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. 1986 Jul;167(1):423–426. doi: 10.1128/jb.167.1.423-426.1986

Regulatory mutation that controls nif expression and histidine transport in Azospirillum brasilense.

M Fischer, E Levy, T Geller
PMCID: PMC212899  PMID: 3087965

Abstract

Mutagenesis of Azospirillum brasilense with nitrosoguanidine and selection on ethylenediamine yielded prototrophs which fixed nitrogen in the presence of ammonia. Nitrogenase activity in mutant strains exceeded that of the wild type three- to sixfold. The same mutants were also constitutive for histidine transport. Enzyme activities involved in ammonia assimilation were not affected by the mutation. The data suggest that the mutation occurred at a site which regulates nif and histidine transport functions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames G. F. Two methods for the assay of amino acid transport in bacteria. Methods Enzymol. 1974;32:843–849. doi: 10.1016/0076-6879(74)32085-x. [DOI] [PubMed] [Google Scholar]
  2. Backman K. C., Chen Y. M., Ueno-Nishio S., Magasanik B. The product of glnL is not essential for regulation of bacterial nitrogen assimilation. J Bacteriol. 1983 Apr;154(1):516–519. doi: 10.1128/jb.154.1.516-519.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burris R. H., Okon Y., Albrecht S. L. Physiological studies of Spirillum lipoferum. Basic Life Sci. 1977;9:445–450. [PubMed] [Google Scholar]
  4. Drummond M., Clements J., Merrick M., Dixon R. Positive control and autogenous regulation of the nifLA promoter in Klebsiella pneumoniae. Nature. 1983 Jan 27;301(5898):302–307. doi: 10.1038/301302a0. [DOI] [PubMed] [Google Scholar]
  5. Espin G., Alvarez-Morales A., Cannon F., Dixon R., Merrick M. Cloning of the glnA, ntrB and ntrC genes of Klebsiella pneumoniae and studies of their role in regulation of the nitrogen fixation (nif) gene cluster. Mol Gen Genet. 1982;186(4):518–524. doi: 10.1007/BF00337959. [DOI] [PubMed] [Google Scholar]
  6. Kenealy W. R., Thompson T. E., Schubert K. R., Zeikus J. G. Ammonia assimilation and synthesis of alanine, aspartate, and glutamate in Methanosarcina barkeri and Methanobacterium thermoautotrophicum. J Bacteriol. 1982 Jun;150(3):1357–1365. doi: 10.1128/jb.150.3.1357-1365.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kustu S. G., McFarland N. C., Hui S. P., Esmon B., Ames G. F. Nitrogen control of Salmonella typhimurium: co-regulation of synthesis of glutamine synthetase and amino acid transport systems. J Bacteriol. 1979 Apr;138(1):218–234. doi: 10.1128/jb.138.1.218-234.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kustu S., Burton D., Garcia E., McCarter L., McFarland N. Nitrogen control in Salmonella: regulation by the glnR and glnF gene products. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4576–4580. doi: 10.1073/pnas.76.9.4576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. McFarland N., McCarter L., Artz S., Kustu S. Nitrogen regulatory locus "glnR" of enteric bacteria is composed of cistrons ntrB and ntrC: identification of their protein products. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2135–2139. doi: 10.1073/pnas.78.4.2135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Merrick M. J. Nitrogen control of the nif regulon in Klebsiella pneumoniae: involvement of the ntrA gene and analogies between ntrC and nifA. EMBO J. 1983;2(1):39–44. doi: 10.1002/j.1460-2075.1983.tb01377.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nagatani H., Shimizu M., Valentine R. C. The mechanism of ammonia assimilation in nitrogen fixing Bacteria. Arch Mikrobiol. 1971;79(2):164–175. doi: 10.1007/BF00424923. [DOI] [PubMed] [Google Scholar]
  13. Okon Y., Albrecht S. L., Burris R. H. Factors affecting growth and nitrogen fixation of Spirillum lipoferum. J Bacteriol. 1976 Sep;127(3):1248–1254. doi: 10.1128/jb.127.3.1248-1254.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Okon Y., Houchins J. P., Albrecht S. L., Burris R. H. Growth of Spirillum lipoferum at constant partial pressures of oxygen, and the properties of its nitrogenase in cell-free extracts. J Gen Microbiol. 1977 Jan;98(1):87–93. doi: 10.1099/00221287-98-1-87. [DOI] [PubMed] [Google Scholar]
  15. Pahel G., Tyler B. A new glnA-linked regulatory gene for glutamine synthetase in Escherichia coli. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4544–4548. doi: 10.1073/pnas.76.9.4544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tarrand J. J., Krieg N. R., Döbereiner J. A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can J Microbiol. 1978 Aug;24(8):967–980. doi: 10.1139/m78-160. [DOI] [PubMed] [Google Scholar]

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