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. 1986 Jul;167(1):168–173. doi: 10.1128/jb.167.1.168-173.1986

Cloning and nucleotide sequencing of genes for small, acid-soluble spore proteins of Bacillus cereus, Bacillus stearothermophilus, and "Thermoactinomyces thalpophilus".

C A Loshon, E R Fliss, B Setlow, H F Foerster, P Setlow
PMCID: PMC212856  PMID: 3087949

Abstract

As found previously with other Bacillus species, spores of B. stearothermophilus and "Thermoactinomyces thalpophilus" contained significant levels of small, acid-soluble spore proteins (SASP) which were rapidly degraded during spore germination and which reacted with antibodies raised against B. megaterium SASP. Genes coding for a B. stearothermophilus and a "T. thalpophilus" SASP as well as for two B. cereus SASP were cloned, their nucleotide sequences were determined, and the amino acid sequences of the SASP coded for were compared. Strikingly, all of the amino acid residues previously found to be conserved in this group of SASP both within and between two other Bacillus species (B. megaterium and B. subtilis) were also conserved in the SASP coded for by the B. cereus genes as well as those coded for by the genes from the more distantly related organisms B. stearothermophilus and "T. thalpophilus." This finding strongly suggests that there is significant selective pressure to conserve SASP primary sequence and thus that these proteins serve some function other than simply amino acid storage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Connors M. J., Mason J. M., Setlow P. Cloning and nucleotide sequencing of genes for three small, acid-soluble proteins from Bacillus subtilis spores. J Bacteriol. 1986 May;166(2):417–425. doi: 10.1128/jb.166.2.417-425.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Connors M. J., Setlow P. Cloning of a small, acid-soluble spore protein gene from Bacillus subtilis and determination of its complete nucleotide sequence. J Bacteriol. 1985 Jan;161(1):333–339. doi: 10.1128/jb.161.1.333-339.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cross T., Walker P. D., Gould G. W. Thermophilic actinomycetes producing resistant endospores. Nature. 1968 Oct 26;220(5165):352–354. doi: 10.1038/220352a0. [DOI] [PubMed] [Google Scholar]
  5. Fliss E. R., Loshon C. A., Setlow P. Genes for Bacillus megaterium small, acid-soluble spore proteins: cloning and nucleotide sequence of three additional genes from this multigene family. J Bacteriol. 1986 Feb;165(2):467–473. doi: 10.1128/jb.165.2.467-473.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fliss E. R., Setlow P. Bacillus megaterium spore protein C-3: nucleotide sequence of its gene and the amino acid sequence at its spore protease cleavage site. Gene. 1984 Oct;30(1-3):167–172. doi: 10.1016/0378-1119(84)90117-3. [DOI] [PubMed] [Google Scholar]
  7. Fliss E. R., Setlow P. Complete nucleotide sequence and start sites for transcription and translation of the Bacillus megaterium protein C gene. J Bacteriol. 1984 Jun;158(3):809–813. doi: 10.1128/jb.158.3.809-813.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fliss E. R., Setlow P. Genes for Bacillus megaterium small, acid-soluble spore proteins: nucleotide sequence of two genes and their expression during sporulation. Gene. 1985;35(1-2):151–157. doi: 10.1016/0378-1119(85)90167-2. [DOI] [PubMed] [Google Scholar]
  9. Foerster H. F. Activation and germination characteristics observed in endospores of thermophilic strains of Bacillus. Arch Microbiol. 1983 Jun;134(3):175–181. doi: 10.1007/BF00407754. [DOI] [PubMed] [Google Scholar]
  10. Fox G. E., Stackebrandt E., Hespell R. B., Gibson J., Maniloff J., Dyer T. A., Wolfe R. S., Balch W. E., Tanner R. S., Magrum L. J. The phylogeny of prokaryotes. Science. 1980 Jul 25;209(4455):457–463. doi: 10.1126/science.6771870. [DOI] [PubMed] [Google Scholar]
  11. Goldrick S., Setlow P. Expression of a Bacillus megaterium sporulation-specific gene during sporulation of Bacillus subtilis. J Bacteriol. 1983 Sep;155(3):1459–1462. doi: 10.1128/jb.155.3.1459-1462.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Johnson W. C., Tipper D. J. Acid-soluble spore proteins of Bacillus subtilis. J Bacteriol. 1981 Jun;146(3):972–982. doi: 10.1128/jb.146.3.972-982.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mason J. M., Setlow P. Essential role of small, acid-soluble spore proteins in resistance of Bacillus subtilis spores to UV light. J Bacteriol. 1986 Jul;167(1):174–178. doi: 10.1128/jb.167.1.174-178.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  15. Nakajima R., Imanaka T., Aiba S. Nucleotide sequence of the Bacillus stearothermophilus alpha-amylase gene. J Bacteriol. 1985 Jul;163(1):401–406. doi: 10.1128/jb.163.1.401-406.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. REISFELD R. A., LEWIS U. J., WILLIAMS D. E. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature. 1962 Jul 21;195:281–283. doi: 10.1038/195281a0. [DOI] [PubMed] [Google Scholar]
  17. Setlow P. Purification and properties of a specific proteolytic enzyme present in spores of Bacillus magaterium. J Biol Chem. 1976 Dec 25;251(24):7853–7862. [PubMed] [Google Scholar]
  18. Setlow P., Waites W. M. Identification of several unique, low-molecular-weight basic proteins in dormant spores of clastridium bifermentans and their degradation during spore germination. J Bacteriol. 1976 Aug;127(2):1015–1017. doi: 10.1128/jb.127.2.1015-1017.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Takagi M., Imanaka T., Aiba S. Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus. J Bacteriol. 1985 Sep;163(3):824–831. doi: 10.1128/jb.163.3.824-831.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yuan K., Johnson W. C., Tipper D. J., Setlow P. Comparison of various properties of low-molecular-weight proteins from dormant spores of several Bacillus species. J Bacteriol. 1981 Jun;146(3):965–971. doi: 10.1128/jb.146.3.965-971.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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