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. 1987 Jul;169(7):3369–3371. doi: 10.1128/jb.169.7.3369-3371.1987

Reexamination of the presence and linkage of 3-hydroxybutyryl substituents in the acidic capsular polysaccharide of Rhizobium trifolii 0403.

R I Hollingsworth, F B Dazzo, A J Mort
PMCID: PMC212394  PMID: 3597324

Abstract

We resolved previous conflicting results concerning the presence of 3-hydroxybutyryl substituents on the extracellular acidic polysaccharide from Rhizobium trifolii 0403. These substituents were indeed present in the polysaccharide and in the oligosaccharide fragments obtained by hydrogen fluoride solvolysis of the extracellular and capsular polysaccharides of the bacteria grown on plates. The 3-hydroxybutyrate substituent could be removed from the polysaccharide by 10 mM sodium deuteroxide without evidence of elimination, indicating that this substituent was ester linked.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe M., Sherwood J. E., Hollingsworth R. I., Dazzo F. B. Stimulation of clover root hair infection by lectin-binding oligosaccharides from the capsular and extracellular polysaccharides of Rhizobium trifolii. J Bacteriol. 1984 Nov;160(2):517–520. doi: 10.1128/jb.160.2.517-520.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bhuvaneswari T. V., Bauer W. D. Role of Lectins in Plant-Microorganism Interactions: III. Influence of Rhizosphere/Rhizoplane Culture Conditions on the Soybean Lectin-binding Properties of Rhizobia. Plant Physiol. 1978 Jul;62(1):71–74. doi: 10.1104/pp.62.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hollingsworth R. I., Abe M., Sherwood J. E., Dazzo F. B. Bacteriophage-induced acidic heteropolysaccharide lyases that convert the acidic heteropolysaccharides of Rhizobium trifolii into oligosaccharide units. J Bacteriol. 1984 Nov;160(2):510–516. doi: 10.1128/jb.160.2.510-516.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hrabak E. M., Urbano M. R., Dazzo F. B. Growth-phase-dependent immunodeterminants of Rhizobium trifolii lipopolysaccharide which bind trifoliin A, a white clover lectin. J Bacteriol. 1981 Nov;148(2):697–711. doi: 10.1128/jb.148.2.697-711.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kuo M. S., Mort A. J. Location and identity of the acyl substituents on the extracellular polysaccharides of Rhizobium trifolii and Rhizobium leguminosarum. Carbohydr Res. 1986 Jan 1;145(2):247–265. doi: 10.1016/s0008-6215(00)90433-6. [DOI] [PubMed] [Google Scholar]
  6. Mort A. J., Bauer W. D. Composition of the Capsular and Extracellular Polysaccharides of Rhizobium japonicum: CHANGES WITH CULTURE AGE AND CORRELATIONS WITH BINDING OF SOYBEAN SEED LECTIN TO THE BACTERIA . Plant Physiol. 1980 Jul;66(1):158–163. doi: 10.1104/pp.66.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]

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