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. 1987 Aug;169(8):3521–3524. doi: 10.1128/jb.169.8.3521-3524.1987

Transcriptional regulation of the DAL5 gene in Saccharomyces cerevisiae.

R Rai, F Genbauffe, H Z Lea, T G Cooper
PMCID: PMC212427  PMID: 3301804

Abstract

We demonstrate that the DAL5 gene, encoding a necessary component of the allantoate transport system, is constitutively expressed in Saccharomyces cerevisiae. Its relatively high basal level of expression did not increase further upon addition of allantoin pathway intermediates. However, steady-state DAL5 mRNA levels dropped precipitously when a repressive nitrogen source was provided. These control characteristics of DAL5 expression make this gene a good model with which to unravel the mechanism of nitrogen catabolite repression. Its particular advantage relative to other potentially useful genes derives from its lack of control by induction and hence the complicating effects of inducer exclusion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bossinger J., Cooper T. G. Execution times of macromolecular synthetic processes involved in the induction of allophanate hydrolase at 15 degrees C. J Bacteriol. 1976 Oct;128(1):498–501. doi: 10.1128/jb.128.1.498-501.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bossinger J., Cooper T. G. Molecular events associated with induction of arginase in Saccharomyces cerevisiae. J Bacteriol. 1977 Jul;131(1):163–173. doi: 10.1128/jb.131.1.163-173.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bossinger J., Cooper T. G. Sequence of molecular events involved in induction of allophanate hydrolase. J Bacteriol. 1976 Apr;126(1):198–204. doi: 10.1128/jb.126.1.198-204.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brandriss M. C., Magasanik B. Genetics and physiology of proline utilization in Saccharomyces cerevisiae: enzyme induction by proline. J Bacteriol. 1979 Nov;140(2):498–503. doi: 10.1128/jb.140.2.498-503.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brandriss M. C., Magasanik B. Genetics and physiology of proline utilization in Saccharomyces cerevisiae: mutation causing constitutive enzyme expression. J Bacteriol. 1979 Nov;140(2):504–507. doi: 10.1128/jb.140.2.504-507.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brandriss M. C. Proline utilization in Saccharomyces cerevisiae: analysis of the cloned PUT2 gene. Mol Cell Biol. 1983 Oct;3(10):1846–1856. doi: 10.1128/mcb.3.10.1846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chisholm G., Cooper T. G. Isolation and characterization of mutants that produce the allantoin-degrading enzymes constitutively in Saccharomyces cerevisiae. Mol Cell Biol. 1982 Sep;2(9):1088–1095. doi: 10.1128/mcb.2.9.1088. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Chisholm V. T., Lea H. Z., Rai R., Cooper T. G. Regulation of allantoate transport in wild-type and mutant strains of Saccharomyces cerevisiae. J Bacteriol. 1987 Apr;169(4):1684–1690. doi: 10.1128/jb.169.4.1684-1690.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dunlop P. C., Meyer G. M., Roon R. J. Nitrogen catabolite repression of asparaginase II in Saccharomyces cerevisiae. J Bacteriol. 1980 Jul;143(1):422–426. doi: 10.1128/jb.143.1.422-426.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Genbauffe F. S., Cooper T. G. Induction and repression of the urea amidolyase gene in Saccharomyces cerevisiae. Mol Cell Biol. 1986 Nov;6(11):3954–3964. doi: 10.1128/mcb.6.11.3954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kang L., Keeler M. L., Dunlop P. C., Roon R. J. Nitrogen catabolite repression in a glutamate auxotroph of Saccharomyces cerevisiae. J Bacteriol. 1982 Jul;151(1):29–35. doi: 10.1128/jb.151.1.29-35.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lawther R. P., Cooper T. G. Kinetics of induced and repressed enzyme synthesis in Saccharomyces cerevisiae. J Bacteriol. 1975 Mar;121(3):1064–1073. doi: 10.1128/jb.121.3.1064-1073.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lawther R. P., Phillips S. L., Cooper T. G. Lomofungin inhibition of allophanate hydrolase synthesis in Saccharomyces cerevisiae. Mol Gen Genet. 1975;137(2):89–99. doi: 10.1007/BF00341675. [DOI] [PubMed] [Google Scholar]
  14. Mitchell A. P., Magasanik B. Regulation of glutamine-repressible gene products by the GLN3 function in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Dec;4(12):2758–2766. doi: 10.1128/mcb.4.12.2758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mitchell A. P., Magasanik B. Three regulatory systems control production of glutamine synthetase in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Dec;4(12):2767–2773. doi: 10.1128/mcb.4.12.2767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nasmyth K. A., Reed S. I. Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2119–2123. doi: 10.1073/pnas.77.4.2119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Struhl K., Stinchcomb D. T., Scherer S., Davis R. W. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035–1039. doi: 10.1073/pnas.76.3.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sumrada R. A., Cooper T. G. Isolation of the CAR1 gene from Saccharomyces cerevisiae and analysis of its expression. Mol Cell Biol. 1982 Dec;2(12):1514–1523. doi: 10.1128/mcb.2.12.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sumrada R. A., Cooper T. G. Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast. J Biol Chem. 1982 Aug 10;257(15):9119–9127. [PubMed] [Google Scholar]
  20. Turoscy V., Cooper T. G. Allantoate transport in Saccharomyces cerevisiae. J Bacteriol. 1979 Dec;140(3):971–979. doi: 10.1128/jb.140.3.971-979.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Turoscy V., Cooper T. G. Pleiotropic control of five eucaryotic genes by multiple regulatory elements. J Bacteriol. 1982 Sep;151(3):1237–1246. doi: 10.1128/jb.151.3.1237-1246.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yoo H. S., Genbauffe F. S., Cooper T. G. Identification of the ureidoglycolate hydrolase gene in the DAL gene cluster of Saccharomyces cerevisiae. Mol Cell Biol. 1985 Sep;5(9):2279–2288. doi: 10.1128/mcb.5.9.2279. [DOI] [PMC free article] [PubMed] [Google Scholar]

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