Abstract
The F420 hydrogenase of Methanobacterium formicicum was associated with membranes isolated by sucrose density gradient ultracentrifugation of cell extract. The methyl viologen hydrogenase was present in the soluble fractions. Column chromatography with phenyl-Sepharose CL-4B revealed that the F420 hydrogenase was strongly hydrophobic, suggesting that it associates with isolated membranes through hydrophobic interactions.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blaut M., Gottschalk G. Coupling of ATP synthesis and methane formation from methanol and molecular hydrogen in Methanosarcina barkeri. Eur J Biochem. 1984 May 15;141(1):217–222. doi: 10.1111/j.1432-1033.1984.tb08178.x. [DOI] [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
- Doddema H. J., van der Drift C., Vogels G. D., Veenhuis M. Chemiosmotic coupling in Methanobacterium thermoautotrophicum: hydrogen-dependent adenosine 5'-triphosphate synthesis by subcellular particles. J Bacteriol. 1979 Dec;140(3):1081–1089. doi: 10.1128/jb.140.3.1081-1089.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jacobson F. S., Daniels L., Fox J. A., Walsh C. T., Orme-Johnson W. H. Purification and properties of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum. J Biol Chem. 1982 Apr 10;257(7):3385–3388. [PubMed] [Google Scholar]
- McKellar R. C., Sprott G. D. Solubilization and properties of a particulate hydrogenase from Methanobacterium strain G2R. J Bacteriol. 1979 Jul;139(1):231–238. doi: 10.1128/jb.139.1.231-238.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson M. J., Brown D. P., Ferry J. G. FAD requirement for the reduction of coenzyme F420 by hydrogenase from Methanobacterium formicicum. Biochem Biophys Res Commun. 1984 May 16;120(3):775–781. doi: 10.1016/s0006-291x(84)80174-6. [DOI] [PubMed] [Google Scholar]
- Nelson M. J., Ferry J. G. Carbon monoxide-dependent methyl coenzyme M methylreductase in acetotrophic Methosarcina spp. J Bacteriol. 1984 Nov;160(2):526–532. doi: 10.1128/jb.160.2.526-532.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schauer N. L., Ferry J. G. FAD requirement for the reduction of coenzyme F420 by formate dehydrogenase from Methanobacterium formicicum. J Bacteriol. 1983 Aug;155(2):467–472. doi: 10.1128/jb.155.2.467-472.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Terlesky K. C., Nelson M. J., Ferry J. G. Isolation of an enzyme complex with carbon monoxide dehydrogenase activity containing corrinoid and nickel from acetate-grown Methanosarcina thermophila. J Bacteriol. 1986 Dec;168(3):1053–1058. doi: 10.1128/jb.168.3.1053-1058.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wackett L. P., Hartwieg E. A., King J. A., Orme-Johnson W. H., Walsh C. T. Electron microscopy of nickel-containing methanogenic enzymes: methyl reductase and F420-reducing hydrogenase. J Bacteriol. 1987 Feb;169(2):718–727. doi: 10.1128/jb.169.2.718-727.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamazaki S. A selenium-containing hydrogenase from Methanococcus vannielii. Identification of the selenium moiety as a selenocysteine residue. J Biol Chem. 1982 Jul 25;257(14):7926–7929. [PubMed] [Google Scholar]