Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 Aug;169(8):3685–3690. doi: 10.1128/jb.169.8.3685-3690.1987

Organization of phosphoribulokinase and ribulose bisphosphate carboxylase/oxygenase genes in Rhodopseudomonas (Rhodobacter) sphaeroides.

J L Gibson, F R Tabita
PMCID: PMC212451  PMID: 3038848

Abstract

A heterologous phosphoribulokinase (PRK) gene probe was used to analyze two recombinant plasmids isolated from a Rhodopseudomonas (Rhodobacter) sphaeroides gene library. These plasmids were previously shown to carry the genes for form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBPC/O). Southern blot hybridization analysis indicated that there were two PRK genes linked to the RuBPC/O coding sequences. Restriction mapping showed the arrangement of the duplicate sets of PRK and RuBPC/O to be distinct. Subcloning of the hybridizing PRK sequences downstream of the lac promoter of pUC8 allowed expression of the two PRK enzymes in Escherichia coli. Analysis of the purified proteins by sodium dodecyl sulfate-slab gel electrophoresis revealed polypeptides with molecular weights of 32,000 and 34,000 corresponding to the form I and form II PRKs, respectively. Preliminary experiments on sensitivity to NADH regulation suggested that the two PRK enzymes differ in catalytic properties.

Full text

PDF
3687

Images in this article

3687Image
on p.3687
3689Image
on p.3689

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdelal A. T., Schlegel H. G. Purification and regulatory properties of phosphoribulokinase from Hydrogenomonas eutropha H 16. Biochem J. 1974 Jun;139(3):481–489. doi: 10.1042/bj1390481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyer H. W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969 May 14;41(3):459–472. doi: 10.1016/0022-2836(69)90288-5. [DOI] [PubMed] [Google Scholar]
  3. Gibson J. L., Tabita F. R. Different molecular forms of D-ribulose-1,5-bisphosphate carboxylase from Rhodopseudomonas sphaeroides. J Biol Chem. 1977 Feb 10;252(3):943–949. [PubMed] [Google Scholar]
  4. Gibson J. L., Tabita F. R. Isolation and preliminary characterization of two forms of ribulose 1,5-bisphosphate carboxylase from Rhodopseudomonas capsulata. J Bacteriol. 1977 Dec;132(3):818–823. doi: 10.1128/jb.132.3.818-823.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gibson J. L., Tabita F. R. Isolation of the Rhodopseudomonas sphaeroides form I ribulose 1,5-bisphosphate carboxylase/oxygenase large and small subunit genes and expression of the active hexadecameric enzyme in Escherichia coli. Gene. 1986;44(2-3):271–278. doi: 10.1016/0378-1119(86)90191-5. [DOI] [PubMed] [Google Scholar]
  6. Gibson J. L., Tabita F. R. Structural differences in the catalytic subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase from Rhodopseudomonas sphaeroides. J Bacteriol. 1985 Dec;164(3):1188–1193. doi: 10.1128/jb.164.3.1188-1193.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jouanneau Y., Tabita F. R. Independent regulation of synthesis of form I and form II ribulose bisphosphate carboxylase-oxygenase in Rhodopseudomonas sphaeroides. J Bacteriol. 1986 Feb;165(2):620–624. doi: 10.1128/jb.165.2.620-624.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kiesow L. A., Lindsley B. F., Bless J. W. Phosphoribulokinase from Nitrobacter winogradskyi: activation by reduced nicotinamide adenine dinucleotide and inhibition by pyridoxal phosphate. J Bacteriol. 1977 Apr;130(1):20–25. doi: 10.1128/jb.130.1.20-25.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klintworth R., Husemann M., Salnikow J., Bowien B. Chromosomal and plasmid locations for phosphoribulokinase genes in Alcaligenes eutrophus. J Bacteriol. 1985 Nov;164(2):954–956. doi: 10.1128/jb.164.2.954-956.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Knauf V. C., Nester E. W. Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium Ti plasmid. Plasmid. 1982 Jul;8(1):45–54. doi: 10.1016/0147-619x(82)90040-3. [DOI] [PubMed] [Google Scholar]
  12. Lugtenberg B., Meijers J., Peters R., van der Hoek P., van Alphen L. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands. FEBS Lett. 1975 Oct 15;58(1):254–258. doi: 10.1016/0014-5793(75)80272-9. [DOI] [PubMed] [Google Scholar]
  13. MacElroy R. D., Johnson E. J., Johnson M. K. Control of ATP-dependent CO2 fixation in extracts of Hydrogenomonas facilis: NADH regulation of phosphoribulokinase. Arch Biochem Biophys. 1969 Apr;131(1):272–275. doi: 10.1016/0003-9861(69)90131-3. [DOI] [PubMed] [Google Scholar]
  14. Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
  15. Muller E. D., Chory J., Kaplan S. Cloning and characterization of the gene product of the form II ribulose-1,5-bisphosphate carboxylase gene of Rhodopseudomonas sphaeroides. J Bacteriol. 1985 Jan;161(1):469–472. doi: 10.1128/jb.161.1.469-472.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Quivey R. G., Jr, Tabita F. R. Cloning and expression in Escherichia coli of the form II ribulose 1,5-bisphosphate carboxylase/oxygenase gene from Rhodopseudomonas sphaeroides. Gene. 1984 Nov;31(1-3):91–101. doi: 10.1016/0378-1119(84)90198-7. [DOI] [PubMed] [Google Scholar]
  17. Rindt K. P., Ohmann E. NADH and AMP as allosteric effectors of ribulose-5-phosphate kinase in Rhodopseudomonas spheroides. Biochem Biophys Res Commun. 1969 Aug 7;36(3):357–364. doi: 10.1016/0006-291x(69)90572-5. [DOI] [PubMed] [Google Scholar]
  18. Shively J. M., Davidson E., Marrs B. L. Depression of the synthesis of the intermediate and large forms of ribulose-1,5-bisphosphate carboxylase/oxygenase in Rhodopseudomonas capsulata. Arch Microbiol. 1984 Jul;138(3):233–236. doi: 10.1007/BF00402127. [DOI] [PubMed] [Google Scholar]
  19. Siebert K., Schobert P., Bowien B. Purification, some catalytic and molecular properties of phosphoribulokinase from Alcaligenes eutrophus. Biochim Biophys Acta. 1981 Mar 13;658(1):35–44. doi: 10.1016/0005-2744(81)90247-3. [DOI] [PubMed] [Google Scholar]
  20. Tabita F. R. Pyridine nucleotide control and subunit structure of phosphoribulokinase from photosynthetic bacteria. J Bacteriol. 1980 Sep;143(3):1275–1280. doi: 10.1128/jb.143.3.1275-1280.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  22. Weaver K. E., Tabita F. R. Complementation of a Rhodopseudomonas sphaeroides ribulose bisphosphate carboxylase-oxygenase regulatory mutant from a genomic library. J Bacteriol. 1985 Oct;164(1):147–154. doi: 10.1128/jb.164.1.147-154.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES