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. 1987 Jul;169(7):3375–3378. doi: 10.1128/jb.169.7.3375-3378.1987

Similarities in control of mini-F plasmid and chromosomal replication in Escherichia coli.

M S Shields, B C Kline, J E Tam
PMCID: PMC212396  PMID: 3298219

Abstract

In Escherichia coli, concentrations of a mini-F plasmid with two origins of replication (oriV and oriS) were 50% lower in fast-growing cells than in slow-growing cells. By contrast, a mini-F plasmid deleted for oriV maintained a uniform concentration in both fast- and slow-growing cells, and in this behavior the plasmid mimicked the control by the host of chromosomal origin (oriC) concentration.

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Selected References

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  1. Abeles A. L., Snyder K. M., Chattoraj D. K. P1 plasmid replication: replicon structure. J Mol Biol. 1984 Mar 5;173(3):307–324. doi: 10.1016/0022-2836(84)90123-2. [DOI] [PubMed] [Google Scholar]
  2. Braun R. E., O'Day K., Wright A. Autoregulation of the DNA replication gene dnaA in E. coli K-12. Cell. 1985 Jan;40(1):159–169. doi: 10.1016/0092-8674(85)90319-8. [DOI] [PubMed] [Google Scholar]
  3. Churchward G., Linder P., Caro L. The nucleotide sequence of replication and maintenance functions encoded by plasmid pSC101. Nucleic Acids Res. 1983 Aug 25;11(16):5645–5659. doi: 10.1093/nar/11.16.5645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Collins J., Pritchard R. H. Relationship between chromosome replication and F'lac episome replication in Escherichia coli. J Mol Biol. 1973 Jun 25;78(1):143–155. doi: 10.1016/0022-2836(73)90434-8. [DOI] [PubMed] [Google Scholar]
  5. Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
  6. Donachie W. D. Relationship between cell size and time of initiation of DNA replication. Nature. 1968 Sep 7;219(5158):1077–1079. doi: 10.1038/2191077a0. [DOI] [PubMed] [Google Scholar]
  7. Eichenlaub R., Figurski D., Helinski D. R. Bidirection replication from a unique origin in a mini-F plasmid. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1138–1141. doi: 10.1073/pnas.74.3.1138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Frame R., Bishop J. O. The number of sex-factors per chromosome in Escherichia coli. Biochem J. 1971 Jan;121(1):93–103. doi: 10.1042/bj1210093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fuller R. S., Funnell B. E., Kornberg A. The dnaA protein complex with the E. coli chromosomal replication origin (oriC) and other DNA sites. Cell. 1984 Oct;38(3):889–900. doi: 10.1016/0092-8674(84)90284-8. [DOI] [PubMed] [Google Scholar]
  10. Kamio Y., Terawaki Y. Nucleotide sequence of an incompatibility region of mini-Rts1 that contains five direct repeats. J Bacteriol. 1983 Sep;155(3):1185–1191. doi: 10.1128/jb.155.3.1185-1191.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kim K., Meyer R. J. Copy number of the broad host-range plasmid R1162 is determined by the amounts of essential plasmid-encoded proteins. J Mol Biol. 1985 Oct 20;185(4):755–767. doi: 10.1016/0022-2836(85)90060-9. [DOI] [PubMed] [Google Scholar]
  12. Kline B. C. A review of mini-F plasmid maintenance. Plasmid. 1985 Jul;14(1):1–16. doi: 10.1016/0147-619x(85)90027-7. [DOI] [PubMed] [Google Scholar]
  13. Kline B. C., Palchaudhuri S. Genetic studies of F plasmid maintenance genes. Plasmid. 1980 Nov;4(3):281–291. doi: 10.1016/0147-619x(80)90067-0. [DOI] [PubMed] [Google Scholar]
  14. Kline B. C., Trawick J. Identification and characterization of a second copy number control gene in mini-F plasmids. Mol Gen Genet. 1983;192(3):408–415. doi: 10.1007/BF00392183. [DOI] [PubMed] [Google Scholar]
  15. Lane D., Gardner R. C. Second EcoRI fragment of F capable of self-replication. J Bacteriol. 1979 Jul;139(1):141–151. doi: 10.1128/jb.139.1.141-151.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lane H. E. Replication and incompatibility of F and plasmids in the IncFI Group. Plasmid. 1981 Jan;5(1):100–126. doi: 10.1016/0147-619x(81)90079-2. [DOI] [PubMed] [Google Scholar]
  17. Linder P., Churchward G., Xia G. X., Yu Y. Y., Caro L. An essential replication gene, repA, of plasmid pSC101 is autoregulated. J Mol Biol. 1985 Feb 5;181(3):383–393. doi: 10.1016/0022-2836(85)90227-x. [DOI] [PubMed] [Google Scholar]
  18. Lovett M. A., Helinski D. R. Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid. J Bacteriol. 1976 Aug;127(2):982–987. doi: 10.1128/jb.127.2.982-987.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Manis J. J., Kline B. C. Restriction endonuclease mapping and mutagenesis of the F sex factor replication region. Mol Gen Genet. 1977 Apr 29;152(3):175–182. doi: 10.1007/BF00268815. [DOI] [PubMed] [Google Scholar]
  20. Masson L., Ray D. S. Mechanism of autonomous control of the Escherichia coli F plasmid: different complexes of the initiator/repressor protein are bound to its operator and to an F plasmid replication origin. Nucleic Acids Res. 1986 Jul 25;14(14):5693–5711. doi: 10.1093/nar/14.14.5693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. McKnight S., Tjian R. Transcriptional selectivity of viral genes in mammalian cells. Cell. 1986 Sep 12;46(6):795–805. doi: 10.1016/0092-8674(86)90061-9. [DOI] [PubMed] [Google Scholar]
  22. Murotsu T., Matsubara K., Sugisaki H., Takanami M. Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid. Gene. 1981 Nov;15(2-3):257–271. doi: 10.1016/0378-1119(81)90135-9. [DOI] [PubMed] [Google Scholar]
  23. Pritchard R. H., Chandler M. G., Collins J. Independence of F replication and chromosome replication in Escherichia coli. Mol Gen Genet. 1975;138(2):143–155. doi: 10.1007/BF02428118. [DOI] [PubMed] [Google Scholar]
  24. Søgaard-Andersen L., Rokeach L. A., Molin S. Regulated expression of a gene important for replication of plasmid F in E. coli. EMBO J. 1984 Feb;3(2):257–262. doi: 10.1002/j.1460-2075.1984.tb01794.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tanimoto K., Iino T. An essential gene for replication of the mini-F plasmid from origin I. Mol Gen Genet. 1984;196(1):59–63. doi: 10.1007/BF00334092. [DOI] [PubMed] [Google Scholar]
  26. Timmis K., Cabello F., Cohen S. N. Cloning, isolation, and characterization of replication regions of complex plasmid genomes. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2242–2246. doi: 10.1073/pnas.72.6.2242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tokino T., Murotsu T., Matsubara K. Purification and properties of the mini-F plasmid-encoded E protein needed for autonomous replication control of the plasmid. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4109–4113. doi: 10.1073/pnas.83.12.4109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tolun A., Helinski D. R. Direct repeats of the F plasmid incC region express F incompatibility. Cell. 1981 Jun;24(3):687–694. doi: 10.1016/0092-8674(81)90095-7. [DOI] [PubMed] [Google Scholar]
  29. Trawick J. D., Kline B. C. A two-stage molecular model for control of mini-F replication. Plasmid. 1985 Jan;13(1):59–69. doi: 10.1016/0147-619x(85)90056-3. [DOI] [PubMed] [Google Scholar]
  30. Tsutsui H., Fujiyama A., Murotsu T., Matsubara K. Role of nine repeating sequences of the mini-F genome for expression of F-specific incompatibility phenotype and copy number control. J Bacteriol. 1983 Jul;155(1):337–344. doi: 10.1128/jb.155.1.337-344.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Vocke C., Bastia D. Primary structure of the essential replicon of the plasmid pSC101. Proc Natl Acad Sci U S A. 1983 Nov;80(21):6557–6561. doi: 10.1073/pnas.80.21.6557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Vocke C., Bastia D. The replication initiator protein of plasmid pSC101 is a transcriptional repressor of its own cistron. Proc Natl Acad Sci U S A. 1985 Apr;82(8):2252–2256. doi: 10.1073/pnas.82.8.2252. [DOI] [PMC free article] [PubMed] [Google Scholar]

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