Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1986 Jul;167(1):404–406. doi: 10.1128/jb.167.1.404-406.1986

Genetic and molecular analyses of Escherichia coli N-acetylneuraminate lyase gene.

B Kawakami, T Kudo, Y Narahashi, K Horikoshi
PMCID: PMC212894  PMID: 3522555

Abstract

Two plasmids containing the N-acetylneuraminate lyase (NALase) gene (nanA) of Escherichia coli, pNL1 and pNL4, were constructed. Immunoprecipitation analysis indicated that the 35,000-dalton protein encoded in pNL4 was NALase. The synthesis of NALase in E. coli carrying these plasmids was constitutive.

Full text

PDF

Images in this article

405Image
on p.405

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Kato C., Kudo T., Watanabe K., Horikoshi K. Nucleotide sequence of the beta-lactamase gene of alkalophilic Bacillus sp. strain 170. J Gen Microbiol. 1985 Dec;131(12):3317–3324. doi: 10.1099/00221287-131-12-3317. [DOI] [PubMed] [Google Scholar]
  3. Kitada M., Horikoshi K. Sodium ion-stimulated alpha-[1-14C]aminoisobutyric acid uptake in alkalophilic Bacillus species. J Bacteriol. 1977 Sep;131(3):784–788. doi: 10.1128/jb.131.3.784-788.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Mézes P. S., Blacher R. W., Lampen J. O. Processing of Bacillus cereus 569/H beta-lactamase I in Escherichia coli and Bacillus subtilis. J Biol Chem. 1985 Jan 25;260(2):1218–1223. [PubMed] [Google Scholar]
  5. Sancar A., Hack A. M., Rupp W. D. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. doi: 10.1128/jb.137.1.692-693.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Sashihara N., Kudo T., Horikoshi K. Molecular cloning and expression of cellulase genes of alkalophilic Bacillus sp. strain N-4 in Escherichia coli. J Bacteriol. 1984 May;158(2):503–506. doi: 10.1128/jb.158.2.503-506.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Silver R. P., Finn C. W., Vann W. F., Aaronson W., Schneerson R., Kretschmer P. J., Garon C. F. Molecular cloning of the K1 capsular polysaccharide genes of E. coli. Nature. 1981 Feb 19;289(5799):696–698. doi: 10.1038/289696b0. [DOI] [PubMed] [Google Scholar]
  8. Silver R. P., Vann W. F., Aaronson W. Genetic and molecular analyses of Escherichia coli K1 antigen genes. J Bacteriol. 1984 Feb;157(2):568–575. doi: 10.1128/jb.157.2.568-575.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Uchida Y., Tsukada Y., Sugimori T. Purification and properties of N-acetylneuraminate lyase from Escherichia coli. J Biochem. 1984 Aug;96(2):507–522. doi: 10.1093/oxfordjournals.jbchem.a134863. [DOI] [PubMed] [Google Scholar]
  10. Vimr E. R., Troy F. A. Identification of an inducible catabolic system for sialic acids (nan) in Escherichia coli. J Bacteriol. 1985 Nov;164(2):845–853. doi: 10.1128/jb.164.2.845-853.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vimr E. R., Troy F. A. Regulation of sialic acid metabolism in Escherichia coli: role of N-acylneuraminate pyruvate-lyase. J Bacteriol. 1985 Nov;164(2):854–860. doi: 10.1128/jb.164.2.854-860.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES