Abstract
Spent culture fluids from Aquaspirillum magnetotacticum MS-1 grown at high (20 microM) but not low (5 microM) iron concentration contained material yielding a positive hydroxamate test. Cells possessed six major outer membrane proteins. Three outer membrane proteins ranging from 72,000 to 85,000 daltons were coordinately produced at iron concentrations conducive to hydroxamate production. A 55,000-dalton iron-repressible outer membrane protein was also present in strain MS-1 cultured at low but not high ferric quinate concentration. Culture fluids from strain MS-1 which were hydroxamate positive augmented growth of a Salmonella typhimurium siderophore-deficient (enb-7) mutant in low-iron medium, suggesting a role of hydroxamate in uptake of iron by the cell.
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- Blakemore R. P. Magnetotactic bacteria. Annu Rev Microbiol. 1982;36:217–238. doi: 10.1146/annurev.mi.36.100182.001245. [DOI] [PubMed] [Google Scholar]
- Blakemore R. P., Maratea D., Wolfe R. S. Isolation and pure culture of a freshwater magnetic spirillum in chemically defined medium. J Bacteriol. 1979 Nov;140(2):720–729. doi: 10.1128/jb.140.2.720-729.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fekete F. A., Spence J. T., Emery T. Siderophores Produced by Nitrogen-Fixing Azotobacter vinelandii OP in Iron-Limited Continuous Culture. Appl Environ Microbiol. 1983 Dec;46(6):1297–1300. doi: 10.1128/aem.46.6.1297-1300.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holzberg M., Artis W. M. Hydroxamate siderophore production by opportunistic and systemic fungal pathogens. Infect Immun. 1983 Jun;40(3):1134–1139. doi: 10.1128/iai.40.3.1134-1139.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klebba P. E., McIntosh M. A., Neilands J. B. Kinetics of biosynthesis of iron-regulated membrane proteins in Escherichia coli. J Bacteriol. 1982 Mar;149(3):880–888. doi: 10.1128/jb.149.3.880-888.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Leong J., Neilands J. B. Mechanisms of siderophore iron transport in enteric bacteria. J Bacteriol. 1976 May;126(2):823–830. doi: 10.1128/jb.126.2.823-830.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luckey M., Pollack J. R., Wayne R., Ames B. N., Neilands J. B. Iron uptake in Salmonella typhimurium: utilization of exogenous siderochromes as iron carriers. J Bacteriol. 1972 Sep;111(3):731–738. doi: 10.1128/jb.111.3.731-738.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Messenger A. J., Ratledge C. Iron transport in Mycobacterium smegmatis: Uptake of iron from ferric citrate. J Bacteriol. 1982 Jan;149(1):131–135. doi: 10.1128/jb.149.1.131-135.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neilands J. B. Iron absorption and transport in microorganisms. Annu Rev Nutr. 1981;1:27–46. doi: 10.1146/annurev.nu.01.070181.000331. [DOI] [PubMed] [Google Scholar]
- Neilands J. B. Microbial envelope proteins related to iron. Annu Rev Microbiol. 1982;36:285–309. doi: 10.1146/annurev.mi.36.100182.001441. [DOI] [PubMed] [Google Scholar]
- Wagegg W., Braun V. Ferric citrate transport in Escherichia coli requires outer membrane receptor protein fecA. J Bacteriol. 1981 Jan;145(1):156–163. doi: 10.1128/jb.145.1.156-163.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]