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. 1987 Jul;169(7):3379–3384. doi: 10.1128/jb.169.7.3379-3384.1987

Overexpression and DNA-binding properties of the mer-encoded regulatory protein from plasmid NR1 (Tn21).

A Heltzel, D Gambill, W J Jackson, P A Totis, A O Summers
PMCID: PMC212397  PMID: 3036786

Abstract

In plasmid NR1 the expression of genes involved in mercury resistance (Tn21) is regulated by the trans-acting product of the merR gene. An in vivo T7 RNA polymerase-promoter overexpression system was used to detect a protein of approximately 16,000 daltons encoded by the merR reading frame. Overexpressed MerR constituted about 5% of labeled proteins. An in vitro MerR-mer-op (mer-op is the mer operator and promoter region) gel electrophoresis binding assay established that the binding site for MerR was located between the putative -35 and -10 sequences of the promoter for the mer structural genes. A nonsense mutation in the carboxyl half of MerR resulted in the loss of biological function and the loss of in vitro mer-op binding properties.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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