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. 1984 Oct;93(2):301–312. doi: 10.1017/s0022172400064834

Interpretation of responses and protective levels of antibody against attenuated influenza A viruses using single radial haemolysis.

R Al-Khayatt, R Jennings, C W Potter
PMCID: PMC2129441  PMID: 6389697

Abstract

Antibody determinations against H3N2 and H1N1 type A influenza viruses were carried out on paired sera obtained from volunteers taking part in influenza virus vaccine studies, using both the haemagglutination-inhibition (HI) and single radial haemolysis (SRH) test. Good correlation between the HI and SRH test was found for both H3N2 and H1N1 antibody and the zone area increases corresponding to significant SRH antibody rises determined for both virus strains. In both H3N2 and H1N1 vaccine studies, intranasal infection of the volunteers with live attenuated viruses was involved and by the measurement of HI and SRH antibodies prior to and following infection, levels of antibody equating with protection against the infecting viruses could be estimated. For the HI test the antibody titres associated with 50% protection were 42 for H1N1, and 44 for H3N2 viruses; for the SRH test, 50% protection was associated with zone areas of 20.0-25.0 mm2 for both H1N1 and H3N2 viruses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chakraverty P. Comparison of haemagglutination-inhibition and single-radial-haemolysis techniques for detection of antibodies to influenza B virus. Arch Virol. 1980;63(3-4):285–289. doi: 10.1007/BF01315034. [DOI] [PubMed] [Google Scholar]
  2. Clark A., Potter C. W., Jennings R., Nicholl J. P., Langrick A. F., Schild G. C., Wood J. M., Tyrrell D. A. A comparison of live and inactivated influenza A (H1N1) virus vaccines. 1. Short-term immunity. J Hyg (Lond) 1983 Jun;90(3):351–359. doi: 10.1017/s0022172400028989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark A., Potter C. W., Jennings R., Nicholl J. P., Langrick A. F., Schild G. C., Wood J. M., Tyrrell D. A. A comparison of live and inactivated influenza A (H1N1) virus vaccines. 2. Long-term immunity. J Hyg (Lond) 1983 Jun;90(3):361–370. doi: 10.1017/s0022172400028990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Creţescu L., Zilisteanu E., Niculescu I. T., Crainic R. Detection of antibody responses to natural influenza infection or vaccination by single radial haemolysis test. Arch Roum Pathol Exp Microbiol. 1979 Jan-Mar;38(1):77–80. [PubMed] [Google Scholar]
  5. Delem A., Jovanovic D. Correlation between rate of infection and preexisting titer of serum antibody as determined by single radial hemolysis during and epidemic of influenza A/Victoria/3/75. J Infect Dis. 1978 Feb;137(2):194–196. doi: 10.1093/infdis/137.2.194. [DOI] [PubMed] [Google Scholar]
  6. Dowdle W. R., Coleman M. T., Mostow S. R., Kaye H. S., Schoenbaum S. C. Inactivated influenza vaccines. 2. Laboratory indices of protection. Postgrad Med J. 1973 Mar;49(569):159–163. doi: 10.1136/pgmj.49.569.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Farník J., Bruj J. An outbreak of influenza A2 in a population with a known antibody profile. J Infect Dis. 1966 Oct;116(4):425–428. doi: 10.1093/infdis/116.4.425. [DOI] [PubMed] [Google Scholar]
  8. Farrohi K., Farrohi F. K., Noble G. R., Kaye H. S., Kendal A. P. Evaluation of the single radial hemolysis test for measuring hemagglutinin- and neuraminidase-specific antibodies to H3N2 influenza strains and antibodies to influenza B. J Clin Microbiol. 1977 Mar;5(3):353–360. doi: 10.1128/jcm.5.3.353-360.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goodeve A. C., Jennings R., Potter C. W. The use of the single radial haemolysis test for assessing antibody response and protective antibody levels in an influenza B vaccine study. J Biol Stand. 1983 Oct;11(4):289–296. doi: 10.1016/s0092-1157(83)80017-1. [DOI] [PubMed] [Google Scholar]
  10. Grilli E. A., Davies J. R. Influenza B at Christ's Hospital: natural antibody to influenza B estimated by radial haemolysis. J Hyg (Lond) 1981 Oct;87(2):211–218. doi: 10.1017/s0022172400069424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hobson D., Curry R. L., Beare A. S., Ward-Gardner A. The role of serum haemagglutination-inhibiting antibody in protection against challenge infection with influenza A2 and B viruses. J Hyg (Lond) 1972 Dec;70(4):767–777. doi: 10.1017/s0022172400022610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jennings R., Denton M. D., Potter C. W. The hamster as an experimental animal for the study of influenza. I. The role of antibody in protection. Med Microbiol Immunol. 1976 Dec 1;162(3-4):217–226. doi: 10.1007/BF02121000. [DOI] [PubMed] [Google Scholar]
