Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 Jun;169(6):2417–2423. doi: 10.1128/jb.169.6.2417-2423.1987

Regulation of expression of pectate lyase genes pelA, pelD, and pelE in Erwinia chrysanthemi.

S Reverchon, J Robert-Baudouy
PMCID: PMC212080  PMID: 3108234

Abstract

The regulation of pelA, pelD, and pelE genes encoding three of the five major pectate lyase isoenzymes (PLa, PLd, and PLe) in Erwinia chrysanthemi B374 was analyzed by using genetic fusions to lacZ. These three genes are clustered on a 5-kilobase DNA fragment in the order pelD-pelE-pelA and constitute three independent transcriptional units. We localized the pelDEA cluster near the pro-1 marker on the genetic map of B374 by chromosomal mobilization with RP4::mini-Mu plasmid pULB110. Three classes of regulatory mutations responsible for constitutive pectate lyase synthesis have been described (kdgR, gpiR, and cri). We studied the effects of each mutation on pelE, pelD, and pelA expression independently. The mutations kdgR and gpiR mainly affect the expression of pelE and pelD, although PLa synthesis is slightly increased. The cri mutation results in a low level of constitutive expression of the three pel genes, but it is a pleiotropic mutation since other genes not involved in pectinolysis are also affected. In addition, we demonstrated that exuR, a negative regulatory gene governing the catabolism of hexuronates, does not modify the expression of pel genes. The frequency of gpiR or cri mutations (about 10(-8)) and the resulting constitutivity of pectate lyase synthesis suggest that these genes act as negative regulatory genes in addition to kdgR, which is already known to encode a repressor. Moreover, we found that expression of pel-lac fusions carried on pBR322 derivatives was higher in E. chrysanthemi than in Escherichia coli; this fact suggests the existence of positive regulation of pectate lyase synthesis in E. chrysanthemi.

Full text

PDF
2417

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bertheau Y., Madgidi-Hervan E., Kotoujansky A., Nguyen-The C., Andro T., Coleno A. Detection of depolymerase isoenzymes after electrophoresis or electrofocusing, or in titration curves. Anal Biochem. 1984 Jun;139(2):383–389. doi: 10.1016/0003-2697(84)90022-8. [DOI] [PubMed] [Google Scholar]
  3. Castilho B. A., Olfson P., Casadaban M. J. Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons. J Bacteriol. 1984 May;158(2):488–495. doi: 10.1128/jb.158.2.488-495.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chatterjee A. K., Thurn K. K., Tyrell D. J. Isolation and characterization of Tn5 insertion mutants of Erwinia chrysanthemi that are deficient in polygalacturonate catabolic enzymes oligogalacturonate lyase and 3-deoxy-D-glycero-2,5-hexodiulosonate dehydrogenase. J Bacteriol. 1985 May;162(2):708–714. doi: 10.1128/jb.162.2.708-714.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collmer A., Bateman D. F. Impaired induction and self-catabolite repression of extracellular pectate lyase in Erwinia chrysanthemi mutants deficient in oligogalacturonide lyase. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3920–3924. doi: 10.1073/pnas.78.6.3920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Condemine G., Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. Isolation of Erwinia chrysanthemi kduD mutants altered in pectin degradation. J Bacteriol. 1986 Mar;165(3):937–941. doi: 10.1128/jb.165.3.937-941.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gijsegem F., Toussaint A., Schoonejans E. In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi. EMBO J. 1985 Mar;4(3):787–792. doi: 10.1002/j.1460-2075.1985.tb03698.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HAMON Y., PERON Y. [The reciprocal antagonistic properties among the Erwinia. Discussion of the taxonomic position of this genus]. C R Hebd Seances Acad Sci. 1961 Jul 31;253:913–915. [PubMed] [Google Scholar]
  9. Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. Hexuronate catabolism in Erwinia chrysanthemi. J Bacteriol. 1987 Mar;169(3):1223–1231. doi: 10.1128/jb.169.3.1223-1231.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. Lactose metabolism in Erwinia chrysanthemi. J Bacteriol. 1985 Apr;162(1):248–255. doi: 10.1128/jb.162.1.248-255.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moran F., Nasuno S., Starr M. P. Extracellular and intracellular polygllacturonic acid trans-eliminases of Erwinia carotovora. Arch Biochem Biophys. 1968 Feb;123(2):298–306. doi: 10.1016/0003-9861(68)90138-0. [DOI] [PubMed] [Google Scholar]
  12. Moran F., Starr M. P. Metabolic regulation of polygalacturonic acid trans-eliminase in Erwinia. Eur J Biochem. 1969 Dec;11(2):291–295. doi: 10.1111/j.1432-1033.1969.tb00772.x. [DOI] [PubMed] [Google Scholar]
  13. Reverchon S., Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. Cloning of genes encoding pectolytic enzymes from a genomic library of the phytopathogenic bacterium, Erwinia chrysanthemi. Gene. 1985;35(1-2):121–130. doi: 10.1016/0378-1119(85)90164-7. [DOI] [PubMed] [Google Scholar]
  14. Reverchon S., Van Gijsegem F., Rouve M., Kotoujansky A., Robert-Baudouy J. Organization of a pectate lyase gene family in Erwinia chrysanthemi. Gene. 1986;49(2):215–224. doi: 10.1016/0378-1119(86)90282-9. [DOI] [PubMed] [Google Scholar]
  15. Schoonejans E., Toussaint A. Utilization of plasmid pULB113 (RP4::mini-Mu) to construct a linkage map of Erwinia carotovora subsp. chrysanthemi. J Bacteriol. 1983 Jun;154(3):1489–1492. doi: 10.1128/jb.154.3.1489-1492.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tsuyumu S. "Self-catabolite repression" of pectate lyase in Erwinia carotovora. J Bacteriol. 1979 Feb;137(2):1035–1036. doi: 10.1128/jb.137.2.1035-1036.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Van Gijsegem F., Toussaint A. Chromosome transfer and R-prime formation by an RP4::mini-Mu derivative in Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae, and Proteus mirabilis. Plasmid. 1982 Jan;7(1):30–44. doi: 10.1016/0147-619x(82)90024-5. [DOI] [PubMed] [Google Scholar]
  18. Zink R. T., Engwall J. K., McEvoy J. L., Chatterjee A. K. recA is required in the induction of pectin lyase and carotovoricin in Erwinia carotovora subsp. carotovora. J Bacteriol. 1985 Oct;164(1):390–396. doi: 10.1128/jb.164.1.390-396.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. van Gijsegem F., Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. Isolation and characterization of Erwinia chrysanthemi mutants defective in degradation of hexuronates. J Bacteriol. 1985 Feb;161(2):702–708. doi: 10.1128/jb.161.2.702-708.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES