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. 1987 Jul;169(7):2945–2949. doi: 10.1128/jb.169.7.2945-2949.1987

Altered translation of the uncC gene coding for the epsilon subunit of the F1F0-ATPase of Escherichia coli.

G B Cox, D Webb, L Hatch, R Lightowlers, A Munn, F Gibson
PMCID: PMC212331  PMID: 2885305

Abstract

The nucleotide sequence of the previously described uncC424 allele was determined and found to be the same as that of a wild-type uncC gene. However, a G----A change occurred 7 nucleotides upstream from the translation start codon, changing the putative Shine-Dalgarno sequence from GAGG to GAAG. Four revertant strains were examined. In one revertant, which had normal growth and membrane properties, a single base deletion had occurred to re-form the Shine-Dalgarno sequence GAGG 1 nucleotide closer to the translation start codon. A second revertant had a single base deletion in the preceding uncD gene, causing an extension of the beta subunit by 6 amino acids and an increase, presumably by translational coupling, in the amount of epsilon subunit. The third and fourth revertant strains were phenotypically similar and had either C----T or G----T changes 18 or 19 nucleotides, respectively, upstream from the translation start codon.

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Selected References

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