Abstract
Auxotrophs isolated from two chloramphenicol-nonproducing mutants of Streptomyces venezuelae included three requiring pyridoxal (Pxl-), VS248 (cml-11 pdx-2), VS253 (cml-11 pdx-3), and VS258 (cml-12 pdx-4), and one requiring thiosulfate, VS263 (cml-12 cys-28). Results of SV1-mediated transductions were consistent with the relative marker order cys-28-cml-12-cml-11-pdx-2,3,4,5, all of which were cotransducible and must therefore span less than 45 kilobases of DNA, the approximate length of DNA packaged by SV1. cys-28 was also cotransducible with arg-4 and arg-6, but arg and pdx were not cotransducible. Results of crosses with donors carrying any one of 11 cml mutations were consistent with the location of all cml mutations between cys-28 and pdx markers. Also, a new Pxl- auxotroph (pdx-6) and two new Cml- mutants were recovered after localized hydroxylamine mutagenesis of a cys-28 cml+ strain derived from VS263 by transduction.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Doull J. L., Vats S., Chaliciopoulos M., Stuttard C., Wong K., Vining L. C. Conjugational fertility and location of chloramphenicol biosynthesis genes on the chromosomal linkage map of Streptomyces venezuelae. J Gen Microbiol. 1986 May;132(5):1327–1338. doi: 10.1099/00221287-132-5-1327. [DOI] [PubMed] [Google Scholar]
- Doull J., Ahmed Z., Stuttard C., Vining L. C. Isolation and characterization of Streptomyces venezuelae mutants blocked in chloramphenicol biosynthesis. J Gen Microbiol. 1985 Jan;131(1):97–104. doi: 10.1099/00221287-131-1-97. [DOI] [PubMed] [Google Scholar]
- PERLMAN D., LANGLYKKE A. F., ROTHBERG H. D., Jr Observations on the chemical inhibition of Streptomyces griseus bacteriophage multiplication. J Bacteriol. 1951 Feb;61(2):135–143. doi: 10.1128/jb.61.2.135-143.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stuttard C., Atkinson L., Vats S. Genome structure in Streptomyces spp.: adjacent genes on the S. coelicolor A3(2) linkage map have cotransducible analogs in S. venezuelae. J Bacteriol. 1987 Aug;169(8):3814–3816. doi: 10.1128/jb.169.8.3814-3816.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stuttard C. Localized hydroxylamine mutagenesis, and cotransduction of threonine and lysine genes, in Streptomyces venezuelae. J Bacteriol. 1983 Sep;155(3):1219–1223. doi: 10.1128/jb.155.3.1219-1223.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]