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. 1987 Aug;169(8):3435–3440. doi: 10.1128/jb.169.8.3435-3440.1987

Regulation of the Escherichia coli uvrD gene in vivo.

H M Arthur, D R Cavanagh, P W Finch, P T Emmerson
PMCID: PMC212414  PMID: 3038838

Abstract

The roles of two putative promoter sequences, P1 and P2, and a potential antiterminator sequence found in the uvrD control region were examined in vivo. Constitutive and SOS-induced levels of uvrD mRNA were determined by S1 mapping, and it was shown that the majority of uvrD transcripts are from P1, while P2 plays only a minor role. A series of increasing deletions from the 5' end of the uvrD gene was used to assay transcription in the promoterless vector pKO-1. Loss of just the -35 region of P1 was sufficient to switch off detectable transcription from both P1 and P2. Disruption of the antiterminator by site-specific mutagenesis had no effect on constitutive levels of transcription, but led to a significant increase over wild-type levels following SOS induction. This suggests that the attenuator comes into play following DNA damage to moderate the increase in UvrD protein synthesis.

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Selected References

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