Skip to main content
PMC home page
The Journal of Hygiene logoLink to The Journal of Hygiene
. 1978 Aug;81(1):107–123. doi: 10.1017/s002217240005381x

Microneutralization tests for serological typing and subtyping of foot-and-mouth disease virus strains.

M M Rweyemamu, J C Booth, M Head, T W Pay
PMCID: PMC2129748  PMID: 211160

Abstract

A microneutralization test for serotyping of FMD viruses is described. It is based on earlier observations by Booth, Rweyemamu & Pay (1978) that dose-response relationships in quantal microneutralizations often deviated from linearity. The typing test described therefore utilizes undiluted virus preparations. In about 90% of samples a positive typing was obtained in contrast with about 50% for the complement fixation test. The test was also found to be susceptible to minimal quantities of heterotypic viral contamination. For strain differentiation the microneutralization test was carried out as a checkerboard test. When compared with the complement fixation test it was found to be more specific. The necessity to utilize virus-neutralization test systems for comparing FMD virus strains particularly for the purpose of vaccine selection is emphasized. The two dimensional microneutralization test has been applied to a study of comparing FMDV vaccine strains for Europe, South America, the Middle East and East Africa.

Full text

PDF
123

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BROWN F., CRICK J. Application of agar gel precipitin tests to the study of the virus of foot-and-mouth disease. Virology. 1958 Feb;5(1):133–144. doi: 10.1016/0042-6822(58)90010-2. [DOI] [PubMed] [Google Scholar]
  2. Booth J. C., Rweyemamu M. M., Pay T. W. Dose-response relationships in a microneutralization test for foot-and-mouth disease viruses. J Hyg (Lond) 1978 Feb;80(1):31–42. doi: 10.1017/s0022172400053377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown F., Smale C. J. Demonstration of three specific sites on the surface of foot-and-mouth disease virus by antibody complexing. J Gen Virol. 1970;7(2):115–127. doi: 10.1099/0022-1317-7-2-115. [DOI] [PubMed] [Google Scholar]
  4. CAPSTICK P. B., SELLERS R. F., STEWART C. L. The neutralisation of the virus of the foot-and-mouth disease by immune serum. Arch Gesamte Virusforsch. 1960;9:606–620. doi: 10.1007/BF01242147. [DOI] [PubMed] [Google Scholar]
  5. Cowan K. M. Antibody response to viral antigens. Adv Immunol. 1973;17:195–253. doi: 10.1016/s0065-2776(08)60733-6. [DOI] [PubMed] [Google Scholar]
  6. Cowan K. M., Graves J. H. A third antigenic component associated with foot-and-mouth disease infection. Virology. 1966 Nov;30(3):528–540. doi: 10.1016/0042-6822(66)90128-0. [DOI] [PubMed] [Google Scholar]
  7. Cowan K. M., Wagner G. G. Immunochemical studies of foot-and-mouth disease. 8. Detection and quantitation of antibodies by radial immunodiffusion. J Immunol. 1970 Sep;105(3):557–566. [PubMed] [Google Scholar]
  8. DAVIE J. A COMPLEMENT FIXATION TECHNIQUE FOR THE QUANTITATIVE MEASUREMENT OF ANTIGENIC DIFFERENCES BETWEEN STRAINS OF THE VIRUS OF FOOT-AND-MOUTH DISEASE. J Hyg (Lond) 1964 Dec;62:401–411. doi: 10.1017/s0022172400040146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Forman A. J. A comparison of some immunological methods for the differentiation of strains of foot-and-mouth disease virus. J Hyg (Lond) 1975 Apr;74(2):215–225. doi: 10.1017/s002217240002427x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Forman A. J. A study of foot-and-mouth disease virus strains by complement fixation. I. A model for the fixation of complement by antigen-antibody mixtures. J Hyg (Lond) 1974 Jun;72(3):397–405. doi: 10.1017/s0022172400023639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GRAVES J. H. The differentiation of subtypes (variants) of foot-and-mouth disease virus by serologic methods. I. Complement-fixation test. Am J Vet Res. 1960 Jul;21:687–690. [PubMed] [Google Scholar]
  12. GRAVES J. H. The differentiation of subtypes (variants) of foot-and-mouth disease virus by serologic methods. II. Precipitin test in agar gel. Am J Vet Res. 1960 Jul;21:691–693. [PubMed] [Google Scholar]
  13. Lobo C. A., Cowan K. M., Trautman R., Hanson R. P. Differentiation of type A foot-and-mouth disease virus subtypes by double- and radial-immunodiffusion analysis. Am J Vet Res. 1974 Aug;35(8):1121–1126. [PubMed] [Google Scholar]
  14. Martinsen J. S. Two-plaque-size variants of foot-and-mouth disease virus differing in neutralization by guinea-pig antisera. Res Vet Sci. 1971 Jul;12(4):399–400. [PubMed] [Google Scholar]
  15. McVicar J. W., Sutmoller P., Andersen A. A. Foot-and-mouth disease virus: plaque reduction neutralization tests. Arch Gesamte Virusforsch. 1974;44(2):168–172. doi: 10.1007/BF01250230. [DOI] [PubMed] [Google Scholar]
  16. Pay T. W., Parker M. J. Some statistical and experimental design problems in the assessment of FMD vaccine potency. Dev Biol Stand. 1976;35:369–383. [PubMed] [Google Scholar]
  17. Pereira H. G. Subtyping of foot-and-mouth disease virus. Dev Biol Stand. 1976;35:167–174. [PubMed] [Google Scholar]
  18. Rowlands D. J., Cartwright B., Brown F. Evidence for an internal antigen in foot-and-mouth disease virus. J Gen Virol. 1969 Jun;4(4):479–487. doi: 10.1099/0022-1317-4-4-479. [DOI] [PubMed] [Google Scholar]
  19. Rweyemamu M. M., Booth J. C., Parry N., Pay T. W. Neutralization kinetics studies with type SAT 2 foot-and-mouth disease virus strains. J Hyg (Lond) 1977 Jun;78(3):429–438. doi: 10.1017/s0022172400056321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rweyemamu M. M., Pay T. W., Parker M. J. Serological differentiation of foot-and-mouth disease virus strains in relation to selection of suitable vaccine viruses. Dev Biol Stand. 1976;35:205–204. [PubMed] [Google Scholar]
  21. Sugimura T., Eissner G. Typing foot-and-mouth disease virus by fluorescent antibody technique. Natl Inst Anim Health Q (Tokyo) 1976 Winter;16(4):152–159. [PubMed] [Google Scholar]
  22. Wild T. F., Burroughs J. N., Brown F. Surface structure of foot-and-mouth disease virus. J Gen Virol. 1969 Apr;4(3):313–320. doi: 10.1099/0022-1317-4-3-313. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Hygiene are provided here courtesy of Cambridge University Press

RESOURCES