Abstract
Two enrichment broths (Preston and Roman & Doyle's) and four solid media (Preston, Skirrow's, Butzler's and Blaser's) were compared to determine their relative efficiencies in recovering campylobacters from 389 freshly voided seagull faeces, 276 of which were found to contain campylobacters by one or more of the procedures used. A combination of enrichment in Preston medium followed by plating on to Preston agar gave the highest number of isolates (263). Enrichment in fluid media was shown to be an important part of the technique, as only 85 (30.8%) of the 276 isolations were made as a result of direct plating. Very little difference was seen between the two forms of enrichment (P greater than 0.5) but of the four selective media, Butzler's was significantly less efficient than any of the other three (P less than 0.01), because it failed to grow more than a few strains of Campylobacter coli and the NARTC group, which together made up nearly two-thirds of the total number od Campylobacter spp. isolated.
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- Blaser M., Cravens J., Powers B. W., Wang W. L. Campylobacter enteritis associated with canine infection. Lancet. 1978 Nov 4;2(8097):979–981. doi: 10.1016/s0140-6736(78)92541-2. [DOI] [PubMed] [Google Scholar]
- Bolton F. J., Coates D., Hinchliffe P. M., Robertson L. Comparison of selective media for isolation of Campylobacter jejuni/coli. J Clin Pathol. 1983 Jan;36(1):78–83. doi: 10.1136/jcp.36.1.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bolton F. J., Hinchliffe P. M., Coates D., Robertson L. A most probable number method for estimating small numbers of campylobacters in water. J Hyg (Lond) 1982 Oct;89(2):185–190. doi: 10.1017/s0022172400070716. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bolton F. J., Robertson L. A selective medium for isolating Campylobacter jejuni/coli. J Clin Pathol. 1982 Apr;35(4):462–467. doi: 10.1136/jcp.35.4.462. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brouwer R., Mertens M. J., Siem T. H., Katchaki J. An explosive outbreak of Campylobacter enteritis in soldiers. Antonie Van Leeuwenhoek. 1979;45(3):517–519. doi: 10.1007/BF00443293. [DOI] [PubMed] [Google Scholar]
- Doyle M. P., Roman D. J. Recovery of Campylobacter jejuni and Campylobacter coli from inoculated foods by selective enrichment. Appl Environ Microbiol. 1982 Jun;43(6):1343–1353. doi: 10.1128/aem.43.6.1343-1353.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fenlon D. R. Seagulls (Larus spp.) as vectors of salmonellae: an investigation into the range of serotypes and numbers of salmonellae in gull faeces. J Hyg (Lond) 1981 Apr;86(2):195–202. doi: 10.1017/s0022172400068911. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoffman P. S., Krieg N. R., Smibert R. M. Studies of the microaerophilic nature of Campylobacter fetus subsp. jejuni. I. Physiological aspects of enhanced aerotolerance. Can J Microbiol. 1979 Jan;25(1):1–7. doi: 10.1139/m79-001. [DOI] [PubMed] [Google Scholar]
- Johnston W. S., MacLachlan G. K., Hopkins G. F. The possible involvement of seagulls (Larus sp) in the transmission of salmonella in dairy cattle. Vet Rec. 1979 Dec 8;105(23):526–527. [PubMed] [Google Scholar]
- Johnston W. S., Munro D., Reilly W. J., Sharp J. C. An unusual sequel to imported Salmonella zanzibar. J Hyg (Lond) 1981 Dec;87(3):525–528. doi: 10.1017/s0022172400069771. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knill M., Suckling W. G., Pearson A. D. Environmental isolation of heat-tolerant Campylobacter in the Southampton area. Lancet. 1978 Nov 4;2(8097):1002–1003. doi: 10.1016/s0140-6736(78)92577-1. [DOI] [PubMed] [Google Scholar]
- Lauwers S., De Boeck M., Butzler J. P. Campylobacter enteritis in Brussels. Lancet. 1978 Mar 18;1(8064):604–605. doi: 10.1016/s0140-6736(78)91045-0. [DOI] [PubMed] [Google Scholar]
- Lucke J. N., Hall G. M., Lister D. Anaesthesia of pigs sensitive to malignant hyperthermia. Vet Rec. 1977 Jan 15;100(3):45–48. doi: 10.1136/vr.100.3.45. [DOI] [PubMed] [Google Scholar]
- Luechtefeld N. A., Blaser M. J., Reller L. B., Wang W. L. Isolation of Campylobacter fetus subsp. jejuni from migratory waterfowl. J Clin Microbiol. 1980 Sep;12(3):406–408. doi: 10.1128/jcm.12.3.406-408.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luechtefeld N. W., Wang W. L. Campylobacter fetus subsp. jejuni in a turkey processing plant. J Clin Microbiol. 1981 Feb;13(2):266–268. doi: 10.1128/jcm.13.2.266-268.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Patton C. M., Mitchell S. W., Potter M. E., Kaufmann A. F. Comparison of selective media for primary isolation of Campylobacter fetus subsp. jejuni. J Clin Microbiol. 1981 Feb;13(2):326–330. doi: 10.1128/jcm.13.2.326-330.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pearson A. D., Suckling W. G., Ricciardi I. D., Knill M., Ware E. Campylobacter-associated diarrhoea in Southampton. Br Med J. 1977 Oct 8;2(6092):955–956. doi: 10.1136/bmj.2.6092.955-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Skirrow M. B., Benjamin J. '1001' Campylobacters: cultural characteristics of intestinal campylobacters from man and animals. J Hyg (Lond) 1980 Dec;85(3):427–442. doi: 10.1017/s0022172400063506. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Skirrow M. B., Fidoe R. G., Jones D. M. An outbreak of presumptive food-borne campylobacter enteritis. J Infect. 1981 Sep;3(3):234–236. doi: 10.1016/s0163-4453(81)90819-7. [DOI] [PubMed] [Google Scholar]
- Svedhem A., Kaijser B., Sjögren E. The occurrence of Campylobacter jejuni in fresh food and survival under different conditions. J Hyg (Lond) 1981 Dec;87(3):421–425. doi: 10.1017/s0022172400069667. [DOI] [PMC free article] [PubMed] [Google Scholar]