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. 1976 Jun;76(3):429–439. doi: 10.1017/s0022172400055364

A new Pseudomonas vaccine: preliminary trial on human volunteers.

R J Jones, E A Roe, E J Lowbury, J J Miller, J F Spilsbury
PMCID: PMC2129666  PMID: 819576

Abstract

Fifteen healthy volunteers were given three weekly subcutaneous injections of a new polyvalent Pseudomonas aeruginosa vaccine (PEV-01). Four doses - 1-0 RHD (manufacturer's recommended human dose), 0-75 RHD, 0-5 RHD and 0-1 RHD - were used in separate groups of volunteers. Blood samples taken before each of the injections and one taken 7 days after the last injection were examined for immune response to the vaccine and for possible adverse clinical, biochemical and haematological effects. Raised titres of antibody in serum of volunteers given 0-5-1-0 RHD vaccine were shown, often by the seventh day, in passive haemagglutination tests against all of the 16 serotypes of Ps. aeruginosa represented in the vaccine; serum from volunteers who received 0-1 RHD usually showed a reduced antibody titre. Tests of mouse protection by serum against intraperitoneal challenge with Ps. aeruginosa P14 showed increased titres of mouse protective antibody in the blood of volunteers given 1-0, 0-75 or 0-5 RHD of vaccine but a reduced mouse-protective titre in two out of three sera from volunteers given 0-1 RHD vaccine. There was a suggestion of enhanced phagocytic ingestion and intracellular killing of two strains of Ps. aeruginosa by the blood of vaccinated volunteers, and more definite enhancement of ingestion of inert latex particles, which were less well ingested than were the bacterial cells by phagocytes from unvaccinated volunteers. Apart from slight or moderate local reactions and a transient rise of temperature in some volunteers, there were no clinical, biochemical or haematological abnormalities in the vaccinated volunteers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FOX J. E., LOWBURY E. J. Immunity and antibody to Pseudomonas pyocyanea in rabbits. J Pathol Bacteriol. 1953 Apr;65(2):533–542. doi: 10.1002/path.1700650225. [DOI] [PubMed] [Google Scholar]
  2. Gordon P. A., Stuart J., Lee T. R., Breeze G. R., Pugh R. N. The cytocentrifuge NBT test. J Clin Pathol. 1975 Aug;28(8):674–679. doi: 10.1136/jcp.28.8.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Jones R. J., Dyster R. E. The role of polymorphonuclear leucocytes in protecting mice vaccinated against Pseudomon as aeruginosa infections. Br J Exp Pathol. 1973 Aug;54(4):416–421. [PMC free article] [PubMed] [Google Scholar]
  4. Jones R. J. Early protection by vaccines in burns. Br J Exp Pathol. 1971 Feb;52(1):100–109. [PMC free article] [PubMed] [Google Scholar]
  5. Jones R. J., Hall M., Ricketts C. R. Passive protective properties of serum fractions from mice inoculated with an anti-pseudomonas vaccine. Immunology. 1972 Dec;23(6):889–895. [PMC free article] [PubMed] [Google Scholar]
  6. Jones R. J., Jackson D. M., Lowbury E. J. Antiserum and antibiotic in the prophylaxis of burns against Pseudomonas aeruginosa. Br J Plast Surg. 1966 Jan;19(1):43–57. doi: 10.1016/s0007-1226(66)80007-3. [DOI] [PubMed] [Google Scholar]
  7. Jones R. J., Lilly H. A., Lowbury E. J. Passive protection of mice against Pseudomonas aeruginosa by serum from recently vaccinated mice. Br J Exp Pathol. 1971 Jun;52(3):264–270. [PMC free article] [PubMed] [Google Scholar]
  8. Jones R. J., Lowbury E. J. Early protection by vaccines against Pseudomonas aeruginosa colonizing burns. Br J Exp Pathol. 1972 Dec;53(6):659–664. [PMC free article] [PubMed] [Google Scholar]
  9. Jones R. J. Protection against Pseudomonas aeruginosa infection by immunisation with fractions of culture filtrates of Ps. aeruginosa. Br J Exp Pathol. 1968 Oct;49(5):411–420. [PMC free article] [PubMed] [Google Scholar]
  10. Jones R. J., Roe E. A., Dyster R. E. Detection of endotoxins with the Limulus test in burned and unburned mice infected with different species of gram-negative bacteria. J Hyg (Lond) 1975 Aug;75(1):99–112. doi: 10.1017/s0022172400047112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jones R. J., Roe E. A. Protective properties and haemagglutinins in serum from humans and in serum from mice injected with a new polyvalent Pseudomonas vaccine. Br J Exp Pathol. 1975 Feb;56(1):34–43. [PMC free article] [PubMed] [Google Scholar]
  12. Levin J., Bang F. B. Clottable protein in Limulus; its localization and kinetics of its coagulation by endotoxin. Thromb Diath Haemorrh. 1968 Mar 31;19(1):186–197. [PubMed] [Google Scholar]
  13. Young L. S., Armstrong D. Human immunity to Pseudomonas aeruginosa. I. In-vitro interaction of bacteria, polymorphonuclear leukocytes, and serum factors. J Infect Dis. 1972 Sep;126(3):257–276. doi: 10.1093/infdis/126.3.257. [DOI] [PubMed] [Google Scholar]

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