  13. Jennings R., Potter C. W., Massey P. M., Duerden B. I., Martin J., Bevan A. M. Responses of volunteers to inactivated influenza virus vaccines. J Hyg (Lond) 1981 Feb;86(1):1–16. doi: 10.1017/s0022172400068698. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jennings R., Smith T. L., Spencer R. C., Mellersh A. M., Edey D., Fenton P., Potter C. W. Inactivated influenza virus vaccines in man: a comparative study of subunit and split vaccines using two methods for assessment of antibody responses. Vaccine. 1984 Mar;2(1):75–80. doi: 10.1016/s0264-410x(98)90036-6. [DOI] [PubMed] [Google Scholar]
  15. Kurtz J. B., Mortimer P. P., Mortimer P. R., Morgan-Capner P., Shafi M. S., White G. B. Rubella antibody measured by radial haemolysis. Characteristics and performance of a simple screening method for use in diagnostic laboratories. J Hyg (Lond) 1980 Apr;84(2):213–222. doi: 10.1017/s0022172400026711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LaMontagne J. R. From the National Institute of Allergy and Infectious Diseases: summary of a workshop on influenza B viruses and Reye's syndrome. J Infect Dis. 1980 Sep;142(3):452–465. doi: 10.1093/infdis/142.3.452. [DOI] [PubMed] [Google Scholar]
  17. MEIKLEJOHN G., KEMPE C. H., THALMAN W. G., LENNETTE E. H. Evaluation of monovalent influenza vaccines. II. Observations during an influenza a-prime epidemic. Am J Hyg. 1952 Jan;55(1):12–21. doi: 10.1093/oxfordjournals.aje.a119500. [DOI] [PubMed] [Google Scholar]
  18. Mancini G., Donatelli I., Arangio-Ruiz G., Rozera C., Macchia T. Comparison of haemagglutination-inhibition and single radial haemolysis techniques for detecting antibodies to influenza A and B viruses. J Hyg (Lond) 1983 Aug;91(1):157–162. doi: 10.1017/s0022172400060137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Oxford J. S., Yetts R., Schild G. C. Quantitation and analysis of the specificity of post-immunization antibodies to influenza B viruses using single radial haemolysis. J Hyg (Lond) 1982 Apr;88(2):325–333. doi: 10.1017/s0022172400070170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Potter C. W., Jennings R., Nicholson K., Tyrrell D. A., Dickinson K. G. Immunity to attenuated influenza virus WRL 105 infection induced by heterologous, inactivated influenza A virus vaccines. J Hyg (Lond) 1977 Dec;79(3):321–332. doi: 10.1017/s0022172400053158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Potter C. W., Oxford J. S. Determinants of immunity to influenza infection in man. Br Med Bull. 1979 Jan;35(1):69–75. doi: 10.1093/oxfordjournals.bmb.a071545. [DOI] [PubMed] [Google Scholar]
  22. Pyhälä R., Aho K. Serum HI antibody and protection against influenza: a follow-up survey at community level of three epidemics caused by different H3N2-variants. Int J Epidemiol. 1975 Jun;4(2):127–129. doi: 10.1093/ije/4.2.127. [DOI] [PubMed] [Google Scholar]
  23. Russell S. M., McCahon D., Beare A. S. A single radial haemolysis technique for the measurement of influenza antibody. J Gen Virol. 1975 Apr;27(1):1–10. doi: 10.1099/0022-1317-27-1-1. [DOI] [PubMed] [Google Scholar]
  24. Schild G. C., Pereira M. S., Chakraverty P. Single-radial-hemolysis: a new method for the assay of antibody to influenza haemagglutinin. Applications for diagnosis and seroepidemiologic surveillance of influenza. Bull World Health Organ. 1975;52(1):43–50. [PMC free article] [PubMed] [Google Scholar]
  25. Stuart-Harris C. The epidemiology and prevention of influenza. Am Sci. 1981 Mar-Apr;69(2):166–172. [PubMed] [Google Scholar]
  26. Vänänen P., Hovi T., Helle E. P., Penttinen K. Determination of mumps and influenza antibodies by haemolysis-in-gel. Arch Virol. 1976;52(1-2):91–99. doi: 10.1007/BF01317868. [DOI] [PubMed] [Google Scholar]
  27. Wright P. F., Bryant J. D., Karzon D. T. Comparison of influenza B/Hong Kong virus infections among infants, children, and young adults. J Infect Dis. 1980 Apr;141(4):430–435. doi: 10.1093/infdis/141.4.430. [DOI] [PubMed] [Google Scholar]
  28. Yamagishi H., Nagamine T., Shimoda K., Ide S., Igarashi Y., Yoshioka I., Matumoto M. Comparative measurement of equine influenza virus antibodies in horse sera by single radial hemolysis, neutralization, and hemagglutination inhibition tests. J Clin Microbiol. 1982 Apr;15(4):660–662. doi: 10.1128/jcm.15.4.660-662.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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