi Y., Saito H. Revised assignment for the Bacillus subtilis spo0F gene and its homology with spo0A and with two Escherichia coli genes. Nucleic Acids Res. 1986 Jan 24;14(2):1063–1072. doi: 10.1093/nar/14.2.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Zuber P., Losick R. Use of a lacZ fusion to study the role of the spoO genes of Bacillus subtilis in developmental regulation. Cell. 1983 Nov;35(1):275–283. doi: 10.1016/0092-8674(83)90230-1. [DOI] [PubMed] [Google Scholar]
  42. Zuber P., Losick R. spoOH: a developmental regulatory gene for promoter utilization in Bacillus subtilis. Cold Spring Harb Symp Quant Biol. 1985;50:483–488. doi: 10.1101/sqb.1985.050.01.060. [DOI] [PubMed] [Google Scholar]

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